XU Peng-fei,WU Jun-jiang,FAN Su-jie,et al.Isolation of Phytophthora sojae and Identification of Resistance to Race 3 in Soybean Germplasm[J].Soybean Science,2010,29(02):272-275.[doi:10.11861/j.issn.1000-9841.2010.02.0272]
大豆疫霉根腐病菌的分离鉴定及种质资源对3号生理小种的抗性评价
- Title:
- Isolation of Phytophthora sojae and Identification of Resistance to Race 3 in Soybean Germplasm
- 文章编号:
- 1000-9841（2010）02-0272-04
- Keywords:
- Soybean germplasm; Phtophthora sojae; Race 3; Germplasm screening
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用下胚轴伤口接种法，用在黑龙江省建三江农场分离到的大豆疫霉菌3号生理小种对292份栽培大豆材料（其中农家品种153份、其它大豆栽培品种139份）和236份野生大豆材料进行了抗性鉴定。结果表明：栽培大豆资源抗病80份，占27.4%，中间类型93份，占31.8%，感病119份，占40.8%。153份农家品种中，抗病的有49份，占农家品种的32.0%，表明农家大豆品种资源抗性比例较高。野生大豆资源中抗病的有49份，占20.8%；中间类型55份，占23.3%；感病132份，占55.9%。鉴定的这些高抗资源可为我国大豆抗疫霉根腐病育种奠定基础。
- Abstract:
- Phytophthora root and stem rot of soybean caused by Phytophthora sojae is a destructive disease in soybean production regions all around the world. Utilization resistant cultivar is the most economical and environmentally safe method for preventing against the disease, and screening on resistance germplasm is the basement for resistance breeding. A total of 292 soybean cultivars (including 153 native soybean germplasm and 139 soybean cultivars) and 236 wild soybean germplasm were inoculated with race 3 of Phtophthora sojae isolated from Jiansanjiang where there is serious disease occurring in Heilongjiang Province. A total of 80 cultivars were resistant, 93 had intermediate reaction, and 119 were susceptible to race 3, representing 27.4%, 31.8% and 40.8% of all the cultivars tested respectively. The number of resistant native soybean was 49, representing 32.0% of all the native soybean germplasm, which means that the percentage of native resistant germplasm was higher. A total of 49 wild soybean germplasm were resistant, 55 had intermediate reaction, and 132 were susceptible to race 3, representing 20.8%, 23.3%, and 55.9% of all the wild soybean germplasm tested, respectively. These resistant germplasm can be used in breeding for resistance to P. sojae.
参考文献/References:
[1]Schmitthenner A F. Problems and progress in control of phytophthora root rot of soybean[J]. Plant Disease, 1985, 69: 362-368. [2]Hildebrand A A. A root and stalk rot caused by P. me.var.?sojae[J]. Canadian Journal of Botany, 1959, 37: 927-957. [3]Hilty J W. Phytopathogenic and cultural variability of single zoospore isolates of P. me.?var. sojae[J].Phytopathology, 1962, 52: 859-862. [4]Jee H, Kim W, Cho W. Occurrence of Phytophthora root rot on soybean (Glycine max)and identification of the causal fungus[J]. Crop Protection, 1998, 40: 16-22. [5]沈崇尧, 苏彦纯. 中国大豆疫霉菌的发现及初步研究[J]. 植物病理学报, 1991, 21(3): 298. (Shen C Y, Su Y C. Discovery and preliminaty studies of Phytophthora megaspermaon soybean in China[J].Acta Phytopathologica Sinica, 1991, 21(3): 298.) [6]韩晓增, 何志鸿, 张增敏. 大豆主要病虫害防治技术[J]. 大豆通报, 1998, (6): 5-6. (Han X Z, He Z H, Zhang Z M. Major pests prevention in soybean[J]. Soybean Bulletin, 1998(6): 5-6.) [7]Leiz R A, Harman G L. Races of Phytophthora sojaeon soybean in Illinois[J]. Plant Disease, 2000, 84: 487. [8]许修宏, 吕慧颖, 杨庆凯, 等. 大豆疫霉根腐病抗源筛选[J]. 大豆科学, 1999, 18(2): 147-150. (Xu X H, Lu H Y, Yang Q K, et al.Screening of soybean germplasms for resistance to Phytophthora sojae[J]. Soybean Science, 1999, 18(2): 147-150.) [9]朱振东, 王晓鸣, 常汝镇, 等. 黑龙江省大豆疫霉菌生理小种鉴定及大豆种质的抗性评价[J]. 中国农业科学, 2000, 33(1): 62-67. (Zhu Z D, Wang X M, Chang R Z, et al. (Identification of Race of Phytophthora sojaeand reaction of soybean germplasm resources in Heilongjiang Province[J]. Scientia Agricultura Sinica, 2000, 33(1): 62-67.) [10]王晓鸣, 朱振东, 王化波, 等. 大豆种质对疫霉根腐病抗性特点研究[J]. 植物遗传资源科学, 2001, 2(2): 22-26. (Wang X M, Zhu Z D, Wang H B, et al.The resistance of soybean germplasm to Phytophthora root rot[J]. Journal of Plant Genetic Resources, 2001, 2(2): 22-26.) [11]霍云龙, 朱振东, 李向华, 等. 抗大豆疫霉根腐病野生大豆资源的初步筛选[J]. 植物遗传资源学报, 2005, 6(2): 182-185. (Huo Y L, Zhu Z D, Li X H, et al. Preliminary screening for Phytophthora root rot resistance in wild soybean[J]. Journal of Plant Genetic Resources, 2005, 6(2): 182-185.) [12]靳立梅, 徐鹏飞, 吴俊江, 等. 野生大豆种质资源对大豆疫霉根腐病抗性评价[J]. 大豆科学, 2007, 26(3): 300-304. (Jin L M, Xu P F, Wu J J, et al. Identification the resistance of wild soybean germplasm to Phytophthora soja[J]. Soybean Science, 2007, 26(3): 300-304.) [13]张淑珍, 徐鹏飞, 吴俊江, 等. 栽培大豆种质资源对大豆疫霉根腐病的抗性评价[J]. 大豆科学, 2007, 26(6): 914-917. (Zhang S Z,Xu P F,Wu J J,et al.Identification of resistance to Phytophthora sojaein soybean germplasm[J].Soybean Science, 2007, 26(6): 914-917.) [14]方仲达. 植病研究方法(第三版)[M]. 北京: 农业出版社, 1998, 12-13.(Fang Z D. Plant disease sesearch methods. the third edition[M]. Beijing: Agriculture Press, 1998, 12-13.) [15]徐鹏飞. 大豆疫霉根腐病菌遗传多样性分析及种质资源抗性评价[D]. 哈尔滨：东北农业大学, 2007： 25-29. (Xu P F. The analysis of genetic diversity of Phytophthora sojaeand evaluation for resistan germplasm[D].Harbin：Northeast Agricultural University, 2007：25-29.) [16]左豫虎, 臧忠婧, 刘錫若,等. 影响大豆疫霉菌(Phytophthora sojae)游动孢子产生的条件[J]. 植物病理学报, 2001, 31(3): 241-245. (Zuo Y H, Zang Z J, Liu T R, et al. Studies on production condition of zoospores of Phytophthora soja[J]. Acta Phytopathologica Sinica, 2001, 31(3): 241-245.) [17]Schmitthenner A F. Phytophthora rot [M]//In “Compendium of soybean disease.”3rd ed.J B Sinclair，P A Backman. American Phytophthora Society.St.Paul,MN, 1989:35-38. [18]Kaufmann M J, Gerdemann J W. Root and stem rot of soybean caused by Phytophthora sojae[J]. Phytopathology, 1958, 48: 201-208. [19]Yang X B, Ruff R L, Meng X Q, et al. Race of Phytophthora sojaein Iowa soybean fields[J]. Plant Disease, 1996, 80: 1418-1420. [20]许修宏, 吕慧颖, 曲娟娟, 等. 大豆疫霉根腐病菌生理小种鉴定及毒性分析[J]. 植物保护学报, 2003, 30(2): 125-128.(Xu X H, Lu H Y, Qu J J, et al. Phytophthora sojae races in Northeast of China and the virulence evaluation of the isolates[J]. Journal of Plant Protection, 2003,30(2):125-128.) [21]张淑珍, 吴俊江, 徐鹏飞, 等. 黑龙江省大豆疫霉根腐病菌毒力类型及15号小种的首次报道[J]. 中国油料作物学报, 2008, 30(2): 229-234. (Zhang S Z, Wu J J, Xu P F, et al. Identification of virulence Phytophthora sojaein Heilongjiang province and the first report on race 15 in China[J]. Chinese Journal of Oil Crop Sciences, 2008, 30(2): 229-234.) [22]马淑梅. 大豆疫霉根腐病3号生理小种抗性遗传及抗病种质创新[J]. 黑龙江大学自然科学学报, 2008, 25(3): 347-350. (Ma S M. Study on the resistance to No.3 biological race of phytohthora root rot of soybean germplasms and breeding for resistant varieties[J]. Journal of Natural Science of Heilongjiang University, 2008, 25(3): 347-350.) [23]李长松, 路兴波, 尚佑芬, 等. 大豆疫霉根腐病菌的分离及品种抗病性鉴定[J].吉林农业大学学报, 1998, 20: 133. (Li C S, Lu X B, Shang Y F, et al. Isolation of Phytophthora megasperma yf.sp.giycinea and screeing of resistant soybean cultivars[J].Journal of Jilin Agricultural University, 1998, 20: 133.) [24]朱振东, 霍云龙, 王晓鸣, 等. 大豆疫霉根腐病抗源筛选[J]. 植物遗传资源学报, 2006, 7(1): 24-30. (Zhu Z D, Huo Y L, Wang X M, et al. Screening for resistance sources to Phytophthora root rot in soybean[J]. Journal of Plant Genetic Resources, 2006, 7(1): 24-30.) [25]李宝英. 大豆种质资源对疫霉根腐病的抗性鉴定[J]. 农机化研究, 2005(5): 205-206. (Li B Y. Identification the resistance of soybean germplasm to Phytophthora root rot[J]. Journal of Agricultural Mechanization Research, 2005(5): 205-206.) [26]Lohnes D G, Nickell C D, Schmitthenner A F. Origin of soybean alleles for Phytophthora resistance in China[J]. Crop Science, 1996, 36: 1689-1692. [27]Kyle D E, Nickell C D, Nelson R L, et al. Response of soybean accessions from provinces in Southern China to Phytophthora soja[J]. Plant Disease, 1998, 82: 555-559. [28]李福山.中国野生大豆资源的地理分布及生态分化研究[J].中国农业科学, 1993, 26(2) : 47-55. (Li F S. Studies on the ecological and geographical distribution of the chinese resources of wild soybean(G.Sojae)[J]. Scientia Agricultura Sinica, 1993, 26(2): 47-55.)
相似文献/References:
[1]韩英鹏,程 章,赵 雪,等.大豆花叶病毒病和疫霉根腐病抗性的SSR标记辅助鉴定[J].大豆科学,2013,32(06):740.[doi:10.11861/j.issn.1000-9841.2013.06.0740]
[2]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶（POD）活性变化[J].大豆科学,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
CHENG Peng,XU Peng-fei,FAN Su-jie,et al.Response of POD Activity in Glycine soja ?Inoculated by Phytophthora sojae[J].Soybean Science,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
[3]韩英鹏,赵 雪,李修平,等.大豆种质对花叶病毒病和疫霉根腐病抗病性的SSR标记辅助鉴定[J].大豆科学,2014,33(01):27.[doi:10.11861/j.issn.1000-9841.2014.01.0027]
HAN Yingpeng,ZHAO Xue,LI Xiuping,et al.SSR Identification of Soybean Cultivar with Resistance to Soybean Mosaic Virus and Phytophthpra Root Rot[J].Soybean Science,2014,33(02):27.[doi:10.11861/j.issn.1000-9841.2014.01.0027]
[4]申宏波,姚文秋,于永梅,等.不同类型生物农药对大豆疫霉根腐病的防治效果[J].大豆科学,2011,30(06):1054.[doi:10.11861/j.issn.1000-9841.2011.06.1054]
SHEN Hong-bo,YAO Wen-qiu,YU Yong-mei,et al.Control Efficiency of Different Biopesticides on Soybean Phytophthora Root Rot[J].Soybean Science,2011,30(02):1054.[doi:10.11861/j.issn.1000-9841.2011.06.1054]
[5]程莹鑫,梁吉利,刘庆莉,等.疫霉根腐病菌毒素对大豆不同组织中总多酚含量的影响[J].大豆科学,2014,33(04):524.[doi:10.11861/j.issn.1000-9841.2014.04.0524]
CHENG Ying-xin,LIANG Ji-li,LIU Qing-li,et al.Changes of Total Polyphenol Content in Soybean Treated by Pathotoxin Produced by Phytophthora sojae[J].Soybean Science,2014,33(02):524.[doi:10.11861/j.issn.1000-9841.2014.04.0524]
[6]张淑珍,靳立梅,徐鹏飞,等.野生大豆接种大豆疫霉根腐病后苯丙氨酸解氨酶（PAL）活性的变化[J].大豆科学,2009,28(06):1044.[doi:10.11861/j.issn.1000-9841.2009.06.1044]
ZHANG Shu-zhen,JIN Li-mei,XU Peng-fei,et al.Response of PAL Activity to Phytophthora sojae Inoculation in Glycine soja[J].Soybean Science,2009,28(02):1044.[doi:10.11861/j.issn.1000-9841.2009.06.1044]
[7]任龙翚,张宝强,武晓玲,等.大豆种质对大豆疫霉菌株Pm8的抗性分析[J].大豆科学,2010,29(01):77.[doi:10.11861/j.issn.1000-9841.2010.01.0077]
REN Long-hui,ZHANG Bao-qiang,WU Xiao-ing,et al.Resistance of Soybean Germplasm to Phytophthora sojae Pm8[J].Soybean Science,2010,29(02):77.[doi:10.11861/j.issn.1000-9841.2010.01.0077]
[8]马淑梅,金娜,邵红涛.黑龙江省大豆疫霉根腐病菌毒力类型分布及品种抗性评价[J].大豆科学,2010,29(03):466.[doi:10.11861/j.issn.1000-9841.2010.03.0466]
MA Shu-mei,JIN Na,SHAO Hong-tao.Virulence Distribution and Cultivars Resistance Evaluation of Phytophthora Megasperma in Heilongjiang Province[J].Soybean Science,2010,29(02):466.[doi:10.11861/j.issn.1000-9841.2010.03.0466]
[9]任海龙,宋恩亮,马启彬,等.南方三省（区）抗大豆疫霉根腐病野生大豆资源的筛选[J].大豆科学,2010,29(06):1012.[doi:10.11861/j.issn.1000-9841.2010.06.1012]
REN Hai-long,SONG En-liang,MA Qi-bin,et al.Screening for Resistance Sources to Phytophthora Root Rot in Glycine soja from Three Provinces of Southern China[J].Soybean Science,2010,29(02):1012.[doi:10.11861/j.issn.1000-9841.2010.06.1012]
[10]于安亮,徐鹏飞,陈晨,等.大豆疫霉根腐病子叶接种法抗病性鉴定[J].大豆科学,2009,28(05):879.[doi:10.11861/j.issn.1000-9841.2009.05.0879]
YU An-liang,XU Peng-fei,CHEN Chen,et al.Screening on Soybean Resistance to Phytophthora sojae using Cotyledon Inoculation Method[J].Soybean Science,2009,28(02):879.[doi:10.11861/j.issn.1000-9841.2009.05.0879]
[11]张武,李春杰,焦晓丹,等.黑龙江省北部地区大豆种质对疫霉根腐病抗性评价[J].大豆科学,2024,43(04):496.[doi:10.11861/j.issn.10009841.2024.04.0496]
备注/Memo
基金项目：国家自然科学基金资助项目(30671317, 30810103063, 30400285)；黑龙江省新世纪人才培养计划资助项目 (NCET-06-007)；黑龙江省自然科学基金资助项目（C200814）；黑龙江省农业科学院博士后基金资助项目（LRB06-010）；中国博士后基金资助项目（20060400835）；博士后落户黑龙江科研启动基金资助项目(LBH-Q05032)。