[1]王晓春.农杆菌介导的大豆体细胞胚遗传转化影响因子的研究*[J].大豆科学,2005,24(01):21-25.[doi:10.11861/j.issn.1000-9841.2005.01.0021]
　Wang XiaochunWang GangJi JingWang PingLiu Shangqian.THE FACTORS INFLUENCING GENETIC TRANSFORMATION SYSTEM INSOMATIC EMBRYOS OF SOYBEAN MEDIATED BY AGROB ACTERIUM[J].Soybean Science,2005,24(01):21-25.[doi:10.11861/j.issn.1000-9841.2005.01.0021]
点击复制

农杆菌介导的大豆体细胞胚遗传转化影响因子的研究*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年01期 页码: 21-25 栏目: 出版日期: 2005-02-25
Title:
THE FACTORS INFLUENCING GENETIC TRANSFORMATION SYSTEM INSOMATIC EMBRYOS OF SOYBEAN MEDIATED BY AGROB ACTERIUM
作者:
王晓春 1;  2;  3 　王 　罡 **1 　季 　静 1 　王 　萍 2 　刘尚前 3
(1. 天津大学农业与生物工程学院,天津 300072;2. 淮海工学院 海洋学院, 连云港 222005;3. 河北北方学院南校区农业科学系 ,河北宣化 075131)
Author(s):
Wang Xiaochun1; 2; 3　Wang Gang12　Ji Jing12　Wang Ping2　Liu Shangqian3
(1. Tianjin University of Agricultural and Biological Engineering Academy , Tianjin 300072;2. Huaihai Institute of Techndogy ,Marine School ,Lianyungang , 222005;3. Department of Agronomy , Hebei North University , Xuanhua 075131)
关键词:
大豆; 农杆菌介导; 体细胞胚; 遗传转化
Keywords:
Soybean ; Somatic embryos of soybean ; Mediated by Agrobacterium ; Genetic transformation
DOI:
10.11861/j.issn.1000-9841.2005.01.0021
文献标志码:
A
摘要:
用大豆体细胞胚为外植体, 以抗性体细胞胚筛选率为转化率的指标 ,研究了农杆菌介导的大豆体细胞胚遗传转化系统的几种影响因子。结果表明 ,用球形期的体细胞胚受伤处理作为转基因的受体、预培养 1. 5 天有利于转化 ;筛选不同的代数,转化率在 3 个月以前明显下降 ,而在 3 个月以后则基本稳定在 8%左右。
Abstract:
The factors influencing genetic transformation about somatic embryos of soybean Mediated byAgrobacterium tumefaciens had been studied. It showed that the suitable condition was determined as fol-lowed :injured treatment of somatic embryos of soybean , pre - culture one day and a half. The transforma-tion frequency decreased with the date of selection delayed in three months, The transformation frequencykept 8%after three months.

参考文献/References:

1　王景雪, 孙毅. 农杆菌介导的植物基因转化研究进展[ J] . 生物技术通报, 1999 1:7- 13.

2　周思军, 李希臣, 刘昭军, 等. 通过农杆菌介导法将Bt(CryIA)基因导入大豆[ J] .大豆科学, 2001, 20(3):157 - 162.
3　徐香玲, 高晶, 刘伟华, 等. Ti 质粒介导 B、t 、k - d 内毒蛋白基因转化大豆的初步研究[ J] . 大豆科学, 1997, 16(1):6 - 11.
4　周思军, 李希臣, 刘昭军, 等. 大豆农杆菌介导转化系统的优化研究[ J] . 东北农业大学学报, 2001, 32(4):313 - 319.
5　赵桂兰, 刘艳芝, 李俊波, 等. 影响农杆菌介导的大豆基因转化因素的研究[ J] .大豆科学, 2001, 20(2):84 - 88.
6　王罡, 季静, 王萍, 等. 大豆基因型对根癌农杆菌菌株敏感性的研 究[ J] . 遗传, 2002, 24(2):297 - 300.
7 　Finer JJ, A Nagasawa. Development of anembryogenic susben sionculture of soybean [ Glycinemax (L .)Merrill][ J] . Plant Cell Tis-sue Organ . Cult. 1988, 1(5):125- 136.
8 　Sato S ,C Newell , K Kolacz , LTredo J Finer et al. Stable transfor-mation via Particle bombsrdment in two different soybean regen-eration systems[ J] . Plant Cell ReP . 1993, 12:408- 413.
9　T rick HN , RD Dinkins, ER Santarem, et al. Recent aevances insoybean transformation[ J] . Plant Tissue Cult . Biotech . 1997, 3:9- 26.
10 Barwale UB, HR Kerns, JM Widholm . Plant regeneration fromcalluscultures of several soybean genotypes via embryo genesisand organ genesis[ J] . Planta 1986, 67:473- 481.
11 方宏筠. 黑核桃体细胞胚状体发生及基因转化系统的建立[ J] . 园艺学报, 2000, 27(6):406 - 411.
12 王晓春, 刘尚前, 季静, 等. 影响大豆体细胞胚萌发率的因素[ J] . 大豆科学, 2004, 23(2):151 - 154.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]赵莉莉,李喜焕,李文龙,等.MYB转录因子 AtPHR1 转化大豆研究[J].大豆科学,2013,32(02):154.[doi:10.3969/j.issn.1000-9841.2013.02.004]
　ZHAO Li-li,LI Xi-huan,LI Wen-long,et al.Genetic Transformation of Transcription Factor AtPHR1 into Soybean by Agrobacterium-Mediated Method[J].Soybean Science,2013,32(01):154.[doi:10.3969/j.issn.1000-9841.2013.02.004]
[12]张福丽,舒文涛,张怡,等.大豆整体子叶节再生体系的建立及应用于农杆菌介导遗传转化初探[J].大豆科学,2012,31(06):865.[doi:10.3969/j.issn.1000-9841.2012.06.001]
　ZHANG Fu-li,SHU Wen-tao,ZHANG Yi,et al.Cluster Bud Induction of Soybean Whole Cotyledonary Node and Application in Agrobacterium mediated Genetic Transformation[J].Soybean Science,2012,31(01):865.[doi:10.3969/j.issn.1000-9841.2012.06.001]
[13]宋丽雅,盖 燕,何聪芬.向大豆导入琉璃苣Δ6-脂肪酸脱氢酶基因的初步研究[J].大豆科学,2012,31(02):173.[doi:10.3969/j.issn.1000-9841.2012.02.003]
　SONG Li-ya,GE Yan,HE Cong-fen.A Preliminary Study on Transferring Δ6-Fatty Acid Desaturase Gene from Borago Officinalis into Soybean[J].Soybean Science,2012,31(01):173.[doi:10.3969/j.issn.1000-9841.2012.02.003]
[14]杨 喆,唐晓飞,张 颖,等.农杆菌介导不同基因型大豆品种子叶节遗传转化条件的研究[J].大豆科学,2012,31(02):188.[doi:10.3969/j.issn.1000-9841.2012.02.006]
　YANG Zhe,TANG Xiao-fei,ZHANG Ying,et al.Optimization on Genetic Transformation Conditions of Agrobacterium-mediated Cotyledonary-nodes from Different Genotype Soybeans[J].Soybean Science,2012,31(01):188.[doi:10.3969/j.issn.1000-9841.2012.02.006]
[15]闫瑞叶,李喜焕,李桂兰,等.植酸酶基因phyA转化大豆品种研究[J].大豆科学,2011,30(03):356.[doi:10.11861/j.issn.1000-9841.2011.03.0356]
　YAN Rui-ye,LI Xi-huan,LI Gui-lan,et al.Transgenic Soybean (Glycine max. L） Plants with phyA Gene Improved Phytase Activity[J].Soybean Science,2011,30(01):356.[doi:10.11861/j.issn.1000-9841.2011.03.0356]
[16]赵晓雯,吴芳芳,狄少康,等.农杆菌介导的大豆子叶节遗传转化技术流程及操作要点[J].大豆科学,2011,30(03):362.[doi:10.11861/j.issn.1000-9841.2011.03.0362]
　ZHAO Xiao-wen,WU Fang-fang,DI Shao-kang,et al.Technique flow and Key Operation Points of Agrobacterium-mediated Genetic Transformation of Soybean Cotyledonary Node[J].Soybean Science,2011,30(01):362.[doi:10.11861/j.issn.1000-9841.2011.03.0362]
[17]于洋,侯文胜,韩天富.农杆菌介导大豆遗传转化技术的研究进展[J].大豆科学,2010,29(04):696.[doi:10.11861/j.issn.1000-9841.2010.04.0696]
　YU Yang,HOU Wen-sheng,HAN Tian-fu.Approaches to Agrobacterium-mediated Transformation in Soybean[J].Soybean Science,2010,29(01):696.[doi:10.11861/j.issn.1000-9841.2010.04.0696]
[18]康小虎,欧阳青,吴存祥张玉满白羊年曹孜义蔡文启韩天富.转γ-生育酚甲基转移酶基因大豆的获得[J].大豆科学,2004,23(03):236.[doi:10.11861/j.issn.1000-9841.2004.03.0236]
　Kang Xiaohu,Ouy ang Qing,Wu CunxiangZhang YumanBai YangnianCao ZiyiCai WenqiHan Tianfu.TRANSFORMATION OF SOYBEAN WITH γ-TMT FROM BRASSICA OLERAEAFOR α-TOCOPHEROL ELEVATION[J].Soybean Science,2004,23(01):236.[doi:10.11861/j.issn.1000-9841.2004.03.0236]

备注/Memo

项目来源:本研究由“国家植物转基因技术研究开发与中试基地建设”专项课题(J99 - B - 001)资助

作者简介:王晓春(1972 -), 女, 讲师, 硕士, 主要从事作物栽培与遗传育种工作的研究。联系电话:0313 - 2111778 或 13180579055
通讯作者:王罡, 教授, 博士生导师。Wgtt2003@yahoo. com. cn

更新日期/Last Update: 2016-12-22