[1]康小虎,欧阳青,吴存祥张玉满白羊年曹孜义蔡文启韩天富.转γ-生育酚甲基转移酶基因大豆的获得[J].大豆科学,2004,23(03):236-238.[doi:10.11861/j.issn.1000-9841.2004.03.0236]
　Kang Xiaohu,Ouy ang Qing,Wu CunxiangZhang YumanBai YangnianCao ZiyiCai WenqiHan Tianfu.TRANSFORMATION OF SOYBEAN WITH γ-TMT FROM BRASSICA OLERAEAFOR α-TOCOPHEROL ELEVATION[J].Soybean Science,2004,23(03):236-238.[doi:10.11861/j.issn.1000-9841.2004.03.0236]
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转γ-生育酚甲基转移酶基因大豆的获得

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第23卷 期数: 2004年03期 页码: 236-238 栏目: 出版日期: 2004-08-25
Title:
TRANSFORMATION OF SOYBEAN WITH γ-TMT FROM BRASSICA OLERAEAFOR α-TOCOPHEROL ELEVATION
文章编号:
1000 -9841(2004)03 -0236 -03
作者:
康小虎1 ;  3欧阳青1 ;  2吴存祥1张玉满2白羊年1曹孜义3蔡文启2韩天富1
1 .中国农业科学院作物科学研究所, 北京100081 ;
2 .中国科学院微生物研究所, 北京100080 ;
3 .甘肃农业大学农学院, 兰州730070
Author(s):
Kang Xiaohu1 ;  3Ouy ang Qing1 ;  2Wu Cunxiang1Zhang Yuman2Bai Yangnian1Cao Ziyi3Cai Wenqi2Han Tianfu1
1 .Insti tute of Crop Science , The Chinese Academy of Agricul tural Sciences , Beij ing 100081 ;
2 .Inst itute of Microbiology , The Chinese Academy of Sciences , Beijing 100080 ;
3 .College of Agronomy , Gansu Agricultural Universi ty , Lanzhou 730070
关键词:
大豆; γ-TMT ; α-生育酚; 农杆菌介导; 子叶节; PCR
Keywords:
Soybean ; γ- TMT ; α- tocopherol; Ag robacterium -mediated co -t ransfo rmation ; Cotyledonarynode ; PCR
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2004.03.0236
文献标志码:
A
摘要:
以大豆子叶节为外植体, 利用γ-生育酚甲基转移酶基因(γ-TMT )的种子特异性表达载体p7S -TMTL , 通过农杆菌介导法转化大豆, 获得26 株抗卡那霉素再生植株。PCR 检测结果表明其中4 株为阳性, 初步证明γ-TMT 已整合到这4 株大豆的基因组中。
Abstract:
Transformation of soy bean with seed specif ic expression vector p7S -TMTL w as conducted throughAgrobacterium -mediated co -transformat ion .Twenty -six Kanamycin resistant plants were examined by PCRw ith specific primers .Four plants show ed posit ive signals , indicating that γ- TMT w as t ransfo rmed into thegenome of these four plants .

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备注/Memo

基金项目:国家自然科学基金(批准号:30270856)和国家重点基础研究发展计划(2002CB111301)资助。

作者简介:康小虎(1977 -), 男, 在读硕士研究生。

更新日期/Last Update: 2017-01-01