HENG Lin-jing,YAN Jun-hui,ZHONG Yun-peng,et al.Research to the Effective Methods of Soybean Somatic Embryo Induction and Proliferation[J].Soybean Science,2014,33(03):305-310.[doi:10.11861/j.issn.1000-9841.2014.03.0305]
大豆高效体细胞胚诱导和增殖方法的研究
- Title:
- Research to the Effective Methods of Soybean Somatic Embryo Induction and Proliferation
- Keywords:
- Genotype; Somatic embryo; Induction rate; Subculture
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以10个不同基因型的大豆幼胚为材料进行组织培养，研究不同播种季节和基因型对体细胞胚诱导率的影响，以及继代培养基状态和蔗糖含量对体细胞胚增殖的影响。结果表明：（1）同一基因型大豆幼胚，不同播种季节对体细胞胚诱导率的影响表现为春季>温室>秋季>夏季；（2）在同一培养和诱导条件下，体细胞胚诱导率较高的基因型是垦丰23，北4217，绥农30和绥农28；（3）液体的继代培养基相对固态，更有利于体细胞胚增殖；（4）继代培养基中高浓度蔗糖（60g.L -1）有利于体细胞胚的增殖。
- Abstract:
- With 10 genotypes of soybean immature embryos obtained from different seasons,the effects of different planting seasons and genotypes on somatic embryogenesis induction were primarily studied.And in order to optimize the regeneration system of soybean somatic embryos,the impact of liquid and solid subculture medium and the concentration of sucrose on the proliferation of somatic embryos were further researched.The result showed that(1)For the same genotype of soybean immature embryos,the effect of different planting conditions on somatic embryogenesis induction rate was spring>greenhouse>autumn>summer.(2)In the same culture condition,the genotypes which possessed the higher embryo induction rate were Kenfeng 23,North 4217,Suinong 28 and Suinong 30.(3)Compared to solid culture,liquid culture was favor of the proliferation of somatic embryos.(4)Subculture medium containing high concentration of sucrose(60g. L-1)was conducive to the proliferation of somatic embryos.
参考文献/References:
[ 1 ] Trick H N ,Finer J J. SAATdiated transformation [J] .329-336.sonication-assisted Agrobacterium-me- Transgenic Research . 1997 , 6 ( 5 ) :
[ 2 ] Hinchee M A , Connor-Ward D V , Newell C A , et al. Production oftransgenic soybean plants using Agrobacterium-mediated DNAtransfer[J] . ~ature Biotechnology , 1988 ,6 ( 8 ) :915 -922.
[ 3 ] Maughan P , Philip R, Cho M J . et al. Biolistic transformation . ex-pression, and inheritance of bovine p-casein in soybean ( Glycinemax ) [J] . In Vitro Cellular & Developmental Biology-Plant , 1999 , 35 ( 4 ) :344-349.
[ 4 ] Zhang Z, Xing A , Staswick P ,et al. The use of glufosinate as a se-lective agent in Agrobacterium-mediated transformation of soybean[J] . Plant Cell , Tissue and Organ Culture ,1999 ,56 ( I ) :37 -46.
[ 5 ] Aragao F J L. Sarokin L, Vianna G R, et al. Selection of transgenicmeristematic cells utilizing a herbicidal molecule results in the re-covery of fertile transgenic soybean [ Glycine max ( L. ) Merril ] plants at a high frequency [J] . Theoretical and Applied Cenetics,2000 .101 ( 1-2) :1-6.
[ 6 ] Donaldson P .Simmonds D. Susceptibility to Agrobacterium tumefa-ciens and cotyledonary node transformation in short-season soybean[J] . Plant Cell Repofis ,2000 ,19 ( 5 ) :478 -484.
[ 7 ] Olhoft P M, Flagel L E, Donovan C M. et al. Efficient soybeantransformation using hygromycin B selection in the cotyledonary-node method [J] . Planta .2003 ,216 ( 5 ) :723 -735.
[ 8 ] Zeng P, Vadnais D ,Zhang Z, et al. Refined glufosinate selection inAgrobacterium-mediated transformation of soybean [ Glycine max( L. ) Merrill] [J] . Plant Cell Reports ,2004 ,22 ( 7 ) :478 -482.
[9 ] Paz M M . Martinez J C , Kalvig A B , et al. Improved cotyledonarynode method using an alternative explant derived from mature seedfor efficient Agrobacterium-mediated soybean transformation [J] . Plant Cell Reports ,2006 ,25 ( 3 ) :206-213.
[ 10 ] Christou P, McCabe D E ,Swain W F. Stable transformation of soy-bean callus by DNA-coated gold particles [J] . Plant Physiology ,1988 .87 ( 3 ) :671-674.
[II ] Finer J J , Mcmullen M D. Transformation of soybean via particlebombardment of embryogenic suspension culture tissue [J] . In VitroCellular & Developmental Biology-Plant , 1991 ,27 ( 4 ) : 175-182. [ 12 ] Lippmann B , Lippmann G. Induction of somatic embryos in cotyle-donary tissue of soybean , Glycine max L. Merr. [J] . Plant Cell Re-ports ,1984 ,3 ( 6) :215-218.
[ 13 ] Ranch J , Oglesby L, Zielinski A C , et al. Plant regeneration fromembryo-derived tissue cultures of soybeans[J] . In vitro Cellular &Developmental Biology , 1985 ,21 ( ll ) :653 -658.
[ 14 ] Finer J J , ~agasawa A. Development of an emhryogenic suspensionculture of soybean ( Glycine max Merrill ) [ J l Plant Cell, Tissueand Organ Culture ,1988 ,15 ( 2) :125-136.
[ 15 ] Hartweck L , Lazzeri P , Cui D , et al. Auxin-orientation effects on so- matic embryogenesis from immature soybean cotyledons J l Invitro Cellular & Developmental Biology , 1988 ,24 ( 8 ) : 821 -828.
[ 16 ] Hepher A , Boulter M . Harris N , et al. Development of a superficialmeristem during somatic embryogenesis from immature cotyledonsof soyhean ( Glycine max L. ) [J] . Annals of Botany ,1988 ,62 ( 5 ) :513 -519.
[ 17 ] Stewart Jr C N ,Adang M J , All J N , et al. (--.enetic transformation ,recovery, and characterization of fertile soybean transgenic for asynthetic Bacillus thuringiensis cryIAe gene [J] . Plant Physiology , 1996 ,112( 1) :121-129.
[ 18 ] Meurer C , Dinkins R. Redmond C . et al. Embryogenic response ofmultiple soybean [ Glycine max ( L. ) Merr. ] cultivars across threelocations [J] . In Vitro Cellular & Developmental Biology-Plant,2001 ,37 ( 1) :62-67.
[ 19] Santarem E R.Finer J J. rl’ransformation of soybean [ Glycine max( L. ) Merrill] using proliferative embryogenic tissue maintained onsemi-solid medium [J] . In Vitro Cellular & Developmental Biology- Plant ,1999 ,35 ( 6) :451-455.
[20 ] 彰东升 , 赵守边诱导梅花提早开花初探 [J] . 湖北园林 ,1986 ,125 -126. ( Peng D S , Zhao S B. Primary research of inducing win-tersweet to blossom in advance l Huhei Landscape, 1986, 125 -126. )
[ 21 ] 徐 ffi. . 晴 , 陆佩玲 , 于强 . 气候变化对植 l:tr 物物 YJJ 候影响的研究进展 [J] . 资源 f-I- 学 ,2004 .26 ( I ) :129-136. ( Xu Y Q, Lu P L, Y Q.The research of climate change influencing plant phenology [J] . Resource Science .2004 ,26 ( I ) : 129-136. ) 饫及迷迭香酸的积累的影响 [J] . 时珍国医国药 ,2010 ,21 ( 6 ) : 50. ( Li H W , Su X H,Dong; C M , et al. Effects of carbon , nitrogensources on growth and rosmarinic acid accumulation of rabdosiarubescen.s ( Henmsl. ) hara callus [J] . Lishizhen Medicine and Mate-ria Medica Research .2010 ,21 ( 6) :50. )
[ 23 ] Samoylov V , Tucker D , Parrott W. Soybean [ Glycine max ( L. ) Mer- rill] embryogenic cultures: the role of sucrose and total nitrogen content on proliferation[J] . In Vitro Cellular & Developmental Bi- ology-Plant,1998 .34 ( I ) :8-13.
[24 ] Singh R, Klein T,Mauvais C , et al. Cytological characterization of transgenic soybean [J] . Theoretical and Applied Genetics , 1998 ,96 ( 2 ) :319-324.
[ 25 ] Komatsuda T , Lee W . Oka S. Maturation and germination of somat- ic embryos as affected by sucrose and plant growth regulators in soybeans Glycine gracili.s Skvortz and Glycine max ( L. ) Merr. [J] . Plant Cell . Tissue and Organ Culture . 1992 ,28 ( I ) : 103-113.
[26 ] Finer J J. Apical proliferation [ Glycine max ( L. ) Merrill ] [J] 238-241. of embryogenic tissue of soybean Plant Cell Reports , 1988 , 7 ( 4 ) :
[27] 万文娟，韩烈保，曾会明．植物细胞悬浮培养影响因子研究 [J] . 生物技术通报 ,2005 (5 ) :11-15. ( Fang W J ,Han L B ,Zeng H M. Research advances in factors affecting establishment of plant cell suspension culture [J]. Biotechnology Bulletin, 2005 ( 5 ) : 11-15. )
相似文献/References:
[1]刘 京,刘建巍,韩天富,等.潮霉素作为筛选剂对大豆发状根诱导的影响[J].大豆科学,2013,32(04):449.[doi:10.11861/j.issn.1000-9841.2013.04.0449]
LIU Jing,LIU Jian-wei,HAN Tian-fu,et al.Effect of Hygromycin as a Screening Agent on the Induction of Soybean Hairy Roots[J].Soybean Science,2013,32(03):449.[doi:10.11861/j.issn.1000-9841.2013.04.0449]
[2]王 萍,李 倩,王 罡,等.基因枪法转化大豆未成熟子叶影响因素的研究[J].大豆科学,2013,32(06):735.[doi:10.11861/j.issn.1000-9841.2013.06.0735]
[3]闫春娟,宋书宏,孙旭刚,等.基因型肥料互作下大豆群体的时空分布特征[J].大豆科学,2014,33(03):340.[doi:10.11861/j.issn.1000-9841.2014.03.0340]
YAN Chun-juan,SONG Shu-hong,SUN Xu-gang,et al.Temporal spatial Distribution Characteristics of Soybean( Glycine max L.Merr.)Under Genotype fertilizer Interaction Condition[J].Soybean Science,2014,33(03):340.[doi:10.11861/j.issn.1000-9841.2014.03.0340]
[4]马岩松,刘鑫磊,栾晓燕,等.大豆胞囊线虫病抗性基因相关分子标记对杂交后代抗性的鉴定效率[J].大豆科学,2014,33(02):173.[doi:10.11861/j.issn.1000-9841.2014.02.0173]
MA Yansong,LIU Xinlei,LUAN Xiaoyan,et al.Identification Efficiency about Resistance to Soybean Cyst Nematode with Relative Molecular Markers in Hybrid Progeny[J].Soybean Science,2014,33(03):173.[doi:10.11861/j.issn.1000-9841.2014.02.0173]
[5]刘业丽,栾怀海,何 琳,等.不同基因型大豆NADHGOGAT活性动态规律研究[J].大豆科学,2014,33(02):199.[doi:10.11861/j.issn.1000-9841.2014.02.0199]
LIU Yeli,LUAN Huaihai,HE Lin,et al.NADH GOGAT Activities in Different Genotypes Soybean[J].Soybean Science,2014,33(03):199.[doi:10.11861/j.issn.1000-9841.2014.02.0199]
[6]陈李淼,田星星,单志慧,等.利用农杆菌介导法转化大豆子叶节的影响因素研究[J].大豆科学,2012,31(01):17.[doi:10.3969/j.issn.1000-9841.2012.01.005]
CHEN Li-miao,TIAN Xing-xing,SHAN Zhi-hui,et al.Optimization of the Factors Affecting Genetic Transformation of Soybean Cotyledonary Node Mediated by Agrobacterium tumefacines[J].Soybean Science,2012,31(03):17.[doi:10.3969/j.issn.1000-9841.2012.01.005]
[7]王凤敏,李涛,王运杰,等.影响农杆菌介导大豆子叶节遗传转化因素的研究[J].大豆科学,2011,30(04):557.[doi:10.11861/j.issn.1000-9841.2011.04.0557]
WANG Feng-min,LI Tao,WANG Yun-jie,et al.Assessment of Factors Affecting Soybean Cotyledonary-node Agrobacterium-mediated Genetic Transformation[J].Soybean Science,2011,30(03):557.[doi:10.11861/j.issn.1000-9841.2011.04.0557]
[8]张庆林,赵艳,张艳,等.不同基因型大豆愈伤组织对农杆菌EHA105的敏感性研究[J].大豆科学,2011,30(04):566.[doi:10.11861/j.issn.1000-9841.2011.04.0566]
ZHANG Qing-lin,ZHAO Yan,ZHANG Yan,et al.Sensitivity of Different Genotype Soybean Callus to Agrobacterium EHA105[J].Soybean Science,2011,30(03):566.[doi:10.11861/j.issn.1000-9841.2011.04.0566]
[9]吴帅,王志坤,蓝岚,等.引进大豆种质遗传转化适用基因型的筛选[J].大豆科学,2012,31(01):29.[doi:10.3969/j.issn.1000-9841.2012.01.007]
WU Shuai,WANG Zhi-kun,LAN Lan,et al.Screening of the Optimal Acceptor Genotypes in Introduced Soybean Germplasm[J].Soybean Science,2012,31(03):29.[doi:10.3969/j.issn.1000-9841.2012.01.007]
[10]闫帆,孙昕,翟莹,等.大豆胚尖再生体系的研究[J].大豆科学,2011,30(05):757.[doi:10.11861/j.issn.1000-9841.2011.05.0757]
YAN Fan,SUN Xin,ZHAI Ying,et al.Optimization on the Regeneration System of Soybean Embryonic Tips[J].Soybean Science,2011,30(03):757.[doi:10.11861/j.issn.1000-9841.2011.05.0757]
[11]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(03):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[12]王萍,钟影,王罡,等.大豆体细胞胚的诱导及对草甘膦耐性的研究[J].大豆科学,2012,31(01):152.[doi:10.3969/j.issn.1000-9841.2012.01.035]
WANG Ping,ZHONG Ying,WANG Gang,et al.Induction of Soybean Somatic Embryos and Their Tolerance to Glyphosate[J].Soybean Science,2012,31(03):152.[doi:10.3969/j.issn.1000-9841.2012.01.035]
[13]王晓春,王罡,季静,等.2,4-D对大豆体细胞胚胎发生及增殖的影响[J].大豆科学,2009,28(06):1113.[doi:10.11861/j.issn.1000-9841.2009.06.1113]
WANG Xiao-chun,WANG Gang,JI Jing,et al.Somatic Embryogenesis and Proliferation of Soybean Embryogenic Tissue Treated with 2，4-D[J].Soybean Science,2009,28(03):1113.[doi:10.11861/j.issn.1000-9841.2009.06.1113]
备注/Memo
收稿日期：2013-12-09