[1]王 慧,高中杰,范 蕊,等.基于三种抗性机制评价大豆对抗斜纹夜蛾的抗性[J].大豆科学,2011,30(01):8-14.[doi:10.11861/j.issn.1000-9841.2011.01.0008]
　WANG Hui,GAO Zhong-jie,FAN Rui,et al.Evaluation of Resistance of Soybean Germplasm to Common Cutworm Based on Three Resistance Mechanisms[J].Soybean Science,2011,30(01):8-14.[doi:10.11861/j.issn.1000-9841.2011.01.0008]
点击复制

基于三种抗性机制评价大豆对抗斜纹夜蛾的抗性

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年01期 页码: 8-14 栏目: 出版日期: 2011-02-25
Title:
Evaluation of Resistance of Soybean Germplasm to Common Cutworm Based on Three Resistance Mechanisms
文章编号:
1000-9841（2011）01-0008-07
作者:
王 慧; 高中杰; 范 蕊; 章银珊; 吴 倩; 喻德跃
南京农业大学 大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室，江苏 南京 210095
Author(s):
WANG Hui;  GAO Zhong-jie;  FAN Rui;  ZHANG Yin-shan;  WU Qian;  YU De-yue
National Key Laboratory of Crop Genetics and Germplasm Enhancement； National Center for Soybean Improvement, Ministry of Agriculture; Soybean Research Institute, Nanjing Agricultural University，Nanjing 210095, Jiangsu,China
关键词:
大豆; 斜纹夜蛾; 抗生性; 抗选性; 耐害性
Keywords:
Soybean;  Common cutworm;  Antibiosis;  Antixenosis;  Tolerance
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.01.0008
文献标志码:
A
摘要:
通过室内养虫鉴定，农艺性状考查和收获后单株考种，对194份栽培大豆进行了抗斜纹夜蛾的鉴定和评价。结果表明：在抗生性和抗选性上对斜纹夜蛾表现为高抗或抗的材料分别有77份和107份。194份品种在株高、主茎分枝数、单株粒数和单株粒重性状上表现出不同程度的耐害和补偿能力。大豆对斜纹夜蛾的抗生性与抗选性的相关性表现相对一致，而抗生性或抗选性与耐害性的表现无显著相关。在抗性鉴定的基础上，筛选出一批对斜纹夜蛾在3种抗性机制上均表现优异的大豆材料25份，为大豆抗虫遗传和育种提供新的抗源。
Abstract:
The common cutworm is a major pest of soybean in the southern China. In this study, the insect bioassay was applied to evaluate the resistance of 194 soybean cultivars. Agronomic traits were measured after the leaf-feeding test and sampled at V6 growth stage and after the harvest， respectively. In the insect bioassay, there were 77 resistant soybean cultivars based on the antibiosis and 107 resistant soybean cultivars based on the antixenosis. In the tolerance test, the 194 soybean cultivars performed different tolerance. The correlation analysis was used to analyze the relationship of the three resistance mechanisms. The result showed that the antibiosis had extremely significant positive correlation with the antixenosis. However, neither the antibiosis nor antixenosis had significant positive correlation with the tolerance. Based on the resistant test, twenty-five elite soybean resistance germplasms were selected, which could be used as new resistant sources in soybean genetics and breeding.

参考文献/References:

[1]贝亚维，茹水江，陈笑芸，等. 温度对斜纹夜蛾生长发育和存活的影响[J]. 浙江农业学报，2001，13(4):197-200.（Bei Y W, Ru S J, Chen X Y, et al. The effect of temperature on the development and survival of Spodetera litura，Fabricius[J]. Acta Agriculturae Zhejiangensis，2001，13(4) :197-200.）

[2]崔章林，盖钧镒，吉东风，等. 南京地区大豆食叶性害虫种类调查与分析[J]. 大豆科学，1997，16（1）：12-20. (Cui Z L, Gai J Y, Ji D F, et al. A study on leaf-feeding insect species on soybean in Nanjing area[J]. Soybean Science, 1997, 16 (1):12-20.)

[3]Rector B G, All J N, Parrott W A,et al. Identification of molecular markers linked to quantitative trait loci for soybean resistance to corn earworm[J]. Theoretical and Applied Genetics, 1998, 96:786-790.

[4]Narvel J M, Walker D R, Rector B G, et al. A retrospective DNA marker assessment of the development of insect resistant soybean[J]. Crop Science, 2001, 41：1931-1939.

[5]周明牂. 作物抗虫性原理及应用[M]. 北京：北京农业大学出版社，1992：10-12. (Zhou M Z. Crops insect-resistance principle and application [M]. Beijing：Beijing Agricultural University Press, 1992：10-12.)

[6]Rector B G, All J N, Parrott W A, et al. Quantitative trait loci for antixenosis resistance to corn earworm in soybean[J]. Crop Science, 1999, 39：531-538.

[7]Rector B G, All J N, Parrott W A, et al. Quantitative trait loci for antibiosis resistance to corn earworm in soybean[J]. Crop Science, 2000, 40：233-238.

[8]Terry L I, Chase K, Jarvik T, et al. Soybean quantitative trait loci for resistance to insects[J]. Crop Science, 2000, 40：375-382.

[9]Zhu S, Walker D R, Boerma H R, et al. Fine mapping of a major insect resistance QTL in soybean and its interaction with minor resistance QTLs[J]. Crop Science， 2006, 46：1094-1099.

[10]孙祖东，盖钧镒. 大豆对食叶性害虫抗性机制的研究[J]. 中国油料作物学报, 1999, 21(2)：60-64. (Sun Z D, Gai J Y. The mechanism of resistance of soybeans to leaf-feeding insects[J]. Chinese Journal of Oil Crop Sciences, 1999, 21(2)：60-64.)

[11]段灿星, 张世贤, 陈青, 等. 水稻种质资源抗灰飞虱评价及抗性机制分析[J]. 中国水稻科学, 2007, 21(4)：425-430. (Duan C X, Zhang S X, Chen Q, et al. Evaluation of rice germplasm for resistance to the small brown plant hopper and analysis on resistance mechanism[J]. Chinese Journal of Rice Science, 2007, 21(4)：425-430.)

[12]赵利敏, 杜晓莉, 贾豪, 等. 灰翅麦茎蜂对不同品种春小麦穗粒重的影响[J]. 植物保护, 1997, 23(6)：15-16. (Zhao L M, Du X L, Jia H, et al. Effect of Cephus fumipennis eversmannon grain weight of different spring wheat varieties[J]. Plant Protection, 1997, 23(6)：15-16.)

[13]胡桂馨, 师尚礼, 王森山, 等. 不同苜蓿品种对牛角花齿蓟马的耐害性研究[J]. 草地学报，2009，17（4）：505-509. (Hu G X, Shi S L, Wang S S, et al. Tolerance of alfalfa varieties to Odontothrips loti Haliday[J]. Acta Agrestia Sinica, 2009, 17(4)：505-509.)

[14]陈建明, 俞晓平, 程家安, 等. 植物耐虫性研究进展[J].昆虫学报，2005，48（2）：262-272. (Chen J M，Yu X P，Chen J A， et al. Plant tolerance against insect pests and its mechanisms[J]. Acta Entomologica Sinica，2005，48（2）：262-272.)

[15]盖钧镒，崔章林. 大豆抗食叶性害虫育种的鉴定方法与标准[J]. 作物学报，1997, 23(4)：400-407. (Gai J Y, Cui Z L. A study on methods and criteria of identification of resistance to leaf-feeding insects in soybean breeding[J]. Acta Agronomica Sinica, 1997, 23(4)：400-407.)

[16]Van Duyu J W, Turnipseed S G, Maxwell J D. Resistance in soybeans to the Mexican bean beetle. Ⅰ.Sources of resistance[J]. Crop Science，1971, 11：572-573.

[17]Van Duyu J W, Turnipseed S G, Maxwell J D. Resistance in soybeans to the Mexican bean beetle. Ⅱ.Reaction of the beetle to resistant plants[J]. Crop Science，1972, 12：561-562.

[18]Hatchett J H, Beland G L, Hartwig E E. Leaf-feeding resistance to bollworm and tobacco budworm in three soybean plant introductions[J]. Crop Science, 1976, 16：277-280.

[19]崔章林，盖钧镒. 大豆抗食叶性害虫的研究进展[J]. 大豆科学，1996，15（2）：149-158. (Cui Zh L, Gai J Y. Advance of study on soybean leaf-feeding insects[J]. Soybean Science, 1996, 15(2)：149-158.)

[20]吴巧娟，吴娟娟，吴业春，等. 大豆资源对斜纹夜蛾的抗性鉴定[J]. 大豆科学，2006，25（4）：410-409. (Wu Q J, Wu J J, Wu Y C, et al. Evaluation of resistance of soybean germplasm to cotton worm (Prodenial litura, Fabricius)[J]. Soybean Science, 2006, 25(4)：409-413.)

[21]吴业春, 王慧, 吴巧娟, 等. 大豆对食叶性害虫田间抗性的相对稳定性[J]. 中国油料作物学报，2004，26（4）：66-70. (Wu Y C, Wang H, Wu Q J,et al. Relative stablity on resistance of soybeans to leaf-feeding instcts in the field[J]. Chinese Journal of Oil Crop Sciences, 2004, 26(4)：66-70.)

[22]王慧，喻德跃，吴巧娟，等. 大豆对斜纹夜蛾抗生性基因的微卫星标记(SSR)的研究[J]. 大豆科学，2004，23（2）：91-95. (Wang H, Yu D Y, Wu Q J,et al. Characterization of resistance genes to cotton worm with SSR markers in soybean[J]. Soybean Science, 2004, 23(2)：91-95.)

[23]Komatsu K, Okuda S, Takahashi M, et al. QTL mapping of antibiosis resistance to common cutworm (Spodoptera litura Fabricius) in soybean[J]. Crop Science, 2005, 45：2044-2048.

[24]刘华，王慧，李群，等. 大豆对斜纹夜蛾抗性的遗传分析及相关QTL的定位[J]. 中国农业科学，2005, 38(7)：1369-1372. (Liu H, Wang H, Li Q, et al. Inheritance analysis and mapping QTLs related to cotton worm resistance in soybean[J]. Scientia Agricultura Sinica, 2005, 38(7)：1369-1372. )

[25]付三雄，王慧，吴娟娟，等. 应用重组自交系群体定位大豆抗虫QTL[J]. 遗传，2007, 29(9)：1139-1143. (Fu S X, Wang H, Wu J J, et al. Mapping insect resistance QTLs of soybean with RIL population[J]. Hereditas, 2007, 29(9)：1139-1143.)

[26]詹秋文，盖钧镒. 大豆种质资源对斜纹夜蛾(Prodenia Litura)抗性的鉴定[J]. 应用与环境生物学报，2000,6(1):18-23. (Zhan Q W，Gai J Y. Evaluation of resistance of soybean germplasm to cotton worm (Prodenia litura, Fabricius)[J]. Chinese Journal of Applied & Environmental Biology, 2000, 6(1)：18-23.)


相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]胡壮壮,徐先超,潘霖,等.不同生殖生长期大豆器官对斜纹夜蛾抗性分析[J].大豆科学,2020,39(06):932.[doi:10.11861/j.issn.1000-9841.2020.06.0932]
　HU Zhuang-zhuang,XU Xian-chao,PAN Lin,et al.Resistance Analyses of Soybean Organs to Common Cutworm (Spodoptera litura) at Different Reproductive Stages[J].Soybean Science,2020,39(01):932.[doi:10.11861/j.issn.1000-9841.2020.06.0932]

备注/Memo

基金项目：国家重点基础研究发展计划资助项目（2010CB125906）；南京农业大学青年科技创新基金资助项目（KJ09004）。

第一作者简介：王慧（1977-），女，讲师，硕士，主要从事大豆抗虫遗传育种。 E-mail：wanghui0@njau.edu.cn。
通讯作者：喻德跃（1965-），男，教授，博士生导师，主要从事作物遗传育种研究。E-mail：dyyu@njau.edu.cn。

更新日期/Last Update: 2014-09-11