[1]尹学哲,沈明花,全吉淑,等.高效液相色谱法测定大豆胚轴中单糖链大豆皂甙的含量[J].大豆科学,2007,26(06):943-945.[doi:10.3969/j.issn.1000-9841.2007.06.027]
　YIN Xue-zhe,SHEN Ming-hua,QUAN Ji-shu,et al.DETERMINATION OF MONODESMOSIDE SOYASAPONINS FROM SOYBEAN HYPOCOTYL BY HPLC[J].Soybean Science,2007,26(06):943-945.[doi:10.3969/j.issn.1000-9841.2007.06.027]
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高效液相色谱法测定大豆胚轴中单糖链大豆皂甙的含量

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年06期 页码: 943-945 栏目: 出版日期: 2007-12-25
Title:
DETERMINATION OF MONODESMOSIDE SOYASAPONINS FROM SOYBEAN HYPOCOTYL BY HPLC
文章编号:
1000-9841(2007)06-0943-03
作者:
尹学哲1; 沈明花1; 全吉淑1; 工藤重光2
1. 延边大学医学院，延吉 133000；
2.日本东北食效科学研究所，青森 030-0842
Author(s):
YIN Xue-zhe1; SHEN Ming-hua1; QUAN Ji-shu1; KUDOU Shigemitsu2
1.Medical College of Yanbian University，Yanji 133000；
2.Institute of Food Factors Science for Health，Aomori 030-0842，Japan
关键词:
大豆胚轴; 单糖链大豆皂甙; 高效液相色谱法
Keywords:
Soybean Hypocotyl; Mono-desmoside Soyasaponins
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.06.027
文献标志码:
A
摘要:
大豆皂甙具有多种药理作用，而对大豆制品中各类皂甙的定量分析是研究大豆皂甙药理作用的基础。采用高效液相色谱-差示折光（HPLC-dRI）检测法测定单糖链大豆皂甙的含量。色谱条件为：ODS-AM-303色谱柱（YMC，4.6 mm×250 mm，5 μm），柱温40℃，含0.1%三氟乙酸的乙腈-水（40∶60）为流动相，流速1mL min-1。Ba、Bb、Bd、Be、αg和βg分别在2.24~11.2、2.35~11.8、1.58~7.92、2.01~10.1、1.38~6.88和1.62~8.12 μg范围内线性关系良好，平均回收率分别为92.5%、93.8%、94.1%、95.8%、93.4%和94.2%，RSD为3.22%、3.18%、4.01%、3.53%、4.07%和4.28%。该方法准确度高，重现性好，适用于大豆皂甙样品中各类皂甙的测定。
Abstract:
Soyasaponins have many pharmacological effects.The determination of soyasaponins contents is the basis of the pharmacological experiments.In the current experiment mono-desmoside soyasaponins in soybean sample were detected by a high performance liquid chromatographic-differential refractive index detective (HPLC-dRI) method.The chromatographic condition was as fellows：ODS-AM-303 column (YMC，4.6 mm 250 mm，5 μm) with the temperature of 40℃，acetonitrile-water (40∶60) containing 0.1% trifluoroacetic acid as mobile phase with the flow rate of 1 mL min-1.The method was proved to be linear in ranges of 2.24-11.2，2.35-11.8，1.58-7.92，2.01-10.1，1.38-6.88 and 1.62-8.12 μg for Ba，Bb，Bd，Be，αg and βg，respectively；the recoveries were 92.5%，93.8%，94.1%，95.8%，93.4% and 94.2% with RSD of 3.22%，3.18%，4.01%，3.53%，4.07% and 4.28% for Ba，Bb，Bd，Be，αg and βg，respectively.The method is accurate and reproducible，and suitable for the determination of mono-desmoside soyasaponins.

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备注/Memo

基金项目：国家自然科学基金资助项目（30360113）

作者简介：尹学哲(1962-)，男，博士，教授，主要研究方向为中草药活性成分分离及药理作用。

更新日期/Last Update: 2014-10-20