[1]苗兴芬,李灿东,郑殿峰,等.大豆油酸含量相关QTL间的上位效应和QE互作效应[J].大豆科学,2014,33(01):23-26.[doi:10.11861/j.issn.1000-9841.2014.01.0023]
　MIAO Xingfen,LI Candong,ZHENG Dianfeng,et al.Epistatic Effects of QTLs and QE Interaction Effects on Oleic Acid Content in Soybean[J].Soybean Science,2014,33(01):23-26.[doi:10.11861/j.issn.1000-9841.2014.01.0023]
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大豆油酸含量相关QTL间的上位效应和QE互作效应

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年01期 页码: 23-26 栏目: 出版日期: 2014-02-25
Title:
Epistatic Effects of QTLs and QE Interaction Effects on Oleic Acid Content in Soybean
文章编号:
10009841（2014）01002304
作者:
苗兴芬1; 2; 3 ; 李灿东4 ; 郑殿峰1 ; 蒋洪蔚3 ; 刘春燕2; 3 ; 辛大伟2 ; 陈庆山2; 3 ; 胡国华2; 3
1.黑龙江八一农垦大学 农学院,黑龙江 大庆 163319；
?2.东北农业大学 农学院,黑龙江 哈尔滨 150030；
3.黑龙江省农垦科研育种中心,黑龙江 哈尔滨 150090；
4.黑龙江省农业科学院 佳木斯分院,黑龙江 佳木斯 154007
Author(s):
MIAO Xingfen 1; 2; 3 ; LI Candong 4 ; ZHENG Dianfeng 1 ; JIANG Hongwei 3 ; LIU Chunyan 2; 3 ; XIN Dawei 2 ;  CHEN Qingshan 2; 3 ; HU Guohua 2; 3
1.College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319,China;2.Land Reclamation Research & Breeding Center of Heilongjiang,Harbin 150090,China;3.College of Agriculture,Northeast Agricultural University,Harbin 150030,China;4.Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences,Jiamusi 154007,China
关键词:
大豆; 油酸含量; 混合线性模型; QTL与环境互作效应; 上位互作效应
Keywords:
Soybean; Oleic acid content; Mixed linear model; QTL×environment interaction; Epistatic effects
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.01.0023
文献标志码:
A
摘要:
利用Charleston×东农594重组自交系构建的SSR遗传图谱,并运用混合线性模型方法对20072009年的大豆油分含量进行QTL定位,并作加性效应,加性×加性上位互作效应及环境互作效应分析。共检测到7个QTL,有6个与环境互作,位于A1、D1a和D1b连锁群上,解释该性状总变异的50.18%,与环境互作贡献率为11.78% 。另检测到12对影响油酸含量的加性×加性上位互作效应的QTL,分布于14个连锁群之间。
Abstract:
In this study,QTL of soybean oleic acid content in three years was analyzed with a recombination inbred lines(RIL)population derived from Charleston×Dongnong 594 by mixed linear model approach.Seven QTL with additive effects,including 6 QTLs interacting with environment for oleic acid content were mapped in the linkage groups A1,D1a and D1b,the general phenotypic variation was 50.18%,and the general contribution was 11.78% from QTL×environment interaction.Twelve QTL pairs with epistatic effects for oleic acid content in the RIL were detected among 14 linkage groups.

参考文献/References:

[1]Song Q J,Marek L F,Shoemaker R C,et al.A new integrated genetic linkage map of the soybean[J].Theoretical and Applied Genetics,2004,109:122128.
[2] 吴晓雷,王永军,贺超英,等.大豆重要农艺性状的QTL分析[J].遗传学报，2001，28(10):947955.（Wu X L,Wang Y J,He C Y,et al.QTLs mapping of some agronomic traits of soybean[J].Acta Genetica Sinica,2001,28(10):947955.）
[3] Brummer E C,Graef G L,Orf J,et al.Mapping QTL for seed protein and oil content in eight soybean populations[J].Crop Science,1997,37:370378.
[4] Chapman A,Pantalone V R,Ustun A,et al.Quantitative trait loci for agronomic and seed quality traits in an F2 and F∶6 soybean population[J].Euphytica,2003,129:387393.
[5] Hyten D L,Pantalone V R,Sams C E,et al.Seed quality QTL in a prominent soybean population[J].Theoretical and Applied Genetics,2004,109:552561.
[6] Panthee D R,Pantalone V R,West D R,et al.Quantitative trait loci for protein and oil concentration,and seed size in soybean[J].Crop Science,2005,45:20152022.?
[7] 朱军.广义遗传模型与数量遗传分析新方法[J].浙江农业大学学报,1994,20(6):551559.(Zhu J.General genetic models and new analysis methods for quantitative traits[J].Journal of Zhejiang Agricultural University,1994,20(6):551559.)
[8] Wang D L,Zhu J,Li Z K,et al.Mapping QTLs with epistatic effects and QTLenvironment interactions by mixed linear model approaches[J] .Theoretical and Applied Genetics,1999,99:12551264.?
[9] 袁爱平,曹立勇,庄杰云,等.水稻株高、抽穗期和有效穗数的QTL与环境的互作分析[J].遗传学报,2003,30(10):899906.(Yuan A P,Cao L Y,Zhuang J Y,et al.Analysis of additive and AE interaction effects of QTLs controlling plant height,heading date and panicle number in rice[J].Acta Genetica Sinica,2003,30(10):899906.)
[10]李泽福,周彤,郑天清,等.水稻抽穗期QTL与环境互作分析[J].作物学报,2002,28(6):771-776.(Li Z F,Zhou T,Zheng T Q,et al.Analysis of QTL×environment interactions for heading date of rice[J].Acta Agronomica Sinica,2002,28(6):771776.)
[11] 高用明,朱军,宋佑胜,等.水稻永久F2 群体抽穗期QTL的上位性及其与环境互作效应的分析[J].作物学报,2004,30(9):849854.(Gao Y M,Zhu J,Song Y S,et al.Use of permanent F2 population to analyze epistasis and their interaction effects with environments for QTLs controlling heading date in rice[J].Acta Agronomica Sinica,2004,30(9):849854.)
[12] 张焦平,江良荣,黄建勋,等.水稻抽穗期上位效应和QE互作效应的分析[J].分子植物育种,2006,4(3):351357.(Zhang J P,Jiang L R,Huang J X,et al.Analysis of epistatis and QE interaction effects of QTL controlling heading date in rice[J].Molecular Plant Breeding,2006,4(3):351357.)
[13] 单大鹏,齐照明,陈庆山,等.大豆油分含量相关的QTL间的上位效应和QE互作效应[J].作物学报,2008,34(6):952957.（Shan D P,Qi Z M,Chen Q S,et al.Epistatic effects of QTLs and QE interaction effects on oil content in soybean[J].Acta Agronomica Sinica,2008,34(6):952957.)
[14] 单大鹏,朱荣胜,陈庆山,等.大豆蛋白质含量相关的QTL间的上位效应和QE互作效应[J].作物学报,2009,35(1):4147.(Shan D P,Zhu R S,Chen Q S,et al.Epistatic effects and QE interaction effects of QTLs for protein content in soybean[J].Acta Agronomica Sinica,2009,35(1):4147.)
[15] 苗兴芬,朱命喜,徐文平,等.大豆脂肪酸含量的 QTL 分析[J].作物学报,2010,36(9):14981505.(Miao X F,Zhu M X,Xu W P,et al.QTL analysis of fatty acids contents in soybean[J].Acta Agronomica Sinica,2010,36(9):14981505.)
[16] 王道龙,朱军,黎志康.QTLMaper1.6.http://ibi.zju.edu.cn/software/qtlmapper/index.htm.(Wang D L,Zhu J,Li Z K.QTLMaper1.6.http://ibi.zju.edu.cn/software/qtlmapper/index.htm.)
[17] 苗兴芬,徐文平,陈庆山.大豆脂肪酸组分的快速气相色谱分析[J].大豆科学,2010,29(2):358360.(Miao X F,Xu W P,Chen Q S.Rapid determination on fatty acids content by gas chromatography in soybean[J].Soybean Science,2010,29(2):358-360.)
[18] 陈庆山,张忠臣,刘春燕,等.应用Charleston×东农594重组自交系群体构建SSR大豆遗传图谱[J].中国农业科学,2005,38(7):1312-1316.(Chen Q S,Zhang Z C,Liu C Y,et al.Construction and analysis of soybean genetic map using recombinant inbred line of Charleston×Dongnong 594[J].Scientia Agricultura Sinica,2005,38(7):1312-1316.)
[19] Cregan P B,Jarvik T,Bush A L,et al.An integrated genetic linkage map of the soybean genome[J].Crop Science,1999,39:14641490.
[20] Fan C C,Yu X Q,Xing X Y,et al.The main effects,epistatic effects and environmental interactions of QTLs on the cooking and eating quality of rice in a doublehaploid line population[J].Theoretical and Applied Genetics,2005,110:14451452.

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备注/Memo

基金项目： 黑龙江八一农垦大学作物重点学科项目（ZWXQDJ3）；黑龙江八一农垦大学博士启动金项目；黑龙江省教育厅新世纪优秀人才项目（1252NCET004）。
第一作者简介： 苗兴芬（1975），女，博士，副教授，主要从事大豆遗传育种研究。Email:byndmxf@126.com。
通讯作者：陈庆山（1973），男，教授，主要从事大豆分子生物技术遗传育种研究。Email:qshchen@sohu.com。
胡国华（1969），男，研究员，主要从事大豆遗传育种研究。Email:hugh757@vip.163.com。

更新日期/Last Update: 2014-08-02