Qiao Yake Li Guilan Wang Wenpo Gao Shuguo Bi Yanjuan Jing Yan.THE SALT TOLERANCE OF CALLUS OF WILD/CULTIVATED SOYBEANANDMDA CONTENTOF PLANT GROWN IN SALTY SOIL[J].Soybean Science,2003,22(02):0.
野生、栽培大豆愈伤组织耐盐性与植株盐胁迫下丙二醛(MDA)含量的关系
- Title:
- THE SALT TOLERANCE OF CALLUS OF WILD/CULTIVATED SOYBEANANDMDA CONTENTOF PLANT GROWN IN SALTY SOIL
- Keywords:
- Wild soybean ; Cultivated soybeans ; Callus ; Salt tolerance ; MDA
- 分类号:
- S 565.101
- 文献标志码:
- A
- 摘要:
- 以野生、栽培大豆共14 份材料的下胚轴作为外植体诱导愈伤组织, 在含不同浓度NaCl 的培养基上鉴定愈伤组织的耐盐性, NaCl 浓度(w/v)分别为0 %(CK), 0 .1 %, 0 .2 %, 0 .3 %, 0 .4 %,0 .5 %, 0 .6 %, 0 .7 %, 0 .8 %;14 份材料的幼苗在NaCl 为0 .3 %的盐土环境中生长25d 后, 测其幼苗的丙二醛(MDA)含量。试验结果表明:愈伤组织水平上, 栽培大豆品种中黄4 、通农5 、吉林35 耐盐性较强, 不同材料之间愈伤组织的耐盐性有明显差异, 耐盐性强的可在0 .8 %NaCl 浓度下生长,耐盐性弱的只能忍受0 .4 %NaCl 浓度;本试验确定大豆愈伤组织耐盐性鉴定适宜的浓度为0 .6 %。在盐胁迫下, 植株MDA 含量在0 .3 %NaCl 条件下均比在CK 土壤中高, 但随胁迫时间的延长而呈现不同的变化趋势。综合大豆植株MDA 含量的变化和愈伤组织的耐盐性之间, 并未发现相关性。
- Abstract:
- The 14 w ild soybeans and cultivated soybeans w ere studied in this experiment .Hypocotyls w ere usedas explants .Callus w as induced at MS +2 , 4 -D2 .0mg/L +6 -BA0 .5mg/L + sucrose 3 % + agar 0 .8 %.The salt tolerance of callus w as observed on different medium containing 0(as contrast), 0 .1 %, 0 .2 %, 0 .3 %,0 .4 %, 0 .5 %, 0 .6 %, 0 .7 %, 0 .8 %NaCl .The experimental materials were planted also in soil pond containing0 .0 % 、0 .3 %NaCl.The results showed that salt tolerance of callus from Zhonghuang 4 , Tongnong 5 and Jilin35w as st ronger .Among different materials , the difference of salt tolerance w as remarkable .The higher salt tolerancematerials could grow well o n medium containing 0 .8 %NaCl , the lower salt tolerance materials could onlyg row on medium containing 0 .4 %NaCl.Sui table concent ration for identifing salt tolerance of soybean callus was0.6 %NaCl in this experiment.MDA content of plant s in 0 .3 %NaCl w as higher than that in control.MDAcontent of plant s grow n on 0 .4 %NaCl soil pond at different time appeared dif ferent varies wi th the increase ofsalt st ress day s.The co rrelation between MDA content and callus salt tolerance w as not found in this paper .
参考文献/References:
1 余叔文, 汤章城主编.植物生理与分子生物学[ M] .北京:科学出版社, 2001 .
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备注/Memo
基金项目:河北省科技厅项目(99230313)。