[1]刘丽君　吴俊江　高明杰　蒲国峰　张淑珍 　邱丽娟 ?郑翠明 　谢华 　郝连林 　李玉征.大豆对SMV1 株系抗性基因的分子标记研究[J].大豆科学,2002,21(02):93-95.[doi:10.11861/j.issn.1000-9841.2002.02.0093]
　Liu Lijun Wu Junjiang Gao Ming jie Pu Guofeng Zhang Shuzhen Qiu LijuanZheng Cuiming Xie Hua Hao Lianlin LiYuzheng.PRELIMINARY STUDY ON IDENTIFICATION OF MOLECULARMARKER ASSOCIATED WITH RESISTANCE GENE TO SMV1[J].Soybean Science,2002,21(02):93-95.[doi:10.11861/j.issn.1000-9841.2002.02.0093]
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大豆对SMV1 株系抗性基因的分子标记研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第21卷 期数: 2002年02期 页码: 93-95 栏目: 出版日期: 2002-05-25
Title:
PRELIMINARY STUDY ON IDENTIFICATION OF MOLECULARMARKER ASSOCIATED WITH RESISTANCE GENE TO SMV1
文章编号:
1000 -9841(2002)02 -0093 -03
作者:
刘丽君　吴俊江　高明杰　蒲国峰　张淑珍 　邱丽娟 ?郑翠明 　谢华 　郝连林 　李玉征
黑龙江省农业科学院大豆研究所150086
Author(s):
Liu Lijun 　Wu Junjiang 　Gao Ming jie 　Pu Guofeng 　Zhang Shuzhen 　Qiu LijuanZheng Cuiming 　Xie Hua 　Hao Lianlin 　LiYuzheng
Soybean Research Inst itute , Hei longjiang Academy of Agricul tural Sciences , Harbin 150086
关键词:
大豆; 大豆SMV1 ; RAPD 技术
Keywords:
Soybean ; SMV1 ; RAPD
分类号:
S 565.035.3
DOI:
10.11861/j.issn.1000-9841.2002.02.0093
文献标志码:
A
摘要:
大豆花叶病毒病是世界大豆产区危害较重的主要病害, 本实验高抗SMV1 株系黑农39 为母本, 高感SMV1 株系品种合丰25 为父本配置杂交组合, 对F1 、F2 代接种SMV1 进行田间抗性鉴定和抗病性遗传学分析, 通过接种鉴定亲本F1 、F2 并代的抗性表现, 证明, 对SMV 1号株系的抗性是由一对显性基因决定的。利用RAPD 技术在抗感池间寻找多态性标记, 通过筛选引物, 获得重复性最好的随机引物OPN11 。并采用BSA 法在黑农39 和抗病池扩增出OPN1400片段, 在合丰25 和感病池扩增出OPN1300片段, 在F1 同时扩增出OPN1400和OPN1300 。用该引物分析黑农39 ×合丰25 的F2 扩增个体, 共显性的RAPD 标记OPN1400/1300与黑农39 抗病基因的遗传距离为8 .2cM 。
Abstract:
Soybean mosaic virus occurs w orldwide and it is a major soybean disease in China .Applying therandomly amplified Polymorphic DNA(RAPD)technique , w e have analy sed tw o soybean variet ies w hich w ereresistant and susceptible to SMV1 respectivly .We have demonst rated the inheritance of resistance of Heinong 39by studying the progenies of the cross of Heinong 39 X Hefeng 25 .We have identified one RAPD marker ,w hichw as OPN1400/ 1300 , closely linked to SMV resistance gene by BSA in F2 progenies of the cross w ith genetic distanceof 8 .2cM .

参考文献/References:

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5 　Poulsen D .M .E .The use of bulk segregant analysis t o identi fy aRAPD marker linked t o leaf rust resistance in barley 1995[ J] .Thero .Appl.Genet , 1995 , 91 :270 -273.
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备注/Memo

基金项目:黑龙江省杰出青年基金资助项目张淑珍, 东北农业大学大豆研究所博士研究生;邱丽娟;郑翠明;谢华, 在中国农科院品种资源所;郝连林, 现在黑龙江省林甸县花园乡农业站工作。李玉征在明水县种子公司。　

作者简介:刘丽君(1958 -), 女, 研究员, 从事大豆遗传育种工作。

更新日期/Last Update: 2016-12-30