Yang Shouping Zeng Weiying Duan Meiping Yu DeyueGai Junyi.SSR MARKER LOCATION OF MALE STERILE GENE OFNUCLEAR MALE STERILE MUTANT NJ89 - 1 IN SOYBEAN[J].Soybean Science,2006,25(04):344-347.[doi:10.11861/j.issn.1000-9841.2006.03.0344]
大豆雄性不育突变体 NJ89 - 1 核不育基因的SSR 标记和定位*
- Title:
- SSR MARKER LOCATION OF MALE STERILE GENE OFNUCLEAR MALE STERILE MUTANT NJ89 - 1 IN SOYBEAN
- Keywords:
- Soybean ; Male sterile gene ; SSR marker location
- 文献标志码:
- A
- 摘要:
- 组合 NJ89 - 1 不育株 ×南农 73 - 935 的 F 1 、F 1∶2 、F 2∶3 的遗传数据表明在组合 NJ89- 1 不育株 ×南农 73- 935 中 NJ89- 1 雄性不育性仍受一对隐性基因控制;随机选取组合 NJ89 - 1 不育株 ×南农 73- 935 的一个 F 1∶2 株行群体作为分子标记定位群体 , 对 NJ89 - 1 不育基因进行SSR标记定位,结果发现 Sat - 402 与 NJ89- 1 不育基因连锁, 参照 Song 等(2004)整合的大豆遗传连锁图谱 ,初步将 NJ89- 1不育基因定位于大豆 C2 遗传连锁群上 ,NJ89 - 1 不育基因与 Sat -402 的遗传距离为 9. 6cM 。
- Abstract:
- T he analysis of genetic data of F 1 , F 1∶2 and F 2∶3 of cross NJ89 - 1 sterile plant × Nannong73(935 showed that the male sterility of NJ89- 1 was still controlled by a single recessive nuclear gene. Onepopulation of a F 2 line of cross NJ89- 1 sterile plant ×Nannong73(935 was selected randomly to map themale sterile gene of NJ89- 1 through SSR markers. The results showed that Sat - 402 was linked to themale sterile gene of NJ89 - 1. According to the integrated soybean genetic linkage groups of Song et al.(2004), it was found that Sat - 402 was on the linkage group C2. Thus the male sterile gene of NJ89 - 1was preliminarily located on the linkage group C2. The linkage distance between Sat - 402 and the malesterile gene of NJ89- 1 was 9. 6cM.
参考文献/References:
1 Kaul M L. Male sterility in higher plants[ M] . M onograph onTheoretical Applied Genetics, 1988. Vol. 10. Springer-verlag.Berlin.
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备注/Memo
基金项目:国家 973 项目(编号:2004CB7206), 国家自然科学基金重大项目(编号:30490250), 长江学者和创新团队发展计划(PC-SIRT), 南京农业大学作物遗传与种质创新国家重点实验室开放基金课题(编号:ZW2004009)。作者简介:杨守萍(1967 -), 女, 副教授, 博士, 主要从事大豆遗传育种研究。E - mail:spyang @njau. edu. cn联系人:盖钧镒教授, 中国工程院院士, E- mail:sri@njau. edu. cn。