[1]刘丽君,薛永国,赵贵兴,等.大豆亚麻酸相关基因的标记与验证[J].大豆科学,2007,26(06):847-852.[doi:10.3969/j.issn.1000-9841.2007.06.008]
　LIU Li-jun,XUE Yong-guo,ZHAO Gui-xing,et al.MAPPING AND VERIFICATION OF GENES RELATED TO LINOLENIC ACID CONTENT IN SOYBEAN[J].Soybean Science,2007,26(06):847-852.[doi:10.3969/j.issn.1000-9841.2007.06.008]
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大豆亚麻酸相关基因的标记与验证

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年06期 页码: 847-852 栏目: 出版日期: 2007-12-25
Title:
MAPPING AND VERIFICATION OF GENES RELATED TO LINOLENIC ACID CONTENT IN SOYBEAN
文章编号:
1000-9841(2007)06-0847-06
作者:
刘丽君; 薛永国; 赵贵兴; 张雷
黑龙江省农业科学院大豆研究所，哈尔滨 150086
Author(s):
LIU Li-jun; XUE Yong-guo; ZHAO Gui-xing; ZHANG Lei
Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086
关键词:
大豆; 亚麻酸; 分子标记
Keywords:
Soybean; Linolenic acid; Molecular marker
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.06.008
文献标志码:
A
摘要:
大豆油脂组分决定着大豆品质和加工特性，其中亚麻酸含量关系到大豆油加工的成本和大豆油的品质特性。以哈89016和黑河18作为杂交亲本创造遗传群体，利用BSA方法，找到与亚麻酸含量相关的标记Satt286，根据Satt286在大豆杂交后代群体中的池子个体带型和表型性状，计算得到控制亚麻酸位点的引物Satt286与控制低亚麻酸的基因连锁。分析结果表明，控制亚麻酸的位点和引物距离9.5cM；t测验分析Satt286引物标记分组的高、低亚麻酸个体的实际亚麻酸含量，得到的平均值分别为3.91%和6.36%，二者差异达到极显著水平，说明Satt286引物作为筛选低亚麻酸品种的标记是有效的。在哈89016和黑河18后代（F4）群体中挑选出亚麻酸含量最低个体13个和最高个体9个，这些个体的带型和表型性状对亚麻酸含量控制基因符合率为82%，这表明引物Satt286与控制亚麻酸的基因是真实连锁的，可以作为群体后代的筛选标记。利用实时定量荧光PCR技术分析了大豆哈89016和黑河18品种亚麻酸相关基因Fad3c的基因表达量，结果表明，在发育的籽粒中，低亚麻酸品种哈89016的Fad3c的基因转录量明显低于黑河18，证实了大豆亚麻酸Fad3c基因的表达量与品种亚麻酸含量的高低有关。
Abstract:
Oil composition is one of critical factors affecting the qualities and processing properties of soybean，and linolenic acid content is closely related to the processing cost and qualities of soybean oil. In the experiment，Ha 89016 and Heihe 18 were used as parents to develop F2 generations，and bulked segregant analysis (BSA)method was adopted to detect SSR markers conferring linolenic acid content. Satt286 was found to closely link with genes controlling linolenic acid content，with a genetic distance of 9.5 cM. The average content of lower and higher linolenic acid individuals differentiated by Satt286 was 3.91% and 6.36%，respectively，and their difference reached 0.01 significant level. From the filial 4 generation of hybridization between H89016 and Heihe 18，13 individuals with lower linolenic acid content and 9 with higher linolenic acid content were selected and the accuracy between molecular marker and phenotypic value of each individual reached 82%，which confirmed the primer Satt286 closely linked with genes controlling linolenic acid content and could be used to select individuals differed in linolenic acid content. The expression level of gene Fad3c，which was related to linolenic acid content，were analyzed by real-time quantitative PCR method between soybean Ha 89016(lower linolenic acid content）and Heihe 18 (higher linolenic acid content)，and results showed the transcription rate of Ha-89016 was significantly lower than that of Heihe18，which further confirmed that the expression level of the Fad3c gene was related to the final linolenic acid content in different soybeans.

参考文献/References:

［1］徐杰．不同品质类型大豆品种脂肪酸形成规律的研究[D]．东北农业大学硕士学位论文，2005．

[2]Cregan P B，Jarvik T. An integrated genetic linkage map of the soybean genome[J].Crop Science，1999,35(5):1464-1490.
[3]宛煜嵩，王珍，方宣钧，等．一张含有227个SSR分子标记的大豆遗传连锁图[J]．分子植物育种,2005，3（1）：15-20.
[4]陈庆山，张忠臣，刘春燕，等．应用Charleston×东农594重组自交系群体构建SSR大豆遗传图谱[J]．中国农业科学，2005，38（7）：1312-1316.
[5]Pirjo Tanhuanp，Alan Schulman.Mapping of genes affecting linolenic acid content in Brassica rapa ssp.oleifera.[J]. Molecular Breeding，2002,10：51-62.
[6]Schneider，KA，Brothers M E，Kelly J D. Marker assisted selection to improve drought resistance in common bean[J]. Crop Science，1997，37:51-60.
[7]Rahman S M，Kinoshita T，Anai，et al.Combining ability in loci for high oleic and low linolenic acids in Soybean[J].Crop Science，2001,41:26-29.
[8]杨柳,张彬彬，韩英鹏，等．大豆亚麻酸QTL的分析[J]．大豆科学，2006，25（3）：270-274.
[9]薛庆中，张能义，熊兆飞．应用分子标记辅助选择培育抗白叶枯病水稻恢复系[J]．浙江农业大学学报，1998，24(6)： 631-638.
[10]Song Q J，Shoemaker R C. A new integrated genetic linkage map of soybean[J]. Theoritical and Applied Genetics，2004，109:122-128.[11]Shah S，Xin Z，Browse J. Overexpression of the FAD3 desaturase Gene in a mutant of arabidopsis[J]. Plant Physiology，1997，114：1533-1539.

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备注/Memo

基金项目：国家863项目（2006AA102IF9）；国家973项目（2002CB111300）

作者简介：刘丽君(1958-)，女，研究员，从事大豆遗传育种及栽培学研究。E-mail:nkyssbd@126.com

更新日期/Last Update: 2014-10-19