[1]陈立杰,刘晓杰,段玉玺,等.轮作和连作田大豆胞囊线虫胞囊上真菌定殖动态[J].大豆科学,2009,28(02):266-270.[doi:10.11861/j.issn.1000-9841.2009.02.0266]
　CHEN Li-jie,LIU Xiao-jie,DUAN Yu-xi,et al.Dynamics of Fungal Colonization on Cyst in Rotation and Continuous Cropping of Soybean[J].Soybean Science,2009,28(02):266-270.[doi:10.11861/j.issn.1000-9841.2009.02.0266]
点击复制

轮作和连作田大豆胞囊线虫胞囊上真菌定殖动态

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年02期 页码: 266-270 栏目: 出版日期: 2009-04-25
Title:
Dynamics of Fungal Colonization on Cyst in Rotation and Continuous Cropping of Soybean
文章编号:
1000-9841(2009)02-0266-05
作者:
陈立杰; 刘晓杰; 段玉玺; 王媛媛; 朱晓峰
沈阳农业大学植物保护学院，辽宁 沈阳 110161
Author(s):
CHEN Li-jie; LIU Xiao-jie; DUAN Yu-xi; WANG Yuan-yuan; ZHU Xiao-feng
College of Plant Protection，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
关键词:
大豆; 胞囊; 真菌; 定殖动态
Keywords:
Soybean; Cyst; Fungi; Dynamic of Colonization
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0266
文献标志码:
A
摘要:
研究沈阳市汪家乡连作6年和轮作大豆田中胞囊数量以及大豆不同生长期胞囊上定殖真菌的种群及数量变化。结果表明：在整个大豆生长期中，不饱满胞囊量高于饱满胞囊量，连作田中胞囊总量、不饱满胞囊量高于轮作田。从胞囊上分离到的真菌以镰孢菌居多，在大豆不同生长时期镰孢菌属的分离频率不同，其中尖孢镰孢菌Fusarium.oxysporum为优势种群，且连作田中数量多于轮作田；除镰孢菌属真菌外，轮作田中胞囊上分离到的青霉属和粘帚霉属真菌数量最多，还分离到毛霉属、毛壳菌属和拟青霉属；而连作田中胞囊上分离到的真菌中粘帚霉属数量最多，其他各属真菌的数量都很少，其中轮枝菌属和腐质霉属在轮作田胞囊上未分离到。镰孢菌的定殖数量与胞囊的饱满程度有关，而青霉、粘帚霉和拟青霉等其他真菌种群也影响到胞囊的饱满程度。
Abstract:
The number of cyst and the fungal colonizing on cyst in the fields of rotation and continuous cropping of soybean for six years were studied at Village Wangjia in Shenyang city.The results showed that unplump cysts were more than plump ones in both rotation and continuous fields.While the total numbers of cysts and numbers of unplump cysts isolated from continuous cropping were more than those in rotation field.Among the fungi isolated from cysts Fusarium presented the highest isolations.The frequency of isolated Fusarium was different in 5 soybean growing stages.F.oxysporum was a dominant population and its isolations were more in continuous cropping than those in rotation field.In rotation field，Penicillium and Gliocladium were another two dominant populations.The other isolated fungi were Mucor，Chaetomium and Peacilomyce.In continuous cropping field，the numbers of Gliocladium were higher but others were lower.Verticillium and Humicola were only isolated from continuous cropping field but not found in rotation field.Our results showed a trend that the numbers ofFusarium colonized on cysts were correlated with the numbers of plump cysts among tested samples.The other fungi such as Penicillium，Gliocladium and Peacilomyces affected the plump degree of cysts.

参考文献/References:

[1]陈宗泽，殷勤燕.大豆连作对土壤微生物生物量的影响[J].大豆通报，1997，(6)：15(Chen Z Z，Yin Q Y.The effect of biomass with soil microorganisms under soybean continuous cropping[J].Soybean Bullelin，1997(6)：15.)

[2]王金龙，徐冉，陈存来，等.大豆连作下土壤环境条件变化的概述[J].大豆科学，2000，19(4):367-371.(Wang J L，Xu R，Chen C L，et al.General review in the study of barrier mechanism caused by continuous soybean cropping[J].Soybean Science，2000，19(4):367-371.)
[3]辛洪生，胡瑞轩，张风彬.黑龙江省大豆连作施肥技术[J].种子世界，2002(1):41-43.(Xin H S，Hu R X，Zhang F B.Technology of fertilization with soybean in Heilongjiang province[J].Seed Word，2002(1)：41-43.)
[4]郑良永，胡剑非，林昌华，等.作物连作障碍的产生及防治[J].热带农业科学，2005，25(2):58-62.(Zheng L Y ，Hu J F，Lin C H，et al.The production of succession cropping obstacles and its prevention and cure steps[J].Chinese Journal of Tropical Agriculture，2005，25(2):58-62.)
[5]孙漫红，刘杏忠.连作土壤中大豆胞囊线虫种群数量减少的原因探讨[J]，植物病理学报，30(4):353-363.(Sun M H，Liu X Z.Suppressive soils of soybean cyst nematode in China[J].Acta Phytopathologica Sinica，2000，30(4):353-363.)
[6]陈立杰，段玉玺，范圣长，等.大豆胞囊线虫病的生防因子研究进展[J].西北农林科技大学学报(自然科学版)，2005，33(增):190-194.(Chen L J，Duan Y X，Fan S C，et al.Advances in antagonists of soybean cyst nematode[J].Journal of Northwest Sci-Tech University of Agriculture and Forest，2005，33(Supply):190-194.)
[7]刘维志.植物线虫学研究技术[M].沈阳:辽宁科学技术出版社，1995:47-49.(Liu W Z.Technology for nematological research[M].Shenyang:Liaoning Science Technology Press，1995:47-49.)
[8]陆家云.植物病原真菌学[M].北京:中国农业出版社，2000.(Lu J Y.Plant pathogenic fungi[M].Beijing：Chinese Agricultural Press，2000.)
[9]陈鸿逵，王拱辰.浙江镰刀菌志[M].杭州:浙江科技出版社，1992.(Chen H K，Wang G C.Zhejiang Fusarium[M].Hangzhou：Zhejiang Science and Technology Press，1992.)
[10]陈宗泽，殷勤燕，戴秉丽，等.连作大豆土壤病原菌的分离及其致病性的研究[J].吉林农业科学，1999，24(2):36-39.(Chen Z Z，Yin Q Y，Dai B L，et al.Study on isolation and pathogenicity of soil pathogen in continuous cropping systems of soybean[J].Jinlin Agricultural Sciences，1999，24(2):36-39.)
[11]马汇泉.大豆根腐病病原菌种类及其生物学研究[J].黑龙江八一农垦大学学报，1988(2)：115-121.(Ma H Q.Studies on the ecology of root rot of soybean[J].Journal of Heilongjiang August First Land Reclamation University，1988，(2)：115-121.)
[12]刘杏忠，张东升，武修英，等.定殖于大豆胞囊线虫胞囊内真菌的初步研究[J] .北京农业大学学报，1991，17(3)：87-91.(Liu X Z，Zhang D S，Wu X Y，et al.Fungal colonization in cyst on soybean cyst nematode[J].Journal of Beijing Agriculture University，1991：17(3):87-91.)
[13]Chen Z X，Chen S Y,Dickson D W.Nematology-advances and perspectives，Vol(2)Nematode management and utilization[M].Beijing:Tsinghua University Press，CABI Publishing,2004:979-1071.
[14] Gao X，Jackson T A，Hartman G L，et al.Interactions between the soybean cyst nematode and Fusarium solani f.sp.glycines based on greenhouse factorial experiments[J].Phytopathology，2006,96：(12):1409-1415.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：霍英东青年教师基金资助项目(101033)；辽宁省优秀人才支持计划资助项目(RC-05-18)；科技部成果转化基金资助项目（05EFN212100059）。

作者简介：陈立杰 (1971-),女,博士,教授,主要从事植物病理学和线虫学研究。E-mail:chenlj@syau.edu.cn。

更新日期/Last Update: 2014-10-03