[1]张俐俐,谷维,雷勃钧,等.应用化学诱变法筛选抗草甘膦大豆突变株系[J].大豆科学,2009,28(05):938-940.[doi:10.11861/j.issn.1000-9841.2009.05.0938]
　ZHANG Li-li,GU Wei,LEI Bo-jun,et al.Glyphosate Resistant Mutant Strain of Soybean Filtered by Chemomorphosis[J].Soybean Science,2009,28(05):938-940.[doi:10.11861/j.issn.1000-9841.2009.05.0938]
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应用化学诱变法筛选抗草甘膦大豆突变株系

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年05期 页码: 938-940 栏目: 出版日期: 2009-10-25
Title:
Glyphosate Resistant Mutant Strain of Soybean Filtered by Chemomorphosis
文章编号:
1000-9841(2009)05-0938-03
作者:
张俐俐1; 谷维2; 雷勃钧1; 吕晓波1; 李铁1
1.黑龙江省农业科学院 生物技术研究所，黑龙江 哈尔滨 150086；
2.黑龙江省农业科学院 作物营养实用技术研究所，黑龙江 哈尔滨 150086
Author(s):
ZHANG Li-li1; GU Wei2; LEI Bo-jun1; LU Xiao-bo1; LI Tie1
1.Biotechnology Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086；
?2.Crop Nutrition Practical Technology Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
关键词:
大豆; 甲基磺酸乙酯; 叠氮化钠; 化学诱变; 草甘膦抗性
Keywords:
Soybean; Ethyl methane sulphonate; Sodium azide; Chemomorphosis; Glyphosate resistance
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.05.0938
文献标志码:
A
摘要:
利用化学诱变剂甲基磺酸乙酯（EMS）和叠氮化钠，对黑龙江省大面积推广的6个农艺性状优良的大豆品种进行种子处理。不同品种按不同处理剂量分区种植，按常规法单株选择收获。不同浓度诱变剂处理大豆种子的M1代成活率均高于半致死浓度处理；对M3代35518个植株于3叶期进行喷洒1.31ai.kg.hm2草甘膦浓度鉴定，其中40株生长正常，所获抗性植株是经5 mmol.L-1叠氮化钠诱变处理的绥农10和黑农44。2008年继续对M4代进行鉴定，抗草甘膦特性可以稳定遗传。应用化学诱变法可以在后代群体中筛选到具有抗草甘膦特性的突变株系。
Abstract:
To screen soybean lines resistant to glyphosate through chemomorphosis，six soybean varieties widly planted in Heilongjiang Province were dealt with chemical mutagen ethyl methane sulphonate（EMS）and sodium azide by seed soaking.The survival rate of soybean seeds in M1generation was higher than that of semi-lethal concentrations.In M3generation，35518 plants were identified by spraying 1.31ai.kg.ha-1glyphosate at three-leaf stage，from which a total of 40 resistant plants showed normal growth，and they were Suinong 10 and Heinong 44 dealt with 5 mmol.L-1 sodium azide.The M4generation of the 40 survival lines also showed glyphosate resistance.Results suggest chemical mutagen could be used in selecting soybean mutant lines resistant to glyphosate.

参考文献/References:

[1]苏少泉.抗除草剂作物的过去、现在与未来[J].世界农药，2006，28（2）：1-4.(Su S Q.The past，present and future of atrizine residues plant[J].World Pesticide，2006，28(2):1-4.)

[2]贾士荣.转基因植物的环境及食品安全性[J].生物工程进展，1997，17(6):37-42.(Ja S R.The safety evaluation of transgenic plants[J].Biology Engineering Evolve，1997，17(6):37-42.)
[3]苏少泉.转基因抗除草剂作物与除草剂开发及应用[J].农药，2002，41(7):3-7.(Su S Q.Exploitation and application of transgenetic of plants resistant to Herbicide and Herbicide[J].Pesticide，2002，41(7):3-7.)
[4]James C.Gobal status and distribution of commericial transgenic crop in 1997[J].Biotech Develop Monitor，1998，35:9-12.
[5]杨崇良，路兴波，张君亭.世界农业转基因生物、产品研发及其安全性监管Ⅲ.农业转基因生物及其产品安全评价与管理[J].山东农业科学，2005，3：67-70.(Yang C L，Lu X B，Zhang J T.Management of investigation and invent and safty of transgenetic biology production of world agriculture Ⅲ Safety appraisal and management of agricultural transgenetic biology and its production[J].Shandong Agricultural Sciences,2005，3：67-70.)
[6]李海军，孟庆民，祝丽英，等.抗除草剂作物的选育研究概况[J].杂粮作物，2003，23(1):30-32.(Li H J，Meng Q M，Zhu L Y.Development of breeding Herbicide-resistant crops[J].Rain Fed Crops，2003，23(1):30-32.)
[7]马惠平，赵永亮，杨光宇.诱变技术在农作物育种中的应用[J].遗传，1998，20(4):41-43.(Ma H P，Zhao Y L，Yang G Y.Application of induced technology on the breeding of plant[J].Heredity，1998，20（4)：41-43.)

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备注/Memo

基金项目：黑龙江省自然基金（Ｂ类）资助项目（ＺＪＮ０４-０４０２）。

作者简介：张俐俐（1980-），女，硕士，研究实习员，现主要从事大豆诱变育种及生物技术研究。E-mail:zll20041001@163.com。
通讯作者：吕晓波。E-mail:xiaobo6032@163.com

更新日期/Last Update: 2014-09-19