[1]吴明生,田雷,康俊梅,等.大豆加工产品中转基因成分的定量检测[J].大豆科学,2007,26(06):939-942.[doi:10.3969/j.issn.1000-9841.2007.06.026]
　WU Ming-sheng,TIAN Lei,KANG Jun-mei,et al.QUANTITATIVE DETECTION OF TRANSGENIC COMPONENTS IN PROCESSED SOYBEAN PRODUCTS[J].Soybean Science,2007,26(06):939-942.[doi:10.3969/j.issn.1000-9841.2007.06.026]
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大豆加工产品中转基因成分的定量检测

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年06期 页码: 939-942 栏目: 出版日期: 2007-12-25
Title:
QUANTITATIVE DETECTION OF TRANSGENIC COMPONENTS IN PROCESSED SOYBEAN PRODUCTS
文章编号:
1000-9841(2007)06-0939-04
作者:
吴明生; 田雷; 康俊梅; 贾希海; 律宝春
北京市种子管理站检验科，北京 100088
Author(s):
WU Ming-sheng; TIAN Lei; KANG Jun-mei; JIA Xi-hai; Lv Bao-chun
Beijing Seed Administration Station，Beijing 100088
关键词:
转基因大豆; 定量检测; PCR
Keywords:
Transgenic soybean; Quantitative Detection; PCR
分类号:
R155
DOI:
10.3969/j.issn.1000-9841.2007.06.026
文献标志码:
A
摘要:
根据转基因大豆的内源基因Lectin和外源基因CaMV35S的序列设计特异引物和探针，利用这些引物和探针，采用先定性后定量的方法对6份大豆加工产品转基因成分进行了定量检测。结果显示有两份样品含有转基因成分，其转基因成分含量分别为1.4％和0.4％。
Abstract:
According to the DNA sequences of endogenous Lecin gene and exogenous CaMV35S gene，primers and probes was designed.Using the primers and probes，transgenic components of six unknown samples were qualitatively detected followed by quantitatively detected.The results showed only two samples of them were positive and the content of transgenic components was 1.4% and 0.4%，respectively.

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备注/Memo

作者简介：吴明声（1978- ），男，农艺师，硕士，主要从事转基因产品检测和检测技术研究。E-mail:wumish2002@yahoo.com.cn; Tel: 010-62382454

更新日期/Last Update: 2014-10-19