[1]王 东,宋 君,叶先林,等.转基因大豆外源基因NOS终止子定量测定的不确定度分析[J].大豆科学,2013,32(05):601-605.[doi:10.11861/j.issn.1000-9841.2013.05.0601]
　WANG Dong,SONG Jun,YE Xian-lin,et al.[J].Soybean Science,2013,32(05):601-605.[doi:10.11861/j.issn.1000-9841.2013.05.0601]
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转基因大豆外源基因NOS终止子定量测定的不确定度分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年05期 页码: 601-605 栏目: 出版日期: 2013-10-25
Title:
作者:
王 东; 宋 君; 叶先林; 雷绍荣; 刘文娟; 常丽娟; 尹 全; 张富丽
?四川省农业科学院 分析测试中心
Author(s):
?WANG Dong; SONG Jun; YE Xian-lin; LEI Shao-rong; LIU Wen-juan; CHANG Li-juan; YIN Quan; ZHANG Fu-li
关键词:
转基因大豆; NOS终止子含量; 不确定度; 评估
Keywords:
Genetically modified soybean(GTS40-3-2); Content of NOS terminator; Measurement uncertainty; Evaluation
DOI:
10.11861/j.issn.1000-9841.2013.05.0601
文献标志码:
A
摘要:
?为了解转基因成分定量分析结果不确定度的主要贡献因素，提高转基因成分定量检测质量，用含转基因大豆GTS40-3-2样品进行了NOS终止子含量的测定，并对其测量不确定度进行了初步估算。NOS终止子测量不确定度估算结果：A类不确定度（uA）为0.002，B类不确定度（uB）为0.002，合成不确定度（uC）为0.003；在置信水准（P）为95%的条件下，包含因子（k）取1.96，测量结果的扩展不确定度（U）为0.016；测量结果（C）为0.032±0.016，接近约定真值（0.03），测量不确定度相对较小，检测质量较高。不确定度评估结果表明试验中的微量液体转移的偏差是测量不确定度的主要贡献者，在以后的检测中应重点注意降低微量液体转移的偏差。
〖HT5”H〗关键词：〖HT5”SS〗

Abstract:
?Uncertainty of Testing Content of NOS Terminator from Genetically Modified Soybean Samples by Realtime Quantitative PCR
A preliminary evaluation of measurement uncertainty of NOS terminator from transgenic soybean samples was made in this paper to understand the main contributors among sources of uncertainty and to further increase the quality of testing genetically modified organism(GMO)in future experiments.The measurement uncertainty estimates showed uncertainty of Type A(uA),Type B(uB)and combined uncertainty(uC)was 0.002,0.002 and 0.003,respectively.When confidence level(P)was 95% and the coverage factor(k)was 1.96,the expanded uncertainty(U)was 0.016.Consequently,content of NOS terminator in this measurement was 0.032 ± 0.016,close to the conventional true value(0.03).The measurement uncertainty was relatively small,reflecting a high quality of detection.Measurement uncertainty of NOS terminator indicated the trace amount of liquid transfer was the major source of measurement uncertainty in this experiment.Thus,the trace amount of liquid transfer should be noted in future detection.

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备注/Memo

?四川省质量技术监督局标准化技术体系（ZYBZ2013-39）。

更新日期/Last Update: 2013-11-01