SUN Jia-yao,LI Zi-wen,CUI Tian-yu,et al.Dynamic Changes of Endogenous Hormones in Flower Organ Development of Soybean Sterile Lines[J].Soybean Science,2020,39(06):891-897.[doi:10.11861/j.issn.1000-9841.2020.06.0891]
大豆不育系花器官发育过程中内源激素动态变化研究
- Title:
- Dynamic Changes of Endogenous Hormones in Flower Organ Development of Soybean Sterile Lines
- Keywords:
- Soybean; Sterile lines; Floral organ; Endogenous hormones
- 文献标志码:
- A
- 摘要:
- 为探索内源激素在大豆质核互做雄性不育系育性调控中的作用，利用间接酶联免疫技术 (ELISA)测定并分析高(A1)、中(A2)、低(A3)3种异交率不育系及其同型保持系(B1、B2、B3)在花蕾发育过程(花芽、花蕾、成花)中内源激素含量的变化。结果显示:花芽期不育系花器官中GA3含量均低于同型保持系，低异交率不育系A3在3个时期GA3含量显著低于高、中异交率不育系A1和A2；成花期不育系IAA含量均低于同型保持系，中异交率不育系A2在花蕾期和成花期IAA含量显著高于A1和A3；成花期不同异交率不育系ABA含量均低于同型保持系，中异交率不育系A2在花蕾期和成花期ABA含量显著高于A1和A3；花芽和花蕾期不育系IPA含量高于同型保持系，低异交率不育系A3在3个时期IPA含量显著低于高、中异交率不育系A1和A2；大豆不育系IAA/ABA、GA3/ABA表现为下降趋势，且花芽、花蕾、成花时期不育系大于保持系，IPA/ABA不育系表现为上升趋势。结果表明GA3、ABA、IAA及IPA含量异常、多种激素间平衡关系被打破及比例失调都与大豆雄性不育的发生有关。研究结果能够为探究大豆质核互作雄性不育机制及优良品种的选育提供一定的理论基础与试验依据。
- Abstract:
- In order to explore the role of endogenous hormones in the regulation of fertility of soybean cytoplasmic male sterile lines,the changes of endogenous hormones in high (A1), medium (A2) and low (A3) sterility lines and their homologous maintainers (B1, B2, B3) were determined by indirect enzyme linked immunosorbent assay (ELISA).The results showed that the content of GA3 in blossom bud period of male sterile lines was lower than that of maintainer lines. In the three stages, the GA3 content of male sterile line A3 with low outcrossing rate was significantly lower than that of the male sterile line A1 and A2 with high and medium outcrossing rate. IAA content of male sterile lines in flowering period was lower than that of homotypic maintainer lines, the IAA content of A2 in male sterile line with outcrossing rate was significantly higher than that of A1 and A3 in bud and flower period. At the flowering period, the ABA content of sterile lines with different outcrossing rates was lower than that of homotypic maintainers, the ABA content of A2 in male sterile line with outcrossing rate was significantly higher than that of A1 and A3 in bud and flower period. The IPA content of sterile line in blossom bud and bud period was higher than that of homotypic maintainer,in the three stages, the IPA content of male sterile line A3 with low outcrossing rate was significantly lower than that of male sterile line A1 and A2 with high and medium outcrossing rate. The results of IAA/ABA and GA3/ABA in soybean sterile lines showed a downward trend, and sterile lines in flowering period were greater than that of the maintainer lines, but the IPA/ABA sterile lines were opposite. The results showed that the abnormal contents of GA3, ABA, IAA and IPA and the imbalance among various hormones were all related to the occurrence of soybean male sterility.The results provide a theoretical and experimental basis for the breeding of superior varieties and the mechanism of cytoplasmic male sterility in soybean.
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备注/Memo
收稿日期：2020-03-14