[1]蔡柏岩,葛菁萍,祖伟.磷素对不同大豆品种7S和11S球蛋白亚基积累的影响[J].大豆科学,2007,26(05):657-662.[doi:10.3969/j.issn.1000-9841.2007.05.004]
　CAI Bai-yan,GE Jing-ping,ZU Wei.EFFECT OF PHOSPHOROUS ON ACCUMULATION OF GLUTENIN 7S AND 11S FRACTIONS IN DIFFERENT SOYBEANS[J].Soybean Science,2007,26(05):657-662.[doi:10.3969/j.issn.1000-9841.2007.05.004]
点击复制

磷素对不同大豆品种7S和11S球蛋白亚基积累的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年05期 页码: 657-662 栏目: 出版日期: 2007-10-25
Title:
EFFECT OF PHOSPHOROUS ON ACCUMULATION OF GLUTENIN 7S AND 11S FRACTIONS IN DIFFERENT SOYBEANS
文章编号:
1000-9841(2007)05-0657-06
作者:
蔡柏岩1; 2; 葛菁萍1 ; 祖伟3
1黑龙江大学生命科学学院，分子生物学重点实验室，哈尔滨 150080；
2黑龙江东方学院，哈尔滨 150086；
3东北农业大学，哈尔滨 150030
Author(s):
CAI Bai-yan1; 2; GE Jing-ping1 ; ZU Wei3
1Key Laboratory of Molecular Biology，College of Life Science，Heilongjiang University，Harbin 150080；
2Heilongjiang East College，Harbin，150086;
3，Northeast Agricultural University，Harbin 150030
关键词:
大豆; 磷素水平; 球蛋白; 亚基含量
Keywords:
Soybean; Phosphorous level; Glutenin; Subunit content
分类号:
S565.101
DOI:
10.3969/j.issn.1000-9841.2007.05.004
文献标志码:
A
摘要:
选用东农42（高蛋白品种）、合丰25（中间型品种）、东农46（高油品种）3个基因型大豆品种作为试验材料，采用盆栽，在施N和K2O各为0.033 g kg－1土壤基础上，设P1、P2、P3、P4 4个P处理（分别施P2O50、0.033、0.067、0.100 g kg－1土壤），采用SDS-PAGE电泳法研究了不同磷素水平对大豆籽粒7S和11S球蛋白亚基积累和含量的影响，结果表明：不同品种不同处理间各种亚基出现后含量都是逐渐增加的，花后70 d达到最高峰，成熟期又开始下降。同一品种不同处理间东农42和合丰25两个品种7S、11S球蛋白各种亚基及球蛋白含量以P3处理含量最高，东农46以P2处理含量最高；同一处理不同品种间7S、11S球蛋白各种亚基及球蛋白含量始终是东农42最高，东农46含量最低，合丰25处于二者之间。施磷对3个大豆品种7S和11S球蛋白和亚基含量有影响，适宜的施磷量有利于提高球蛋白和亚基的含量。
Abstract:
Phosphorous plays an important part in the formation of glutenin subunits.The objective of the study was to investigate the effect of phosphorus on accumulation and content of glutenin 7S and 11S in different soybean varieties.Three widely distributed soybean genotypes in Heilongjiang Province，namely Dongnong 42(high-protein)，Dongnong 46(high-oil) and Hefeng 25(mid-type ) were used.The soybeans were planted in pots and fertilized with 0.033 g N kg-1soil and 0.033 g K2O kg-1soil.Four levels of phosphorous treatments，0，0.033，0.067，0.100 g P2O5kg-1 soil (denote as P1，P2，P3 and P4)were designed.The glutenin subunits，7S and 11S were extracted and analyzed by SDS-PAGE.The results showed that the contents of 7S and 11S subunits gradually increased，reached the peak values at 70 days after flowering and decreased at maturity.Each subunit content in 7S and 11S fractions as well as the glutenin contents reached their highest value under P3 treatment for Dongnong 42 and Hefeng 25;Under the same P treatment，the highest value of subunits contents of 7S and 11S and glutenin appeared in Dongnong 42，the lowest was in Dongnong 46.The results suggested that phosphorous treatment has an effect on the content of 7S and 11S as well as different subunits，and appropriate phosphorous treatment is beneficial to improve glutenin and subunits contents.

参考文献/References:

［1］王金龙，陈存来.大豆种子贮藏蛋白组份11S／7S研究概况[J].山东农业科学，1998，1：48－50.

[2]关荣霞，常汝镇，邱丽娟，等.栽培大豆蛋白亚基11S／7S组成及过敏蛋白缺失分析[J].作物学报，2004，30（11）：1076－1079.
[3] Koshiyama I.Chemical and physical properties in soybean glubulins[J].Cereal Chemistry，1968，45:394－404.
[4] Staswick P E，Hmodson M A，Nielsen N C.Identification of the acidic and basic subunit complex of glycinin[J].Journal of Biological Chemistry，1981，256:8752－8755.
[5]Kitamura K.Biochemical characterization of lipoxygenase lacking mutants，L1less，L2less，and L3less soybean[J].Agricultural and Biological Chemistry，1984，48(9):2339－2346.
[6]Larkins B A.Genetic engineering of seed storage proteins[J].Genetic Engineering of Plants，1983，45(5):93－119.
[7]郭顺堂，孟岩，张雪梅，等.中国大豆蛋白亚基构成分析与缺失部分亚基的特异大豆品种的筛选[J].作物学报，2006，32(8):1130－1134.
[8]Hill J E，Breidenbach R W.Protein of soybean seeds.Accumulation of the major protein components during seed development and maturation[J].Plant Physiology，1974，53:747－751.
[9]Beachy R N.Biosynthesis of subunits of the soybean 7S storage protein[J].Journal of Molecular and Applied Genetics，1981，1:19－24.
[10]郑易之，何孟元，郝水.大豆子叶蛋白体的形成与储藏蛋白质积累的关系[J].植物学报，1992，34(8):641－644.
[11]林忠平.利用免疫荧光法研究大豆种子发育过程中11Ｓ和7Ｓ蛋白的积累[J].中国科学(Ｂ辑)，1997，(3):285－288.
[12]Ochiai-Yanagi S，C.Fukazawa，K.Harada.Formation of storage protein components during soybean seed development[J].Agricultural and Biological Chemistry，1978，42:697－702.
[13]蔡柏岩，葛菁萍，祖伟.磷素水平对不同大豆品种氮素营养的影响[J].中国油料作物学报，2006，28（2）：156－161.
[14]雷勃钧.大豆属贮存蛋白的研究Ⅰ.大豆及某些野生类型大豆球蛋白构成的比较[J].大豆科学，1984，3（1）:36-39.
[15]许守民，苗以农，朱长甫，等.高低蛋白含量的大豆种子贮藏蛋白积累的比较研究[J].植物学报，1994，36（2）：111-115.
[16]徐豹.野生大豆（Glycine Soja）种子贮藏蛋白组份11S、7S的研究[J].作物学报，1990，16（3）：235-241.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(05):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(05):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(05):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(05):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(05):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(05):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]蔡柏岩,葛菁萍,祖伟.施磷水平对不同基因型大豆品种硝酸还原酶活性影响[J].大豆科学,2007,26(03):359.[doi:10.3969/j.issn.1000-9841.2007.03.015]
　CAI Bai-yan,GE Jing-ping,ZU Wei.THE AFFECTION OF PHOSPHOURS LEVEL TO DIFFERENT GENOTYPE SOYBEAN NITRATE REDUCTASE(NR)ACTIVITIES[J].Soybean Science,2007,26(05):359.[doi:10.3969/j.issn.1000-9841.2007.03.015]
[12]蔡柏岩祖伟葛菁萍.磷素水平对不同基因型大豆干物质积累与分配的影响[J].大豆科学,2004,23(04):273.[doi:10.11861/j.issn.1000-9841.2004.04.0273]
　[J].Soybean Science,2004,23(05):273.[doi:10.11861/j.issn.1000-9841.2004.04.0273]

备注/Memo

基金项目：黑龙江省教育厅资助项目（10543074）

作者简介：蔡柏岩（1968-），男，副教授，博士后，主要从事生态学研究。Tel：13936139957；E-mail：caibaiyan@126.com

更新日期/Last Update: 2014-10-20