[1]于志晶,蔡勤安,刘艳芝,等.拟南芥抗逆基因DREB2A转化大豆的研究[J].大豆科学,2013,32(05):606-608.[doi:10.11861/j.issn.1000-9841.2013.05.0606]
　YU Zhi-jing,CAI Qin-an,LIU Yan-zhi,et al.Transformation of Soybean with Stress Related Gene DREB2A from Arabidopsis[J].Soybean Science,2013,32(05):606-608.[doi:10.11861/j.issn.1000-9841.2013.05.0606]
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拟南芥抗逆基因DREB2A转化大豆的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年05期 页码: 606-608 栏目: 出版日期: 2013-10-25
Title:
Transformation of Soybean with Stress Related Gene DREB2A from Arabidopsis
作者:
于志晶; 蔡勤安; 刘艳芝; 齐广勋; 马 瑞
吉林省农业科学院 农业生物技术研究所
Author(s):
?YU Zhi-jing; CAI Qin-an; LIU Yan-zhi; QI Guang-xun; MA Rui
关键词:
大豆; DREB2A; 耐盐耐旱; 农杆菌介导遗传转化
Keywords:
Soybean;  DREB2A;  Salt and drought tolerance;  Agrobacterium-mediated transformation
DOI:
10.11861/j.issn.1000-9841.2013.05.0606
文献标志码:
A
摘要:
为提高大豆的耐旱耐盐碱性，将拟南芥转录因子DREB2A序列优化后，构建了植物表达载体pCAMBIA3300-DREB2A，利用农杆菌介导法将DREB2A基因导入大豆品种Willimas 82和Bert中，转化率分别为0.8%和3.2%。对抗性转基因植株进行PCR检测，2个品种分别获得阳性植株28和63株，表明DREB2A已整合到大豆基因组中。

Abstract:
?To improve the salt and drought tolerance of soybean,a vector of pCAMBIA3300-DREB2A with stress related gene DREB2A from Arabidopsis thaliana was constructed. DREB2A gene was introduced into soybean varieties Williams 82 and Bert by Agrobacterium-mediated transformation.The average transformation frequencies were 0.8% and 3.2%，respectively.Twentyeight and 63 positive plants respectively were obtained by PCR identification.The results indicated that the gene DREB2A had been integrated into the genome of soybean.

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备注/Memo

?转基因生物新品种培育重大专项（2011ZX08004-002）。

更新日期/Last Update: 2013-11-01