[1]吕晓波,王宏燕,刘琦,等.抗草甘膦转基因大豆（RRS）在黑土生态系统种植的安全性研究[J].大豆科学,2009,28(02):260-265.[doi:10.11861/j.issn.1000-9841.2009.02.0260]
　L Xiao-bo,WANG Hong-yan,LIU Qi,et al.Biosafety of Roundup Ready Soybean（RRS）Planted in Black Soil Ecosystem[J].Soybean Science,2009,28(02):260-265.[doi:10.11861/j.issn.1000-9841.2009.02.0260]
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抗草甘膦转基因大豆（RRS）在黑土生态系统种植的安全性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年02期 页码: 260-265 栏目: 出版日期: 2009-04-25
Title:
Biosafety of Roundup Ready Soybean（RRS）Planted in Black Soil Ecosystem
文章编号:
1000-9841(2009)02-0260-06
作者:
吕晓波1; 王宏燕2; 刘琦1; 3; 赵光2; 李希臣1; 3; 徐广惠2; 张俐俐1; 3; 刘佳2; 刘昭军1; 李宁2; 李铁1; 雷勃钧1
1 黑龙江省农业科学院生物技术研究所，黑龙江 哈尔滨 150086；
2东北农业大学资源环境学院，黑龙江 哈尔滨 150030；
3黑龙江省作物与家畜分子育种重点实验室，黑龙江 哈尔滨 150086
Author(s):
L Xiao-bo1; WANG Hong-yan2; LIU Qi1; 3; ZHAO Guang1; LI Xi-chen1; 3; XU Guang-hui2; ZHANG Li-li1; 3; LIU Jia2; LIU Zhao-jun1; LI Ning2; LI Tie1; LEI Bo-jun1
1Institute of Biotechnology，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang；
2College of Resource and Environment，Northeast Agricultural University，Harbin 150030，Heilongjiang；
3Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang，Harbin 150086，Heilongjiang，China
关键词:
抗草甘膦基因; 转基因大豆; 黑土生态系统; 土壤细菌多样性
Keywords:
CP4－EPSPS gene;  Transgenic soybean;  Black soil ecosystem;  Diversities of rhziosphere bacteria
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0260
文献标志码:
A
摘要:
为明确抗草甘膦转基因大豆（RRS）在黑土生态系统种植的安全性，经过连续3年田间和盆栽试验，分析了抗草甘膦转基因漂移（漂流）可能性，及抗草甘膦转基因大豆（RRS）在黑土区对根际生态系统微生物数量，氮代谢和两种土壤酶活性的影响。结果表明：在自然条件下花粉漂移几乎是不可能的，但如人为加大虫媒传播（大于10头m-2），抗草甘膦基因的漂移概率接近0.05%，漂移距离为0.7 m。RRS除对根际真菌数影响不显著外，对根际土壤细菌、放线菌、氨化和硝化细菌均表现降低趋势。在大豆不同生育期，RRS除对根际土壤真菌数影响不显著外，对根际土壤细菌，放线菌，氨化和硝化细菌均表现降低趋势；RRS根际土壤细菌多样性指数与均匀度指数均低于亲本RRS-S；氨化强度与RRS-S相比差异不显著，RRS根际土壤硝化强度显著低于亲本RRS-S；RRS降低了过氧化氢酶和脲酶活性。
Abstract:
In the three-year field and pot experiments，this study investigates possibility of the CP4－EPSPS gene flow of Roundup Ready Soybean（RRS）in black soil ecosystem，and impacts of RRS on numbers of its rhizosphere microorganisms，N-cycling and some enzymes in soil.According to the field experiments，the CP4－EPSPS gene flow did not happen in natural condition，but under insects(more than 10 insects per square meter) helping to pollen，the floating possibility of this gene was 0.05%，and the floating distance was 0.7 meter.In different soybean growth stages，in rhizosphere soil of RRS，the amount of bacteria，actinomycete，ammonifying bacteria，and nitrifying bacteria showed decreasing tendencies，except no effect on total number of fungus.The bacterial diversity indexes and homogeneous degrees indexes in rhizospheric soil of RRS were lower than that of its parent RRS-S.The difference of ammonification intensity was not significant in RRS and RRS-S rhizosphere soil，and nitrification intensity in RRS was significantly lower than that in RRS-S rhizosphere soil in different growth stages and RRS inhibited activities of catalase and urease.

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备注/Memo

基金项目：黑龙江省自然科学基金（Ｂ类）资助项目（ＺＪＮ０４-０４０２）。

作者简介：吕晓波（1964-），女，博士，主要从事大豆、小麦生物技术研究。E-mail: xb6032@163.com。

更新日期/Last Update: 2014-10-03