YAN Fan,SUN Xin,ZHAI Ying,et al.Effect of Different 6-BA Concentration and Genotypes on Shoots Induced from Embryonic Tips[J].Soybean Science,2011,30(01):29-32.[doi:10.11861/j.issn.1000-9841.2011.01.0029]
6-BA浓度及基因型对大豆胚尖诱导丛生芽的影响
- Title:
- Effect of Different 6-BA Concentration and Genotypes on Shoots Induced from Embryonic Tips
- 文章编号:
- 1000-9841（2011）01-0029-04
- Keywords:
- Soybean; Embryonic tip; 6-BA; Genotype
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以吉林35、吉林47等5个大豆品种为材料，胚尖为外植体，研究不同浓度6-BA对丛生芽诱芽率和平均芽数的影响，同时测定吉林35在胚尖诱导丛生芽的过程中对潮霉素的耐受性。结果表明：3.0 mg·L-1 6-BA浓度下吉林35的诱芽率最高，综合考虑丛生芽诱芽率和平均芽数2个性状，吉林35更加适合于大豆胚尖再生系统；在遗传转化中，以3.0 mg·L-1潮霉素作为吉林35抗性筛选的适宜浓度。
- Abstract:
- In order to establish an efficient embryonic tip regeneration system for soybean (Glycine max L.Merr.), five soybean cultivars, including Jilin 35, were used as explants to study the effects of 6-BA and genotypes on the numbers and induction rate of adventitious shoots. The endurance of the induced shoots of Jilin 35 to Hygromycin B was also studied. The results showed that Jilin 35 was fit for soybean embryonic tip regeneration system according to the numbers and inductivity of adventitious shoots. The concentration of 3.0 mg·L-1 Hygromycin B was advised for resistance screening in embryonic tip regeneration system.
参考文献/References:
[1]邱承祥, 武天龙. 6- BA对大豆茎尖诱导再生植株的研究[J]. 大豆科学, 2003, 22(1):32-35. (Qiu C X, Wu T L. Study on 6-BA to the regeneration of tip shoot of soybean[J]. Soybean Science, 2003, 22(1):32-35.) [2]张东旭, 张洁, 商蕾, 等. 大豆胚尖再生体系的研究[J]. 河北农业大学学报, 2008, 30(4):7-13. (Zhang D X, Zhang J, Shang L, et al. Study on the regeneration system of soybean embryonic tips[J]. Journal of Hebei Agricultural University, 2008, 30(4):7-13.) [3]刘海坤, 卫志明. 利用根癌农杆菌介导转化大豆成熟种子胚尖获得转基内植株[J]. 植物生理与分子生物学学报, 2004, 30(6):631-636. (Liu H K, Wei Z M. Transgenic soybean obtained with agrobacterium tumfaciens-mediated transformation of embryonic tip of soybean mature seeds[J]. Journal of Plant Physiology and Molecular Biology, 2004, 30(6):631-636.) [4]林树柱, 曹越平, 卫志明, 等. 6- BA诱导大豆子叶节和茎尖出芽的研究[J]. 上海交通大学学报 (农业科学版), 2005, 23 (2):138-142. (Lin S Z, Cao Y P, Wei Z M, et al. Studies on shoots induced by 6-BA from cotyledonary nodes and embryonic tips of soybean[J].Journal of Shanghai Jiaotong University (Agricultural Science Edition), 2005, 23(2):138-142. ) [5]李海燕, 武小霞, 刘森, 等. 大豆子叶节、胚尖再生植株的研究[J]. 大豆科学, 2007, 26(5):709-712. (Li H Y, Wu X X, Liu S, et al. Plant regeneration from cotyledonary nodes and embryonic tips of soybean[J]. Soybean Science, 2007, 26(5):709-712.) [6]孙文丽, 刘昱辉, 吴元华, 等. 大豆胚芽尖再生体系的建立及转基因初步研究[J]. 湖北农业科学, 2008, 47(6):615-618. (Sun W L, Liu Y H, Wu Y H, et al. A pilot study of an soybean embryonic tip regeneration system[J]. Hubei Agricultural Sciences, 2008,47(6):615- 618.) [7]刘海坤, 卫志明. 大豆遗传转化研究进展[ J ]. 植物生理与生物学报, 2005, 31(2):126-134. (Liu H K, Wei Z M. Recent advances in soybean genetic transformation[J]. Journal of Plant Physiology and Molecular Biology, 2005, 31(2):126-134.) [8]王萍, 张淑珍, 李文滨, 等. 大豆不同基因型胚尖不定芽的诱导及对抗生素的敏感性[J]. 作物杂志, 2010(2):50-53. (Wang P, Zhang S Z, Li W B, et al. Induction of adventitious shoots from embryonic tip of different soybean genotype and their sensibility to antibiotics[J]. Crops, 2010(2):50-53.) [9]邹莉, 文艺, 张匀华, 等. 大豆子叶节对潮霉素敏感性研究[J]. 大豆科学, 2008, 27(2):267-269. (Zou L, Wen Y, Zhang Y H, et al. Susceptibility of soybean cotyledonary node to hygromycin B[J]. Soybean Science, 2008, 27(2):267-269.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]张忻爽,王萍,宋海星,等.卡那霉素和草铵膦对不同基因型大豆胚尖不定芽诱导的影响[J].大豆科学,2013,32(01):136.[doi:10.3969/j.issn.1000-9841.2013.01.032]
ZHANG Xin-shuang,WANG Ping,SONG Hai-xing,et al.Effect of Knamycin and Glufosinate on Adventitious Buds Induction from Embryonic Tip of Different Genotype Soybean[J].Soybean Science,2013,32(01):136.[doi:10.3969/j.issn.1000-9841.2013.01.032]
[12]张兴政,王昌陵,卢福荣,等.根癌农杆菌介导大豆转化 LePT1 基因的研究[J].大豆科学,2014,33(01):31.[doi:10.11861/j.issn.1000-9841.2014.01.0031]
ZHANG Xingzheng,WANG Changling,LU Furong,et al.Transformation of LePT1 Gene into Soybean via Agrobacterium mediation[J].Soybean Science,2014,33(01):31.[doi:10.11861/j.issn.1000-9841.2014.01.0031]
[13]钟 影,王 罡,季 静,等.草甘膦对不同基因型大豆不定芽再生的影响[J].大豆科学,2014,33(02):218.[doi:10.11861/j.issn.1000-9841.2014.02.0218]
ZHONG Ying,WANG Gang,JI Jing,et al.Effect of Glyphosate on Adventitious Buds Induction from Embryonic Tip of Different Genotype Soybean[J].Soybean Science,2014,33(01):218.[doi:10.11861/j.issn.1000-9841.2014.02.0218]
[14]闫帆,孙昕,翟莹,等.大豆胚尖再生体系的研究[J].大豆科学,2011,30(05):757.[doi:10.11861/j.issn.1000-9841.2011.05.0757]
YAN Fan,SUN Xin,ZHAI Ying,et al.Optimization on the Regeneration System of Soybean Embryonic Tips[J].Soybean Science,2011,30(01):757.[doi:10.11861/j.issn.1000-9841.2011.05.0757]
[15]郑丽红,季 静,王 罡,等.适于子叶节和胚尖再生体系的大豆基因型筛选[J].大豆科学,2012,31(02):212.[doi:10.3969/j.issn.1000-9841.2012.02.011]
ZHENG Li-hong,JI Jing,WANG Gang,et al.Selection of Suitable Soybean Genotype Based on Cotyledon Node and Embryonic Tip Regeneration Systems[J].Soybean Science,2012,31(01):212.[doi:10.3969/j.issn.1000-9841.2012.02.011]
[16]王 伟,王 罡,季 静,等.大豆胚尖再生体系的优化及与子叶节再生体系的比较[J].大豆科学,2012,31(03):353.[doi:10.3969/j.issn.1000-9841.2012.03.004]
WANG Wei,WANG Gang,JI Jing,et al.Optimization of Embryonic Tip Regeneration System and Comparison with Cotyledonary Node Regeneration System in Soybean[J].Soybean Science,2012,31(01):353.[doi:10.3969/j.issn.1000-9841.2012.03.004]
[17]姚丙晨,沈艳茹,韩 雪,等.大豆子叶节和胚尖再生体系的比较及大豆SR1基因的遗传转化[J].大豆科学,2012,31(03):364.[doi:10.3969/j.issn.1000-9841.2012.03.006]
YAO Bing-chen,SHEN Yan-ru,HAN Xue,et al.Comparison with Cotyledonary Node and Embryonic Tip Regeneration System in Soybean\[Glycine max(L.)Merrill\] and Genetic Transformation of SR1 [J].Soybean Science,2012,31(01):364.[doi:10.3969/j.issn.1000-9841.2012.03.006]
[18]许 诺,张 君,王丕武.6-BA和IBA浓度对不同基因型大豆胚尖诱导丛生芽的影响[J].大豆科学,2012,31(04):678.[doi:10.3969/j.issn.1000-9841.2012.04.033]
XU Nuo,ZHANG Jun,WANG Pi-wu.Effect of 6-BA and IBA Concentration on Shoots Induction from Embryonic Tips of Four Soybean Genotypes[J].Soybean Science,2012,31(01):678.[doi:10.3969/j.issn.1000-9841.2012.04.033]
[19]朱红林,沙爱华,符秀梅,等.转录调控基因GmLEC1转化大豆及转化方法的比较[J].大豆科学,2010,29(01):7.[doi:10.11861/j.issn.1000-9841.2010.01.0007]
ZHU Hong-lin,SHA Ai-hua,FU Xiu-mei,et al.Cloning and Transformation Study of Transcription Factor GmLEC1 in Soybean[J].Soybean Science,2010,29(01):7.[doi:10.11861/j.issn.1000-9841.2010.01.0007]
[20]孙式静,杨素欣,冯献忠.超声波辅助处理农杆菌介导大豆胚尖转化转基因植株的获得和分子鉴定[J].大豆科学,2014,33(06):808.[doi:10.11861/j.issn.1000-9841.2014.06.0808]
SUN Shi-jing,YANG Su-xin,FENG Xian-zhong.Molecular Characterization of the Transgenic Soybean Plants by Sonication-assisted Agrobacterium-mediated Transformation Using the Embryonic Tips[J].Soybean Science,2014,33(01):808.[doi:10.11861/j.issn.1000-9841.2014.06.0808]
备注/Memo
基金项目：转基因生物新品种培育重大专项资助项目（2008ZX08004-003）；国家自然科学基金面上资助项目（30971808）；吉林省科技发展计划重点资助项目（20080204）；长春市科技局国际科技合作资助项目（08GH10）；“211工程”三期重点学科建设资助项目。