[1]韩英鹏,程 章,赵 雪,等.大豆花叶病毒病和疫霉根腐病抗性的SSR标记辅助鉴定[J].大豆科学,2013,32(06):740-743.[doi:10.11861/j.issn.1000-9841.2013.06.0740]
点击复制

大豆花叶病毒病和疫霉根腐病抗性的SSR标记辅助鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年06期 页码: 740-743 栏目: 出版日期: 2013-12-25
Title:
SSR Identification of Soybean Line with Resistance to Both Soybean Mosaic? Virus and Phytophthpra Root Rot
作者:
?韩英鹏; 程 章; 赵 雪; 滕卫丽; 李文滨
?（东北农业大学大豆生物学教育部重点实验室，东北农业大学农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030）
Author(s):
?（Key Laboratory of Soybean Biology in Chinese Ministry of Education，Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,China）
关键词:
大豆花叶病毒病; 大豆疫霉根腐病; 抗病性; SSR标记鉴定
Keywords:
Soybean mosaic virus; Phytophthora root rot; Resistance; SSR identification
DOI:
10.11861/j.issn.1000-9841.2013.06.0740
文献标志码:
A
摘要:
?采用抗花叶病毒病品种东农93046、抗疫霉根腐病品种Conrad、耐疫霉根腐病品种合丰25和高产品种东农L5两两杂交后再复交衍生的146个F2:5重组自交系，利用与大豆花叶病毒病抗性基因相关的SSR标记Satt114，与大豆疫霉根腐病抗性相关的分子标记Satt325、Satt343、Satt428、Satt005、Satt600、Satt611、Satt689、Satt579、Satt252、Satt274进行分子辅助鉴定，进而分别通过摩擦接种法和菌土法来验证分子辅助鉴定的准确性。结果表明：通过Satt114分子辅助鉴定共发现32个抗大豆花叶病毒病家系，经摩擦接种法表型验证其中28个家系表现为抗病，即分子辅助鉴定的准确性为87.5%。通过与大豆疫霉根腐病相关的10个SSR标记的分子辅助鉴定，共发现23个家系含有4～8个抗性位点，经菌土法表型验证其中含有4，5，6，7，8个抗病位点家系的病害损失率分别可达61.11%、47.08%、32.92%、26.11%、18.34%，即后代家系聚合越多的QTL则抗大豆疫霉根腐病能力越强。另外，本研究得到了4份既高抗大豆花叶病毒病又高抗大豆疫霉根腐病的新种质。
Abstract:
?Soybean mosaic virus(SMV)and phytophthora root rot(PRR)are both worldwide soybean diseases,which seriously affect the yield and quality of soybean.Selecting resistance varieties is one of the most effective measures to control these two diseases.Traditional program for varieties with resistance to disease is timeconsuming,laborious and inefficient.SSR marker and molecularassisted breeding make effective selection of varieties with resistance to disease possible.A recombinant intercross line population including 146 F2∶〖KG-*2〗5 line was derived from a cross between F1(Dongnong93046×Hefeng25)× F1(Congrad×DongnongL5).SSR marker Satt114,identified by Teng et al(2005),and Satt325,Satt343,Satt428,Satt005,Satt600,Satt611,Satt689,Satt579,Satt252,Satt274 identified by Li et al(2010)were used to analyze these RI line with resistance to SMV and PRR.Moreover,phenotypic analysis for SMV and PRR was used to evaluate SSR identification.A total of 32 lines are resistance to SMV,28 of which are verified through friction inoculation test.The accuracy of molecular assistedbreeding is 87.5%.A total of 23 lines have 48 resistance loci to PRR,loss rate of which are 61.11%,47.08%,32.92%,26.11%,18.34%,respectively.In this study,four lines are highly resistant to both SMV and PRR.

相似文献/References:

[1]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶（POD）活性变化[J].大豆科学,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
　CHENG Peng,XU Peng-fei,FAN Su-jie,et al.Response of POD Activity in Glycine soja ?Inoculated by Phytophthora sojae[J].Soybean Science,2013,32(06):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
[2]史凤玉,朱英波,龙茹,等.大豆花叶病毒诱导的野生大豆抑制差减文库构建及初步分析[J].大豆科学,2011,30(06):968.[doi:10.11861/j.issn.1000-9841.2011.06.0968]
　SHI Feng-yu,ZHU Ying-bo,LONG Ru,et al.Construction and Primary Analysis of a SSH Library of Wild Soybean Induced by SMV[J].Soybean Science,2011,30(06):968.[doi:10.11861/j.issn.1000-9841.2011.06.0968]
[3]申宏波,姚文秋,于永梅,等.不同类型生物农药对大豆疫霉根腐病的防治效果[J].大豆科学,2011,30(06):1054.[doi:10.11861/j.issn.1000-9841.2011.06.1054]
　SHEN Hong-bo,YAO Wen-qiu,YU Yong-mei,et al.Control Efficiency of Different Biopesticides on Soybean Phytophthora Root Rot[J].Soybean Science,2011,30(06):1054.[doi:10.11861/j.issn.1000-9841.2011.06.1054]
[4]程莹鑫,梁吉利,刘庆莉,等.疫霉根腐病菌毒素对大豆不同组织中总多酚含量的影响[J].大豆科学,2014,33(04):524.[doi:10.11861/j.issn.1000-9841.2014.04.0524]
　CHENG Ying-xin,LIANG Ji-li,LIU Qing-li,et al.Changes of Total Polyphenol Content in Soybean Treated by Pathotoxin Produced by Phytophthora sojae[J].Soybean Science,2014,33(06):524.[doi:10.11861/j.issn.1000-9841.2014.04.0524]
[5]张淑珍,靳立梅,徐鹏飞,等.野生大豆接种大豆疫霉根腐病后苯丙氨酸解氨酶（PAL）活性的变化[J].大豆科学,2009,28(06):1044.[doi:10.11861/j.issn.1000-9841.2009.06.1044]
　ZHANG Shu-zhen,JIN Li-mei,XU Peng-fei,et al.Response of PAL Activity to Phytophthora sojae Inoculation in Glycine soja[J].Soybean Science,2009,28(06):1044.[doi:10.11861/j.issn.1000-9841.2009.06.1044]
[6]任龙翚,张宝强,武晓玲,等.大豆种质对大豆疫霉菌株Pm8的抗性分析[J].大豆科学,2010,29(01):77.[doi:10.11861/j.issn.1000-9841.2010.01.0077]
　REN Long-hui,ZHANG Bao-qiang,WU Xiao-ing,et al.Resistance of Soybean Germplasm to Phytophthora sojae Pm8[J].Soybean Science,2010,29(06):77.[doi:10.11861/j.issn.1000-9841.2010.01.0077]
[7]徐鹏飞,吴俊江,范素杰,等.大豆疫霉根腐病菌的分离鉴定及种质资源对3号生理小种的抗性评价[J].大豆科学,2010,29(02):272.[doi:10.11861/j.issn.1000-9841.2010.02.0272]
　XU Peng-fei,WU Jun-jiang,FAN Su-jie,et al.Isolation of Phytophthora sojae and Identification of Resistance to Race 3 in Soybean Germplasm[J].Soybean Science,2010,29(06):272.[doi:10.11861/j.issn.1000-9841.2010.02.0272]
[8]马淑梅,金娜,邵红涛.黑龙江省大豆疫霉根腐病菌毒力类型分布及品种抗性评价[J].大豆科学,2010,29(03):466.[doi:10.11861/j.issn.1000-9841.2010.03.0466]
　MA Shu-mei,JIN Na,SHAO Hong-tao.Virulence Distribution and Cultivars Resistance Evaluation of Phytophthora Megasperma in Heilongjiang Province[J].Soybean Science,2010,29(06):466.[doi:10.11861/j.issn.1000-9841.2010.03.0466]
[9]史凤玉,朱英波,龙茹,等.野生大豆抗大豆花叶病毒病评价、聚类及性状间相关分析[J].大豆科学,2010,29(06):976.[doi:10.11861/j.issn.1000-9841.2010.06.0976]
　SHI Feng-yu,ZHU Ying-bo,LONG Ru,et al.Evaluation, Cluster Analysis for Glycine soja Resistant to Soybean Mosaic Virus (SMV) and Correlation Analysis between Characters[J].Soybean Science,2010,29(06):976.[doi:10.11861/j.issn.1000-9841.2010.06.0976]
[10]任海龙,宋恩亮,马启彬,等.南方三省（区）抗大豆疫霉根腐病野生大豆资源的筛选[J].大豆科学,2010,29(06):1012.[doi:10.11861/j.issn.1000-9841.2010.06.1012]
　REN Hai-long,SONG En-liang,MA Qi-bin,et al.Screening for Resistance Sources to Phytophthora Root Rot in Glycine soja from Three Provinces of Southern China[J].Soybean Science,2010,29(06):1012.[doi:10.11861/j.issn.1000-9841.2010.06.1012]
[11]韩英鹏,赵 雪,李修平,等.大豆种质对花叶病毒病和疫霉根腐病抗病性的SSR标记辅助鉴定[J].大豆科学,2014,33(01):27.[doi:10.11861/j.issn.1000-9841.2014.01.0027]
　HAN Yingpeng,ZHAO Xue,LI Xiuping,et al.SSR Identification of Soybean Cultivar with Resistance to Soybean Mosaic Virus and Phytophthpra Root Rot[J].Soybean Science,2014,33(06):27.[doi:10.11861/j.issn.1000-9841.2014.01.0027]

备注/Memo

?国家重点基础研究发展计划“973计划”前期项目（2012CB126311）；国家自然科学基金（31201227）；国家“十二五”科技支撑计划（2011BAD35B06-1）；现代农业产业技术体系（CARS-04-PS04）； 中国博士后项目（20110491024）；黑龙江省博士后项目(LBH11220，LBH-TZ1210)；黑龙江省教育厅骨干教师资助项目（1252G014）；黑龙江省教育厅新世纪项目优秀人才的资助项目（1253-NCET-005）；教育部博士点项目（20122325120012）；东北农业大学博士后启动金项目（2012RCB11）。

更新日期/Last Update: 2014-02-28