[1]黄鹤　赵月春　郭秀兰.大豆在铝胁迫下分泌的柠檬酸与品种的耐酸铝性无关[J].大豆科学,2003,22(03):218-222.[doi:10.11861/j.issn.1000-9841.2003.03.0218]
　Huang He Zhao Yuechun Guo Xiulan.ALUMINUM-INDUCED CITRATE EXUDATION SHOWS NOPOSITIVE RELATION WITH AL TOLERANCE IN SOYBEAN[J].Soybean Science,2003,22(03):218-222.[doi:10.11861/j.issn.1000-9841.2003.03.0218]
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大豆在铝胁迫下分泌的柠檬酸与品种的耐酸铝性无关

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年03期 页码: 218-222 栏目: 出版日期: 2003-08-25
Title:
ALUMINUM-INDUCED CITRATE EXUDATION SHOWS NOPOSITIVE RELATION WITH AL TOLERANCE IN SOYBEAN
文章编号:
1000 -9841(2003)03 -0218 -05
作者:
黄鹤　赵月春　郭秀兰
华南农业大学理学院, 广州510642
Author(s):
Huang He 　Zhao Yuechun 　Guo Xiulan
Col lege of Sciences , South China Agricultural University , Guangzhou , 510642
关键词:
大豆; 铝毒; 柠檬酸
Keywords:
Soybean ; Al tolerance ; Cit rate exudation
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2003.03.0218
文献标志码:
A
摘要:
试验采用8 个耐酸铝明显不同的大豆品种, 研究了在铝胁迫下大豆有机酸分泌及其与耐性的关系。同时也比较了大豆铝敏感品种LJ 和小麦耐铝品种At las 在铝胁迫下有机酸分泌的差异。大豆幼苗在含10 μM AlCl3的0 .5 mM CaCl2溶液中进行耐酸铝性筛选。结果表明, 大豆品种在耐酸铝方面存在明显的不同。大豆在50μM AlCl3 铝胁迫下可以分泌大量的柠檬酸, 但柠檬酸分泌量与大豆的耐酸铝性没有正相关性。经过铝处理一天的大豆, 恢复一天后再用铝处理, 多数品种分泌的柠檬酸明显减少, 特别是敏感品种柠檬酸分泌大幅减少, 但柠檬酸分泌量与耐酸铝性也没有相关性。进一步试验表明, 大豆铝敏感品种LJ 分泌的柠檬酸明显高于小麦耐性品种Atlas 分泌的有机酸总量(柠檬酸和苹果酸)。另外, 大豆主根的铝积累与耐铝性及柠檬酸分泌量没有相关性。因此, 试验结果表明, 铝诱导的有机酸分泌量及根尖的铝含量不能作为筛选大豆耐酸铝种质的筛选指标.
Abstract:
The varietal differences in Al -induced ci trate exudation and i ts relation to Al tolerance w ere investigated , and a comparative study of differences in organic acid secretion between Al -sensitive soybean LJ and Altolerant w heat Atlas was conducted .Soybeans were exposed to the 0 .5 mM Ca solution containing 0 and 10μM AlCl3 to identify differences in Al tolerance .Based on relative root elongation (%of control), soybeans exhibiteda range of difference in Al tolerance .Cit rate secretion under 50 mM AlCl3(24 h)st ress dif fered amongthe 8 varieties used , but no relation betw een the Al tolerance and Al -induced citrate secretion was observed .Al-induced ci trate secret ions from most of the varieties w as g reatly reduced by pre -t reatment w ith 50 AlCl3 mMfor 24 h .Sensitive soybean LJ exuded more org anic acid (ci trate only)than did Al -tolerant w heat Atlas,w hich secreted bo th cit rate and malate .Al accumulation in roo t tips w as not related to Al tolerance and Al -inducedcit rate secretion .Thus , this experiment suggests that Al -induced citrate exudation and Al accumulat ionin root tips can not be used as standards fo r screening Al -tolerant soybeans .

参考文献/References:

1 　Matsumoto , H .Cell biology of aluminium t oxici ty and t olerance inhigher plant s .Int Rev Cyt ol , 2000 , 200 :1 -46.

2 　Ma, J .F ., Zheng , S .J ., Mat sum ot o, H .Detoxifying aluminiumw ith buckw heat [ J] .Natu re , 1997 , 390 :569 -570.
3 　Delhaize , E ., Ryan , P.R ., Randall , P .J .Aluminium tolerancein w heat [ Tr it icum aesti vum L .] II .Aluminium -st imulated excretionof malic acid f rom root apices [ J] .Plant Physiol, 1993 ,103 :695 -702.
4 　Pellet , D .M ., Grunes , D.L ., Kochian , L .V .Organi c acid exudationas an aluminium -tolerance mechanism in maize (Zea maysL .)[ J] .Plant a, 1995 , 196 :788 -795.
5 　Ma, J , F, , Taketa , S .Yang , Z ., M .Aluminium tolerance geneson the short arm of chromosome 3R are linked to organi c acid releasein triti cale [ J] .Plan t physiol , 2000 , 122 :687 -694.
6 　Yang , Z , M., Sivaguru , M .Hors t , W.J ., et al.Aluminium t oleranceis achieved by exudat ion of ci tric acid f rom root s of soybean (Glycine max L.Merr .)[ J] .Physiol Plant , 2000 , 110 :72 -77.
7 　Ma, Z., Miyasaka, S .C .Oxalat e exudation by taro in response t oAl[ J] .Plant Physiol , 1998 , 118 :861 -865.
8 　Ishikaw a, Wagatsuma, S ., T ., Sasaki , R., et al.Comparison ofthe amount of cit ric and Malic acids in Al media of seven plantspecies and tw o cultivars each in five plant species[ J] .S oil S ci.PlantNutr , 2000 , 46(3):751 -758 .
9 　Yang , Z .M ., Nian , H ., S ivagu ru , M ., et al.Charact erizat ion ofaluminium - induced ci trat e secret ion in aluminium -tolerant soybean(Glycine ma x L .)plants[ J] .Physiol.Plant , 2001 , 113 (1).
10 Nian , H ., Yang , Z .M ., Ahn , S .J ., et al.A comparat ive study onaluminum - and copper -induced organi c acids f rom w heat root s[ J] .Physiol.Plant , 2002, 116 (3):328-335.
11 Delhaize E , Ryan , P .R., Randall , P .J .Aluminum tolerance inw heat (T rit icum aestivum L.) I .Uptake and dist ribut ion of Aluminumin root apices[ J] .Plant Physiol , 1993 , 103 :685 -693.
12 Zhang , G ., Taylor, G.T .Ef fect of aluminum on grow th and di stribution of aluminum in tolerant and sensitive cultivars of triticumaestivum L [ J] .Commun Soil Sci.Plan t Anal 1988 , 19 :1195 -1205.
13 Bianchi-Hall, C .M ., Carter , T .E ., Ruft y , T .W ., et al.Heritability and resource allocation of aluminum tolerance derived fromsoybean PI 416937 [ J] .Crop Sci.1998 , 38 :513 -522.
14 Nian , H ., Ahn , S .J ., Yang, Z .M ., et al.Ef fect of phosphorusdefi ciency on aluminum - induced cit rate exudat ion in soybean(Gl ysine , Max .L.Merr .)[ J] .Physiol.Plant , 2003 , 117 (1).
15 Si lva , I .R., Smyth , T .J ., Israel , D.W., et al.Magnesium ameliorates aluminum rhizot oxici ty in soybean by increasing cit ric acidproduct ion and exudation by root s [ J] .Plant and Cell Physiol.2001 , 42 :546 -554.
16 年海,卢永根.大豆耐酸铝种质筛选概况[ J] .华南理工大学学报1996 , 24 :(自然科学版, 增刊)138 -143.

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备注/Memo

基金项目:国家自然科学基金资助项目(批准号:3970086);农业部948 资助项目(991023)　

作者简介:黄鹤(1960 -), 女, 实验师, 主要从事大豆耐酸铝及低磷机理研究。

更新日期/Last Update: 2016-12-31