[1]杨喆,刘丽君,高明杰,等.大豆高蛋白基因分子标记及其在大豆育种中的应用[J].大豆科学,2008,27(02):186-189.[doi:10.11861/j.issn.1000-9841.2008.02.0186]
　YANG Zhe,LIU Li-jun,GAO Ming-jie,et al.QTL Tagging for High Protein Gene and Using Molecular Marker Assistant Selection in Soybean Breeding[J].Soybean Science,2008,27(02):186-189.[doi:10.11861/j.issn.1000-9841.2008.02.0186]
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大豆高蛋白基因分子标记及其在大豆育种中的应用

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 186-189 栏目: 出版日期: 2008-04-30
Title:
QTL Tagging for High Protein Gene and Using Molecular Marker Assistant Selection in Soybean Breeding
文章编号:
1000-9841(2008)02-0186-04
作者:
杨喆; 刘丽君; 高明杰; 蒲国锋; 张雷
黑龙江省农业科学院大豆研究所，黑龙江 哈尔滨 150086
Author(s):
YANG Zhe; LIU Li-jun; GAO Ming-jie; PU Guo-feng; ZHANG Lei
Soybean Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
关键词:
大豆; 高蛋白; 分子标记辅助育种; QTL;  SSR
Keywords:
Soybean; High protein; Molecular marker assistant breeding; QTL; SSR
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0186
文献标志码:
A
摘要:
选用高蛋白大豆黑农35和高油大豆黑农45作为亲本杂交获得F2分离群体，进行大豆高蛋白基因SSR分子标记。共筛选覆盖大豆全基因组的251对SSR引物，其中40对SSR引物在亲本间具有多态性，用这40对SSR引物分别对F2分离群体进行扩增，用Mapmaker Exp3.0和Mapmaker QTL1.1软件进行作图和定位分析。定位得到高蛋白QTL 1个，与satt532连锁，遗传距离为0.2 cM，贡献率为32.7％，位于大豆公共遗传图谱的D1a+Q连锁群。利用该引物在不同大豆材料中进行高蛋白材料检测和筛选，在56份高蛋白材料中筛选到47份，检出率达到83.93％。说明引物satt532具有一定的检测通用性，可以利用它筛选高蛋白大豆材料。
Abstract:
Soybean protein content is a quantitative trait controlled by multi-genes，and tagging these genes by using molecular markers has strong applied values.F2 population made from Heinong 35×Heinong 45 were used to map high protein genes.251 pair of SSR primers covering the whole soybean genome were screened，and 40 pair of them were polymorphic between the parents.The genetic linkage map was constructed with Mapmaker Exp3.0 and QTL analysis was done by using Mapmaker QTL1.1.One high protein QTL linking with satt532 in the LG D1a+Q of soybean consensus map was tagged，the genetic distance was 0.2 cM，the variance ratio was 32.7%.The primer satt532 was used to exam and screen the high protein soybean materials，47 shares were screened from 56 ones，and the ratio of the examination reached 83.93%.The result suggests that the primer of satt532 has the common identity and can be used to screen the high protein soybeans.

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备注/Memo

基金项目：黑龙江省农业科学院科研计划资助项目。

作者简介：杨喆（1978-），男，助理研究员，硕士，现从事大豆分子标记及大豆遗传育种研究。E-mail：yz78100@163.com。

更新日期/Last Update: 2014-10-10