[1]宁海龙,吴昊,李文滨,等.大豆四向重组自交系群体全生育期QTL的单标记分析[J].大豆科学,2015,34(06):1081-1084,1089.[doi:10.11861/j.issn.1000-9841.2015.06.1081]
　NING Hai-long,WU Hao,LI Wen-bin,et al.Single Marker Analysis on QTLs Controlling Maturity Period in Soybean Using A Four-way Recombinant Inbred Lines Population[J].Soybean Science,2015,34(06):1081-1084,1089.[doi:10.11861/j.issn.1000-9841.2015.06.1081]
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大豆四向重组自交系群体全生育期QTL的单标记分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年06期 页码: 1081-1084,1089 栏目: 出版日期: 2015-12-25
Title:
Single Marker Analysis on QTLs Controlling Maturity Period in Soybean Using A Four-way Recombinant Inbred Lines Population
作者:
宁海龙; 吴昊; 李文滨; 薛红; 李柏云; 李琦; 白雪莲; 李文霞
东北农业大学 农学院/大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030
Author(s):
NING Hai-long;  WU Hao;  LI Wen-bin;  XUE Hong;  LI Bai-yun;  LI Qi;  BAI Xue-lian;  LI Wen-xia
Agronomy College of Northeast Agricultural University/Key Laboratory of Soybean Biology in Ministry of Education/Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry, Harbin 150030, China
关键词:
大豆; 四向重组自交系群体; 全生育期; QTL; 优异等位基因型
Keywords:
Soybean;  Four-way recombinant inbred lines population;  Maturity;  QTL;  Excellent allelic genotype
DOI:
10.11861/j.issn.1000-9841.2015.06.1081
文献标志码:
A
摘要:
以含有160个株系的四向重组自交系为试验材料，通过188个SSR标记鉴定个体基因型，利用单标记分析方法，分析了2年4点试验获得全生育期表型数据，对该群体的全生育期进行了QTL 定位分析。结果表明：在 2013 和 2014 年哈尔滨、克山3个环境下定位了控制大豆全生育期的14个QTL，分别定位在大豆 20 个连锁群中的A2、B2、C1、C2、D1b、E、F、G、K、L、N共11个连锁群上，遗传率为1.34%～9.19%。可延长全生育期的优异等位基因型有BARCSOYSSR_08_0966（Q3Q3）、Sat_177（Q2Q2）、Sct_186（Q3Q3）、Satt307（Q3Q3）、Satt557（Q1Q1）、Satt577（Q2Q2）、Sat_351（Q1Q1）、Satt268（Q1Q1）、Sct_199（Q1Q1）、Satt273（Q3Q3）、Satt229（Q1Q1）、Satt664（Q1Q1）、Satt125（Q4Q4），能够缩短全生育期的优异等位基因型有BARCSOYSSR_08_0966（Q2Q2）、Sat_177（Q1Q1）、Sct_186（Q1Q1）、Satt307（Q2Q2）、Satt557（Q2Q2）、Satt577（Q4Q4）、Sat_351（Q2Q2）、Satt268（Q3Q3）、Sct_199（Q3Q3）、Satt273（Q2Q2）、Satt229（Q3Q3）、Satt664（Q3Q3）、Satt125（Q3Q3）。Satt307、Satt199和Satt125这3个QTL在2个环境重复检测到，其中Satt199在两个环境中的遗传率和等位基因的遗传效应差异较小，受环境条件的影响较小，可用于分子设计育种。
Abstract:
Abstract: In this paper a four-way recombinant inbred lines population with 160 individuals were used to map QTLs underlying the maturity period- 188 SSR primers were used to identify the genotype of the individuals. The maturity period was recorded in the 4 planting environments trails.Single marker analysis method was utilized to analyze the genotypic data and phenotypic data. The result showed that 14 QTLs conditioning maturity period were mapped to located in 11 linkage groups, i.e, A2, B2, C1, C2, D1b, E, F, G, K, L and N, and the heritability varied from 1.34% to 9.19%.The excellent allelic genotype that could delay the maturity include BARCSOYSSR_08_0966（Q3Q3）, Sat_177（Q2Q2）, Sct_186（Q3Q3）, Satt307（Q3Q3）, Satt557（Q1Q1）, Satt577（Q2Q2）, Sat_351（Q1Q1）, Satt268（Q1Q1）, Sct_199（Q1Q1）, Satt273（Q3Q3）, Satt229（Q1Q1）, Satt664（Q1Q1）, Satt125（Q4Q4）, and those that could shorten the maturity include BARCSOYSSR_08_0966（Q2Q2）, Sat_177（Q1Q1）, Sct_186（Q1Q1）, Satt307（Q2Q2）, Satt557（Q2Q2）, Satt577（Q4Q4）, Sat_351（Q2Q2）, Satt268（Q3Q3）, Sct_199（Q3Q3）, Satt273（Q2Q2）, Satt229（Q3Q3）, Satt664（Q3Q3）, Satt125（Q3Q3）. Among three QTLs i.e, Satt307, Satt199 and Satt125, which were detected in two environments simultaneously, Satt199 showed little variation in effects of allelic genotypes and heritability across two environments, which showed Satt199 could be used in molecular design breeding.

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备注/Memo

基金项目：黑龙江省教育厅科学技术研究重点项目（12541z001）；黑龙江省博士后科研启动基金（LBH-Q12152）；黑龙江省博士后科研启动基金（LBH-Q09165）。

第一作者简介：宁海龙（1975-），男，教授，博导，主要从事作物遗传育种与数量遗传研究。E-mail: ninghailongneau@126.com。
通讯作者：李文霞（1974-），女，副教授，硕导，主要从事作物遗传育种研究。E-mail: wxlee2006@ yahoo.com。

更新日期/Last Update: 2016-01-07