[1]刘香英,康立宁,田志刚,等.东北大豆品种贮藏蛋白7S和11S组分及其亚基相对含量分析[J].大豆科学,2009,28(06):985-989.[doi:10.11861/j.issn.1000-9841.2009.06.985]
　LIU Xiang-ying,KANG Li-ning,TIAN Zhi-gang,et al.Analysis of 7S and 11S Globulin and Subunit Content of Soybean in Northeast China[J].Soybean Science,2009,28(06):985-989.[doi:10.11861/j.issn.1000-9841.2009.06.985]
点击复制

东北大豆品种贮藏蛋白7S和11S组分及其亚基相对含量分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年06期 页码: 985-989 栏目: 出版日期: 2009-12-25
Title:
Analysis of 7S and 11S Globulin and Subunit Content of Soybean in Northeast China
文章编号:
1000-9841(2009)06-0985-05
作者:
刘香英; 康立宁; 田志刚; 张井勇; 杨春明; 王景会; 姜媛媛; 张莉
吉林省农业科学院 农产品加工研究中心，吉林 长春 130033
Author(s):
LIU Xiang-ying; KANG Li-ning; TIAN Zhi-gang; ZHANG Jing-yong; YANG Chun-ming; WANG Jing-hui; JIANG Yuan-yuan; ZHANG Li
Research Center for Processing of Agricultural Products of Jilin Academy of Agricultural Sciences，Changchun 130033，Jilin，China
关键词:
大豆; 贮藏蛋白组分; 球蛋白; SDS-PAGE; 亚基
Keywords:
Glycine max（L.）; Storage protein; Globulin; SDS-PAGE; Subunit
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.06.985
文献标志码:
A
摘要:
利用聚丙烯酰胺凝胶电泳分析了黑龙江、吉林、辽宁和内蒙古4省（区）近10 a来通过审定的163个大豆品种的11S和7S球蛋白亚基组成、相对百分含量和11S/ 7S比值，并对其进行了省份间比较分析和相关性分析。结果表明：品种间大豆蛋白各亚基的相对含量存在较大差异；163份供试材料的7S、11S球蛋白平均相对含量分别为28.95% 和56.30%，变幅分别为18.40%~36.20%和47.90%~71.50%，11S/7S比值的变异幅度在1.34~3.32之间，平均值为1.97；初步筛选出α和A3缺失或稀少的特异大豆品种9份；吉林、黑龙江、辽宁和内蒙古4个地区大豆群体蛋白亚基组成及11S/7S比值存在显著差异。7S和11S组分含量间存在极显著的负相关(r=-0.2862，P<0.01）。东北大豆品种贮藏蛋白7S和11S组分及其亚基相对含量存在显著变异，为大豆品质育种和豆制品加工业原料选择提供了丰富的物质基础。
Abstract:
The contents of 7S and 11S globulins and their respective subunits，and the 11S/7S ratios were tightly associated with the nutrition and processing properties of soybean protein.The relative percentage content of 11S and 7S and the 11S/7S value of 163 soybean cultivars from Northeast China were investigated by SDS-PAGE method.Results showed the content of protein subunits varied greatly among varieties，the average content and variation rage of 11S and 7S globulin were 56.30% and 28.95%，47.90%-71.50% and 18.40%-36.20%，respectively；11S/7S value ranged from 1.34 to 3.32，with an average of 1.97；nine accessions screened from 163 germplasm showed polymorphism of band type for the subunit relative contents.11S/7S value was significantly different among the four cultivated regions；remarkably negative correlation existed between the relative contents of 7S and 11S globulins（r=-0.2862， P<0.01).Results suggest the subunit composition and their relative content of the storage protein 7S and 11S from Northeast China had remarkable variation，which would provid rich material for the soybean quality breeding.

参考文献/References:

[1]李富山，常汝镇，舒世珍，等.栽培、野生、半野生大豆蛋白质含量及氨基酸组成的初步分析[J].大豆科学，1986，5(1)：65-71.（Li F S，Chang R Z，Shu S Z，et al.Cultivated，wild，semi-wild soybean protein content and amino acid composition of the preliminary analysis[J].Soybean Science，1986，5(1)：65-71.）

[2]陈绍江，张国栋.大豆贮藏蛋白氨基酸组成与遗传密码的相关研究[J].东北农业大学学报，1994，25(2)：105-109 .(Chen S J，Zhang G D.Study on the relationship between amino acid composition and genetic code in soybean storage protein[J].Journal of Northeast Agricultural University，1994，25(2)：105-109.)
[3]刘至胜，李里特，辰巳英三.大豆蛋白质的营养品质和生理功能研究进展[J].大豆科学，2000，19(3)：263-266.(Liu Z S，Li Lite，Eiso Tatsumi.Nutritional quality and physiologucal functions of soy protein：A Review[J].Soybean Science，2000，19(3)：263-266.)
[4]张新会，杨晓泉.大豆蛋白摄入与冠心病关系的研究进展[J].粮食与油脂，2001(6)：6-9.(Zhang X H，Yang X Q.The relationship between soy protein and CHD[J].Food and Oil，2001(6)：6-9.)
[5]江和源，吕飞杰，邰建祥.大豆中生物活性成分及其功能[J].大豆科学，2000，19(2)：162-163.(Jiang H Y，Lv F J，Tai J X.Bioactive components of soybean and their fuction[J].Soybean Science，2000，19(2)：162-163.)
[6]Meng X X.Summarization about breeding of soybean qualification[J].Chinese Agricutrural Science Bulletin，1992，8(2)：14-17.
[7]Mori T，Utsumi S，Inaba H，et al.Differences in subunit composition of glycinin amang soybean cultivars[J].Journal of Agricultural and Food Chemistry，1981，29：20-23.
[8]Nieson N C.The structure and complexity of the 11S polypeptide in soybean[J].Journal of American Oil Chemists Society，1985，49：2733-2740.
[9]Staswick P E，Hermodson M A，Nielson N C.Identification of cystines which link the acidic and basic components of glycinin subunits[J].Journal of Biological Chemistry，1984，259：13431-13435.
[10]Kriboruchco D，Kaba H，Sambucetti M E，et al.Maturation time and some seed composition characters affecting nutritive value in soybean varieties [J].Cereal Chemistry，1979，56(4)：217-219.
[11]Murphy P A，Chen H P，Hauck C C，et al.Soybean storage protein composition and tofu quality [J].Food Technology，1997,51：86-88.
[12]Murphy P A，Resurreccion A P.Varietal and environmental differences in soybean glycinin and β-conglycinin content [J].Journal of Agricultural and Food Chemistry，1984，32：911-915.
[13]Cai T，Chang K C.Processing effect on soybean storage proteins and their relationship with Tofu quality [J].Journal of Agricultural and Food Chemistry，1999，47：720-727.
[14]Utsumi S，Kinsella J E.Structure-function relationships in food proteins：subunit interactions in heat-induced gelation of 7S，11S and soy isolate proteins [J] .Journal of Agricultural and Food Chemistry，1985，33（2)：297-303.
[15]姜振峰，陈庆山，杨庆凯，等.大豆种质资源贮藏蛋白亚基研究[J].东北农业大学学报，2006，37(5)：596-603.(Jiang Z F，Chen Q S，Yang Q K，et al.Analysis of subunits of storage protein of soybean germplasms resources[J].Journal of Northeast Agricultural University，2006，37(5)：596-603.)
[16]Wagner J R，Gueguen J.Surface functional properties of native，acid-treated，and reduced soy glycinin emulsifying properties [J].Journal of Agricultural and Food Chemistry，1999，47(6)：21812187.
[17]Teraishi M ，Takahashi M ，Hajika M，et al.Suppression of soybean β-conglycinin genes by a dominant gene，Scg-1[J].Theoretical and Applied Genetics，2001，103：1266-1272.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(06):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(06):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(06):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(06):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(06):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(06):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(06):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(06):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(06):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(06):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：吉林省农科院引进人才启动基金资助项目（00105）；吉林省科技发展计划资助项目（20070105）。

第一作者简介：刘香英(1981-) ,女,硕士,研究实习员，现主要从事大豆加工方面的研究工作。
通讯作者：康立宁,博士，副研究员。E-mail:lnkang @sina. com。

更新日期/Last Update: 2014-09-11