LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46-55.[doi:10.3969/j.issn.1000-9841.2013.01.011]
耐酸铝大豆品种资源的筛选与鉴定
- Title:
- Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum
- 文章编号:
- 1000-9841(2013)01-0046-04
- Keywords:
- Soybean; Aluminum toxicity; Hydroponics method; Potted culture method
- 分类号:
- S565.1
- 摘要:
- 对引进的139份主要来自湖南省的大豆种质资源,苗期采用简单钙溶液法和全营养液法,以主根相对伸长率为评价指标,分析大豆对铝毒的耐性表现。结果表明,大豆对铝毒的耐性存在基因型差异。首先通过简单钙培养法筛选得到耐酸铝材料12份,之后通过全营养法进行验证获得耐酸铝大豆种质资源8份,即沅陵矮子早<甲>、长沙夏黄豆、常德中和青豆、凤凰青皮豆<乙>、十月青豆、人潮溪黄豆1、中作04563、中作05675。进一步以垦丰15、桂春8号为对照品种,采用土培法对获得的8份大豆耐性材料进行盆栽鉴定,以单株粒重、百粒重、叶绿素含量和株高作为耐铝毒性的评价指标,分析大豆对铝毒耐性的综合表现。遴选出1份耐性较强的材料,即人潮溪黄豆1,可供大豆耐铝毒性遗传育种研究利用。
- Abstract:
- A total of 139 soybean germplasm mainly originated from Hunan province were tested with the hydroponics methods of simple calcium solution culture and complete nutrient solution culture in seedling stage to screen out tolerant germplasm to aluminum toxicity taking main roots as tolerance indicator.The results showed that tolerance to aluminum toxicity varied with genotypes.Twelve soybean germplasm showed tolerance to aluminum toxicity after simple calcium solution culture.When further tested by complete nutrient solution culture,eight soybean germplasm including Yuanling’aizizao(a),Changshaxiahuangdou,Changdezhongheqingdou,Fenghuangqingpidou(b),Shiyueqingdou,Renchaoxihuangdou 1,Zhongzuo 04563 and Zhongzuo 05675 showed tolerance to aluminum toxicity.Then,the eight soybean germplasm were pot cultured to compare their seed weight per plant,100-seed weight,chlorophyll content and plant height with Kenfeng 15 and Guichun 8 as control.Renchaoxihuangdou 1 showed stronger tolerance to Al toxicity than other materials and could be used in breeding soybean varieties tolerant to Al toxicity.
参考文献/References:
[1]Foy C D,Chaney R L,White M C.The physiology of metal toxicity in plants[J].Annual Review of Plant Physiology,1978,29:5l1-566.
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备注/Memo
基金项目:公益性行业(农业)科研专项(200903002);广东省科技计划项目(2011A020102010);广东省教育部产学研结合计划项目(2011B090400328);农业部公益性行业科研专项(nyhyzx07-004-11);现代农业产业技术体系建设专项(CARS-04-PS09);华南农业大学校长基金(4100-k09130)。