CHEN Yun,ZHENG Huan-ming,ZHANG Jing,et al.Hemin-based Combinations of Exogenous Substances Alleviate the Inhibition of Soybean Seed Germination under Salt Stress[J].Soybean Science,2013,32(05):640-646.[doi:10.11861/j.issn.1000-9841.2013.05.0640]
以hemin为基础的物质联用缓解盐胁迫对大豆种子萌发的抑制
- Title:
- Hemin-based Combinations of Exogenous Substances Alleviate the Inhibition of Soybean Seed Germination under Salt Stress
- Keywords:
- Soybean; Salt stress; Seed germination; Germination rate; Hemin; Amylase; Peroxidase
- 文献标志码:
- A
- 摘要:
- ?为有效缓解盐胁迫对大豆种子萌发的抑制，采用hemin与CaCl2、水杨酸（SA）、脯氨酸（Pro）和KNO3等物质的组合，对苏豆5号大豆种子的发芽率、发芽指数以及根与下胚轴的长度进行测定分析。结果表明：在盐胁迫（150 mmol·L-1NaCl）条件下，大豆种子的发芽率、发芽指数等指标均有明显下降，而一定浓度的CaCl2、SA、Pro、KNO3和hemin均能缓解萌发抑制；CaCl2、Pro与hemin联用处理对缓解大豆种子盐胁迫下萌发抑制比其单独处理具有更好的效果，可有效提高大豆种子在盐胁迫条件下的平均根长、发芽率和发芽指数。进一步研究发现，联用处理还有助于提高大豆种子萌发过程中的淀粉酶和过氧化物酶活性，其中120 μmol·L-1 hemin+1.5 mmol·L-1 CaCl2与80 μmol·L-1 hemin+10 mmol·L-1Pro为最佳联用浓度。
- Abstract:
- ?The aim of this study was to investigate a heminbased combination with other substances like CaCl2,salicylic acid(SA),proline(Pro)or KNO3 that could alleviate salt damage during soybean seed germination.In the experiment,soybean seeds of Sudou 5 from Jiangsu Academy of Agricultural Science,were used upon 150?mmol·L-1 NaCl.Subsequently,seed germination rate and index,the growth of radicle and hypocotyl of soybean,were measured and analyzed.The results showed that seed germination,index and the growth of radicle and hypocotyl of soybean were reduced under salt stress,while individually applied CaCl2,salicylic acid(SA),proline,KNO3 and hemin could all alleviate above salt damage.Furthermore,the mixture of hemin plus?CaCl2 or proline was more effective in alleviating salt toxicity.Meanwhile,both of them could enhance amylase and peroxidase(POD)activities.Together,these results clearly indicated that combinations of hemin and CaCl2 or proline exerted an advantageous effect on enhancing salt tolerance during soybean seed germination,for which 120 μmol·L-1 hemin together with 1.5 mmol·L-1mol CaCl2 and 80 μmol·L-1 hemin plus 10 mmol·L-1 proline were the best combinations.
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备注/Memo
?国家自然科学基金（J1210056，J1310015）；国家大学生创新实践计划（1110307xl01）；江苏省科技支撑计划（BE2010382）。