[1]袁鹰　刘德璞　王玉民　郑培和.卡那霉素对大豆生长的抑制及筛选试验研究[J].大豆科学,2003,22(04):261-263.[doi:10.11861/j.issn.1000-9841.2003.04.0261]
　YuanYing Liu Depu Wang Yumin Zheng Peihe.STUDY ON CRITICAL AND SCREEN SOYBEAN GROWOF KANAMYCIN[J].Soybean Science,2003,22(04):261-263.[doi:10.11861/j.issn.1000-9841.2003.04.0261]
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卡那霉素对大豆生长的抑制及筛选试验研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年04期 页码: 261-263 栏目: 出版日期: 2003-11-25
Title:
STUDY ON CRITICAL AND SCREEN SOYBEAN GROWOF KANAMYCIN
文章编号:
1000 -9841(2003)04 -0261 -03
作者:
袁鹰　刘德璞　王玉民　郑培和
吉林省农业科学院生物技术中心, 公主岭136100
Author(s):
YuanYing 　Liu Depu 　Wang Yumin 　Zheng Peihe
Jilin ProvinceKey Open Labor On Agora -Biotechnology , Gongzhuling 136100
关键词:
大豆; 抑制; 筛选
Keywords:
Soybean ; Kanamycin ; Screen ; Restrain
分类号:
S 565.101.2
DOI:
10.11861/j.issn.1000-9841.2003.04.0261
文献标志码:
A
摘要:
用3 个大豆品种作为研究材料, 研究了卡那霉素对种子萌发、子叶节培养、茎尖和组培苗生根的抑制效应。结果表明不同的外植体对卡那霉素的反应存在较大的差异, 用卡那霉素浸泡种子,浓度最好是1000ppm , 而种子在含卡那霉素培养基上萌发, 一般为100 -200 ppm 效果较好, 子叶节在生长培养时卡那霉素可采用50 -100ppm 。而茎尖、子叶节组培苗, 一旦放入含有卡那霉素培养基内, 不论浓度多少, 无一生根。不同的基因型亦稍有差异。所以在筛选转化子之前一定要进行临界浓度的筛选试验。
Abstract:
The effects of kanamycin on seed germination , growth of cotyledon , rooting of shoots were investigated using 3soybean varieties.The results showed that the effect of kanamycin on different explants varied significantly.The critiealconcentration was 1000ppm .When soybeans seeds were soaked with kanamycin solution where as the cribical concentrationwas 50 -100ppm ,when soybeans were inoculated on media added kanamycin .For cotyledon node , the certain concentrationof kanamycin was 100ppm .None of shoot tips and shoots regenerated from cotyledon cuture could root even inthe media with lowest concentration of kanamycin .The effect of kanamycin showed slight on different genotypes .Thescreening for critical concentration of kanamycin must be done before the screening of transformants .

参考文献/References:

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备注/Memo

作者简介:袁鹰(1964-), 女, 副研究员, 从事植物组织培养及转基因研究。

更新日期/Last Update: 2016-12-31