LIU Li xin,TIAN Yi dan,WU Yuan yuan,et al.Effect of Simulated Acid Rain on Antioxidative Characteristics in Different Genotypes Soybean[J].Soybean Science,2014,33(01):41-45.[doi:10.11861/j.issn.1000-9841.2014.01.0041]
模拟酸雨对不同基因型大豆抗氧化系统的影响
- Title:
- Effect of Simulated Acid Rain on Antioxidative Characteristics in Different Genotypes Soybean
- 文章编号:
- 1000-9841（2014）01-0041-05
- Keywords:
- Simulated acid rain; Soybean; Reactive oxygen species level; Antioxidant enzymes; Antioxidants
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以南农1138-2和科丰1号为试验材料，通过盆栽试验研究了4种pH酸雨处理对大豆叶片抗氧化系统的影响。结果表明：中度酸雨（pH≥3.5）处理，除对南农1138-2的POD活性影响显著外，对2个品种的活性氧水平，抗氧化酶、非酶系统和MDA含量影响均不显著，且在苗期和开花期均保持比较稳定的状态，南农1138-2略有减产，但科丰1号增产显著；强酸雨(pH2.5)胁迫后，活性氧水平升高，活性氧产生与清除的代谢系统平衡被破坏，丙二醛含量增加，膜脂过氧化加剧，且随处理次数增加伤害程度加重，产量降低显著，其中科丰1号产量降幅较大，受害程度大于南农1138-2。综合分析表明，中度酸雨处理，科丰1号较南农1138-2有更好的耐酸雨性，而强酸雨胁迫下，科丰1号的受伤害程度较南农1138-2严重。
- Abstract:
- Nannong1138-2 and Kefeng 1 were used to study the effects of simulated acid rain(SAR)with different pH on the physiological characteristics in leaves of soybean.The results showed that the moderate acidity of SAR(pH≥3.5)had no significant effects on the level of reactive oxygen species,antioxidant enzymes,non-enzymatic systems and MDA content except the POD activity of Nannong1138-2,and remained relatively stable during growing period.The yield of Nannong1138-2 decreased,while that of Kefeng 1 increased significantly at pH3.5 and pH 4.5 treatments.High acidity of SAR(pH2.5)increased reactive oxygen species level,antioxidant enzyme activities and MDA content.The damage of pH2.5 SAR increased with the treatment times and finally caused yield reduction.Kefeng 1 was hurt more than Nannong 1138-2 at high acidity of SAR.In conclusion,Kefeng 1 had better acid rain resistance under the moderate acid rain stress,while worse resistance under high acidity of SAR stress.
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备注/Memo
基金项目：国家“十一五”科技支撑计划 (2009BADA8B02)；江苏省重点科技支撑与自主创新示范工程(BE2008618)。
第一作者简介：刘丽欣（1987-），女，硕士，主要从事作物逆境生理研究。E-mail:2010101041@njau.edu.cn。
通讯作者：江海东（1968），男，博士，副教授，主要从事作物生理生态研究。E-mail:hdjiang@njau.edu.cn。