[1]赵现伟,赵朝森,王瑞珍,等.江西省大豆种质资源7S和11S球蛋白及其亚基相对含量分析[J].大豆科学,2019,38(04):503-510.[doi:DOI：10.11861/j.issn.1000-9841.2019.04.0503]
　ZHAO Xian-wei,ZHAO Chao-sen,WANG Rui-zhen,et al.Analysis of 7S and 11S Globulin and Their Subunits Relative Content of Soybean Germplasm in Jiangxi Province[J].Soybean Science,2019,38(04):503-510.[doi:DOI：10.11861/j.issn.1000-9841.2019.04.0503]
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江西省大豆种质资源7S和11S球蛋白及其亚基相对含量分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年04期 页码: 503-510 栏目: 出版日期: 2019-07-20
Title:
Analysis of 7S and 11S Globulin and Their Subunits Relative Content of Soybean Germplasm in Jiangxi Province
作者:
赵现伟1; 赵朝森1; 王瑞珍1; 郭兵福1; 王蓉蓉2; 曹小新3; 周金苟4
（1.江西省农业科学院 作物研究所/国家油料改良中心南昌分中心/江西省油料作物生物学重点实验室，江西 南昌 330200； 2.江西省瑞昌市种子管理局，江西 瑞昌 332200； 3.江西省九江市湖口县流芳乡农技站，江西 九江 332517； 4.江西省吉安市吉水县水南镇农技站，江西 吉安 343000）
Author(s):
ZHAO Xian-wei1;  ZHAO Chao-sen1;  WANG Rui-zhen1;  GUO Bing-fu1;  WANG Rong-rong2;  CAO Xiao-xin3;  ZHOU Jin-gou4
（1.Crops Research Institute of Jiangxi Academy of Agricultural Sciences/Nanchang Branch of National Center of Oilcrops Improvement/Jiangxi Province Key Laboratory of Oilcrops Biology, Nanchang 330200, China; 2.Administration of seeds of Ruichang City, Ruichang 332200, China; 3.Agricultural Technology Extension Station of Liufang Town, Jiujiang 332517, China; 4.Agricultural Technology Extension Station of Shuinan Town, Ji′an 343000, China）
关键词:
大豆; 种质资源; 7S球蛋白; 11S球蛋白; 亚基
Keywords:
Soybean;  Germplasm;  7S globulin;  l1S globulin;  Subunit
DOI:
DOI：10.11861/j.issn.1000-9841.2019.04.0503
文献标志码:
A
摘要:
为明确江西省大豆球蛋白各亚基相对含量及其比例，促进营养或加工品质优良大豆资源的挖掘与应用。本研究采用SDS-PAGE方法结合Gel-Pro Analyzer 4.5软件对供试的131份江西省大豆种质资源球蛋白及其亚基相对含量进行分析。结果表明：江西省大豆资源的7S、11S球蛋白及其亚基相对含量具有丰富的遗传变异，供试材料7S和11S球蛋白相对含量的变幅、平均值及变异系数分别为18.01%~52.21%、32.64%、19.46%和47.79%~81.99%、67.35%、9.43%，11S/7S比值范围为0.92~4.55，平均值为2.19，变异系数为30.64%，且筛选鉴定出3个11S/7S比值大于4.0和4个α′亚基、α亚基或β亚基含量较低的优异大豆种质资源，这些资源的挖掘为大豆品质的改良提供了材料和参考。
Abstract:
In order to clarify the relative content variation of globulin and their subunits, and then screening excellent germplasm to improve the nutritional and processing quality of soybean. This study analyzed 7S and 11S globulin and subunits content of 131 soybean accessions originated from Jiangxi province by SDS-PAGE(sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The study showed that the variation range, average and variable coefficient of relative content of 7S and 11S globulins were 18.01%-52.21%, 32.64%, 19.46% and 47.79%-81.99%, 67.35% and 9.43%, respectively. The variation of 11S/7S ration ranged from 0.92 to 4.55, and the average and variable coefficient of 11S/7S were 2.19 and 30.64%, respectively. The gathered data implied that soybean germplasm of Jiangxi province contains abundant genetic diversity of relative contents of 7S, 11S globulin and their subunits, among which 11S/7S ratios of 3 germplasm were higher than 4.0, and 4 accessions showed relative lower contents ofα′, αandβsubunits than others. This study provides a useful reference to improve high-quality breeding of soybean.

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备注/Memo

收稿日期：2019-12-04
基金项目：转基因生物新品种培育重大专项（2016ZX08004-005-009）；公益性行业（农业）科研专项（201503122-14）；国家现代农业产业技术体系专项（CARS-04-CES27）。
第一作者简介：赵现伟（1981-），男，硕士，助理研究员，主要从事大豆遗传育种、栽培技术与推广研究。E-mail: zhaoxianwei-2620@126.com。
通讯作者：王瑞珍（1958-），女，学士，研究员，主要从事大豆遗传育种、栽培技术与推广研究。E-mail: dadouzu@163.com。

更新日期/Last Update: 2019-07-25