LIU Wen-hao,LI Wei,CHEN Xiang-yan,et al.Cloning and Sequence Analysis of an ω-3 Fatty Acid Desaturase Gene from High-oil and Low-oil Soybean Cultivars[J].Soybean Science,2008,27(04):569-571.[doi:10.11861/j.issn.1000-9841.2008.04.0569]
ω-3脂肪酸脱氢酶在高、低油大豆品种中的克隆与序列分析
- Title:
- Cloning and Sequence Analysis of an ω-3 Fatty Acid Desaturase Gene from High-oil and Low-oil Soybean Cultivars
- 文章编号:
- 1000-9841(2008)04-0569-03
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- α-亚麻酸(ALA)被称为必需脂肪酸，对人体有一系列的保健作用。ω-3脂肪酸脱氢酶（FAD）催化亚油酸(LA)生成ALA。大豆种子中ALA含量较高，为了比较高、低油大豆ω-3FAD的序列差异，用RT-PCR方法从大豆品种鲁豆11和郑9525的未成熟种子中扩增GmFAD3C的cDNA，获得的cDNA长度为1174bp，编码380个氨基酸。在推测的氨基酸序列中发现19处差异，为研究GmFAD3C基因在高、低油大豆种质中的差异表达奠定了基础。
- Abstract:
- α-linolenic acid(ALA)is an essential fatty acid which has a lot of healthcare functions to human.In plants，ω-3 fatty acid desaturases(FAD)catalyze linoleic acid(LA)to form ALA.The seed oil of soybean contains high level of ALA.RT-PCR was used to amplify the cDNA of GmFAD3C from the cultivars of Ludou11 and Zheng 9525.The length of the acquired cDNA，containing 380 amino acids，was 1174bp.Nineteen different amino acids were detected in the amino acid sequences of GmFAD3C from Ludou11 and Zheng 9525.These differences will help us to research the differential expression of GmFAD3C between high-oil and low-oil soybean.
参考文献/References:
[1]卢定强，陈庶来，陈钧.二十碳五烯酸和二十二碳六烯酸的代谢和生理机能[J].江苏理工大学学报，1998,5：29-36.（Lu D Q，Chen S L ,Chen J.Metabolism and physiological function of docosahexaenoic acid and eicosapentaenoic acid[J].Journal of Jiangsu University of Science and Technology，1998，5：29-36.）
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]刘丽君,薛永国,赵贵兴,等.大豆亚麻酸相关基因的标记与验证[J].大豆科学,2007,26(06):847.[doi:10.3969/j.issn.1000-9841.2007.06.008]
LIU Li-jun,XUE Yong-guo,ZHAO Gui-xing,et al.MAPPING AND VERIFICATION OF GENES RELATED TO LINOLENIC ACID CONTENT IN SOYBEAN[J].Soybean Science,2007,26(04):847.[doi:10.3969/j.issn.1000-9841.2007.06.008]
[12]韩英鹏,孙明明,刘昊飞,等.GGE双标图法评价大豆重组自交系亚麻酸表现的稳定性[J].大豆科学,2014,33(05):776.[doi:10.11861/j.issn.1000-9841.2014.05.0776]
HAN Ying-peng,SUN Ming-ming,LIU Hao-fei,et al.Perform Stability of Linolenic Acid Content of Soybean Recombinant Intercross Line Evaluated by GGE Biplot[J].Soybean Science,2014,33(04):776.[doi:10.11861/j.issn.1000-9841.2014.05.0776]
备注/Memo
基金项目：山东省自然科学基金资助项目（Y2006D20）;山东省农业科学院青年科研基金资助项目（2005YQ007）。