[1]谷春梅,韩玲玲,曲洪生,等.大豆胰蛋白酶抑制因子的分离纯化[J].大豆科学,2013,32(01):89-97.[doi:10.3969/j.issn.1000-9841.2013.01.021]
　GU Chun-mei,HAN Ling-ling,QU Hong-sheng,et al.Extraction and Purification of Soybean Trypsin Inhibitor[J].Soybean Science,2013,32(01):89-97.[doi:10.3969/j.issn.1000-9841.2013.01.021]
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大豆胰蛋白酶抑制因子的分离纯化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年01期 页码: 89-97 栏目: 出版日期: 2013-02-25
Title:
Extraction and Purification of Soybean Trypsin Inhibitor
文章编号:
1000-9841(2013)01-0089-04
作者:
谷春梅1; 韩玲玲1; 曲洪生1; 宋鑫秀1; 赵琳琳1; 潘姝1; 秦贵信2
1.吉林农业大学 食品科学与工程学院，吉林 长春 130118；
2.吉林农业大学 动物科学技术学院，吉林 长春 130118
Author(s):
GU Chun-mei1; HAN Ling-ling1; QU Hong-sheng1; SONG Xin-xiu 1; ZHAO Lin-lin1; PAN Shu1; QIN Gui-xin2
1.College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118;
2.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,Jilin,China
关键词:
大豆; 胰蛋白酶抑制因子; 分离; 提取; 纯化
Keywords:
Soybean; Trypsin inhibitor; Seperation; Extraction; Purification
分类号:
S816.4
DOI:
10.3969/j.issn.1000-9841.2013.01.021
摘要:
以脱脂豆粉为原料,经pH7.6磷酸溶液抽提、65℃热变性、硫酸铵分步沉淀等提取技术制备粗提液,之后再经过DEAE-52离子交换、亲和层析和葡聚糖凝胶过滤等纯化技术研究大豆胰蛋白酶抑制因子的分离纯化方法。结果表明,从脱脂豆粉中分离纯化的大豆胰蛋白酶抑制因子的比活力高达4 600 U.mg-1,提纯倍数为73.85。纯化的大豆胰蛋白酶抑制因子经SDS-PAGE电泳分析,呈现2条谱带,分子量分别为21.92和20.04 kDa,这2种蛋白均为大豆胰蛋白酶抑制因子。该法操作简便,分离纯化效果好,对大豆胰蛋白酶抑制因子的研究与生产有重要的参考价值。
Abstract:
In this paper,soybean meal was used as raw material.The crude extract of the soybean trypsin inhibitor(STI) was obtained by the extraction of phosphoric acid buffer(pH7.6),thermal denaturation(65℃)and ammonium sulfate precipitation.Then this extract was purified by DE-52 ion exchange,affinity chromatography and sephadex G-75 gel filtration.The results showed that the specific activity of soybean trypsin inhibitor reached 4 600 U·mg-1and purification factor of 73.85 was obtained.The purified STI gave two protein bands in SDS-PAGE electrophoresis.Its molecular weights were estimated to be? 21.92 and 20.04 kDa.Due to the ease and simpleness of the procedure and high efficiency,the method has an important reference value to research and production of soybean trypsin inhibitor.

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备注/Memo

基金项目：国家自然科学基金项目（31000769）。

第一作者简介：谷春梅（1976-），女，讲师，博士，硕士生导师，研究方向为食品营养与安全及营养代谢调控。E-mail:jjnong2008@126.com。
通讯作者：秦贵信（1956-），男，教授，博士生导师，研究方向为比较营养学。E-mail:qgx@jlau.edu.cn。

更新日期/Last Update: 2013-02-20