[1]吴伟,等.栽培大豆端粒相关序列的克隆及定位[J].大豆科学,2010,29(03):380-384.[doi:10.11861/j.issn.1000-9841.2010.03.0380]
　WU Wei,LI Hong-jie,et al.Cloning and Mapping of Telomere Associated Sequence in Soybean[J].Soybean Science,2010,29(03):380-384.[doi:10.11861/j.issn.1000-9841.2010.03.0380]
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栽培大豆端粒相关序列的克隆及定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年03期 页码: 380-384 栏目: 出版日期: 2010-06-25
Title:
Cloning and Mapping of Telomere Associated Sequence in Soybean
文章编号:
1000-9841（2010）03-0380-05
作者:
吴伟1;  2; 李洪杰2; 王晓鸣2; 朱振东2; 东方阳1
1.河北科技师范学院 生命科学实验中心，河北 昌黎 066600；
2.中国农业科学院 作物科学研究所,国家农作物基因资源与基因改良重大科学工程，北京 100081
Author(s):
WU Wei1;  2;  LI Hong-jie2;  WANG Xiao-ming2;  ZHU Zhen-dong2;  DONGFANG-Yang1
1. Academic center of Life Science, Hebei Normal University of Science and Technology, Changli 066600, Hebei；
2. Institute of Crop Science, Chinese Academy of Agricultural Sciences,National Key Facility of Crop Gene Resource and Genetic Improvement, Beijing 100081,China
关键词:
栽培大豆; 端粒相关序列; 重复单元; 克隆; 定位
Keywords:
Soybean cultivar; Telomere associated sequence; Telomeric repeat motif; Clone; Mapping
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.03.0380
文献标志码:
A
摘要:
以拟南芥的端粒重复序列(TR)为引物(TTTAGGG)3，在栽培大豆中扩增并克隆了1个574 bp的DNA片段。序列分析表明：该片段与大豆端粒相关序列的相似度高达92％～99％，与白玉草TR-TAS的间隔区的相似度为79%，与大麦和玉米等其它植物的TAS的相似度在16％～35％之间；这一片段含有14个拷贝的拟南芥类型端粒重复单元，并且还有30个重复单元发生了碱基突变(缺失、替换与插入)。该端粒相关序列具有1个25 bp的保守重复单元，串联重复13个拷贝，且该序列的A+T含量高于60%，体现了卫星DNA的特征。该序列被定位在大豆3号染色体的近末端。
Abstract:
Using the Arabidopsis-type telomeric repeat (TTTAGGG)3 as a primer, a 574 bp TAS was amplified and cloned from three soybean (Glycine max) cultivars. Sequence analysis indicated that the similarities of the TASs from different cultivars in this study and other TASs from GenBank ranged from 92% to 99%. A similarity of 79% was detected between the TAS obtained and the interval sequence of TR-TAS from Silene latifolia. Low similarities ranging from 16% to 35% were observed between the soybean TAS and other plants such as barley (Hordeum vulgare) and corn (Zea mays). In this TAS fragment, there were 14 copies of Arabidopsis-type telomeric repeat (TTTAGGG/CCCTAAA) and 30 copies of mutation motifs with deletion, substitution and inversion. The 25 bp conserved tandem repeat in this TAS fragment occurred in 13 copies and the content of adenine and thymine (A+T) was greater than 60%, showing the characterization of satellite DNA. This TAS fragment was mapped to the proximal end of soybean chromosome 3.

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备注/Memo

基金项目：河北省自然科学基金资助项目(303408)。

第一作者简介：吴伟（1984-），男，在读硕士，研究方向为植物分子生物学。
通讯作者：东方阳，博士，教授。E-mail：yang_dongfang@hotmail.com。

更新日期/Last Update: 2014-09-13