[1]徐香玲　高晶　刘伟华　李集临.Ti质粒介导的B、t、k- δ内毒素蛋白基因转化大豆的初步研究[J].大豆科学,1997,16(01):7-11.[doi:10.11861/j.issn.1000-9841.1997.01.0007]
　Xu Xiang lingGao JingLiu WeihuaLi Ji lin.STUDIES ON TRANSFERRING B. t. k- DELTA ENDOTOXINGENE INTO SOYBEAN WITH Ti- PLASMID PRIMARIL[J].Soybean Science,1997,16(01):7-11.[doi:10.11861/j.issn.1000-9841.1997.01.0007]
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Ti质粒介导的B、t、k- δ内毒素蛋白基因转化大豆的初步研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第16卷 期数: 1997年01 页码: 7-11 栏目: 出版日期: 1997-02-20
Title:
STUDIES ON TRANSFERRING B. t. k- DELTA ENDOTOXINGENE INTO SOYBEAN WITH Ti- PLASMID PRIMARIL
作者:
徐香玲　高晶　刘伟华　李集临
(哈尔滨师范大学生物系　150080)
Author(s):
Xu Xiang ling　Gao Jing　Liu Weihua　Li Ji lin
( Harbin Normal Universi ty　150080)
关键词:
大豆;  Ti质粒;  B、t、k- δ内毒素蛋白基因;  再生植株
Keywords:
So ybean;  Ti - plasmid;  B. t. k- del ta endo toxin g ene;  Reg eneratedplant
DOI:
10.11861/j.issn.1000-9841.1997.01.0007
文献标志码:
A
摘要:
以Ti质粒为介导,将pKT54 B7 C5 质粒上的B、t、k- δ内毒素蛋白基因导入东北大豆“黑农37”、“黑农39”等品种。采用多种外植体和感染方法,从胚轴和子叶节诱导出丛生芽与再生植株。经卡那霉素筛选和冠瘿碱检测,初步证明外源基因导入大豆基因组中。共获得81株再生植株,其中成活30株,仅3株结7个荚,得到7粒种子。PCR检测和DN A分子杂交,鉴定这7株再生植株呈阳性反应,证明外源基因已导入并整合到大豆的基因组中。7粒种子均已萌发,植株生长发育正常。
Abstract:
The pKT54 B7 C5 plasmid wi th B. t. k. - del ta endoxin gene w ere t rnsferred intoHeino ng 37 and Heino ng 39 et. al soy bean cultiva rs by inducting wi th v arious ex plantmethods. Adv enti tio us buds a nd regenera tion plant w ere obtained f rom hy pocotyl ,cotylendo n node. They were detected by kanamycine selectio n and alkaoids and primarilyprov ed that fo reig n gene were t ransferred into so ybean genome. Only 30 o f 81 regeneratedpla nt surviv ed. Only 3 plants wi th 7 pods w ere obtained f rom the surviv ed regeneratedplant s. It prov ed that 7 plants of the reg enerated plant s w ere t ra nsferred w ithfo rg ein g ene by PCR technique a nd dot hybri zatio n. The 7 seeds spro uted, plant g row this normal.

参考文献/References:

[1 ]　田颖川等, 1991, “苏云金杆菌δ- 内毒素基因的转基因烟草的抗虫性”生物工程学报, 7( 1): 1- 10

[2 ]　李太元等, 1994, “高效抗虫转基因烟草的研究”中国科学B辑, 24( 3): 276- 282
[3 ]　施树良, 1994, 哈师大生物系硕士研究生论文“发根农杆菌Ri质粒介导二元载体向大豆转移PV Y- cp基因的研究”
[4 ]　许跃等, 1988, “酚类化物促进根癌农杆菌对植物离体外植体的高效转化”科学通报, 33( 2): 1745- 1785
[5 ]　李宝健, 1990, “应用农杆菌Ti质粒系统将外源基因导入禾山稻的研究”中国科学B辑, 2, 144- 149
[6 ]　Bart on. K. A. et al. , 1987 Pl an t Ph siol 85: 1103
[7 ]　Delannay. X. et. al. , 1989 Bio /Technology 7: 1265
[8 ]　Fis chh off . D. A. et al. , 1987 Bi o /Technol og y 5: 807- 813
[9 ]　Vacek. M. et al. , 1987 Nature 328: 33
[10 ]　"V tg ec Training coure manual" Universit y of Ts ukuba Gene Ex perinmen t 1991

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更新日期/Last Update: 2017-01-13