[1]芦春斌,周文,刘标.喂食转基因大豆对子代雄鼠生殖系统的影响[J].大豆科学,2013,32(01):119-123.[doi:10.3969/j.issn.1000-9841.2013.01.028]
　LU Chun-bin,ZHOU Wen,LIU Biao.Effects of Transgenic Soybean on Reproductive System in Male Mice[J].Soybean Science,2013,32(01):119-123.[doi:10.3969/j.issn.1000-9841.2013.01.028]
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喂食转基因大豆对子代雄鼠生殖系统的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年01期 页码: 119-123 栏目: 出版日期: 2013-02-25
Title:
Effects of Transgenic Soybean on Reproductive System in Male Mice
文章编号:
1000-9841(2013)01-0119-05
作者:
芦春斌1;周文1;刘标2
1.暨南大学 生命科学技术学院 发育与再生生物学系，广东 广州 510632；
2.环境保护部南京环境科学研究所，江苏 南京 210042
Author(s):
LU Chun-bin1; ZHOU Wen1; LIU Biao2
1.Department of Development and Regenerative Biology,College of Life Science and Technology,Jinan University,Guangzhou 510632,Guangdong;
2.Nanjing Institute of Environmental Sciences,Ministry of Environmental Protection,Nanjing 210042,Jiangsu,China
关键词:
转基因大豆; 小鼠; 睾丸系数; 精子质量; 病理
Keywords:
Transgenic soybean; Mice; Coefficient of testis; Sperm quality; Pathology
分类号:
S865.1
DOI:
10.3969/j.issn.1000-9841.2013.01.028
摘要:
亲代小鼠分别喂食转基因和非转基因大豆饲料,120 d后组内交配获得子一代小鼠。以转基因饲料喂食的亲代所繁殖的子代小鼠作为实验组,断乳后子鼠继续喂食转基因大豆饲料;对照组子鼠为亲代非转基因喂食组所繁殖,断乳后继续喂食非转基因大豆饲料。分别于喂食30、60、90、120 d取子代小鼠的睾丸称重并计算脏器系数,伊红法染色检测精子存活率与畸形率;制作石蜡切片HE染色后观察睾丸病理学变化。结果表明:与对照组相比,实验组小鼠睾丸系数、精子存活率与畸形率均无显著性差异;组织切片镜检未发现两组小鼠的睾丸出现明显病理损伤。实验结果说明转基因大豆饲料喂食120 d未对子代雄鼠精子质量和睾丸组织病理造成影响,表明亲代长期喂食转基因大豆饲料后无潜在遗传毒性及积累效应。
Abstract:
The parental mice were fed transgenic soybean or control diets for 120 days respectively.After mating of intramural group,the offsprings were divided into transgenic group(GM)and control group(CK)corresponding to their parents.The GM group was fed with transgenic diets while the CK group was fed conventional diets for 120 days after weaning.At 30，60，90 and 120 days of feeding,the coefficient of testis were weighed，the sperm viability and abnormality were analyzed,and the pathology of testis was examined after HE staining.There were no significant differences in testis coefficient and sperm quality between two groups.Additionally,no significant pathology damages were observed in testis and epididymis.Results suggest transgenic soybean have no potential reproductive toxicity and genetic additive effects on mice.

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备注/Memo

基金项目：转基因生物新品种培育专项（2011ZX08012-005）；广东省科技计划项目（2010B031600108）。

第一作者简介：芦春斌（1964-），男，副研究员，硕士生导师，研究方向为转基因与生物安全。E-mail:tcblu@jnu.edu.cn

更新日期/Last Update: 2013-02-20