[1]陈煜,朱保葛张敬梁宗锁.不同氮源对大豆硝酸还原酶和谷氨酰胺合成酶活性及蛋白质含量的影响[J].大豆科学,2004,23(02):143-146.[doi:10.11861/j.issn.1000-9841.2004.02.0143]
　Chen Yu,Zhu BaogeZhang JingLiang Zongsuo.EFFECTS OF DIFFERENT NITROGENS ON ACTIVITIES OF NITRATEREDUCTASE, GLUTAMINE SYNTHETASE AND SEED PROTEINVONTENTS IN SOYBEAN VULTIVARS[J].Soybean Science,2004,23(02):143-146.[doi:10.11861/j.issn.1000-9841.2004.02.0143]
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不同氮源对大豆硝酸还原酶和谷氨酰胺合成酶活性及蛋白质含量的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第23卷 期数: 2004年02期 页码: 143-146 栏目: 出版日期: 2004-05-25
Title:
EFFECTS OF DIFFERENT NITROGENS ON ACTIVITIES OF NITRATEREDUCTASE, GLUTAMINE SYNTHETASE AND SEED PROTEINVONTENTS IN SOYBEAN VULTIVARS
文章编号:
1000 -9841(2004)02 -0143 -04
作者:
陈煜1;  2朱保葛1张敬1梁宗锁2
1 .中国科学院遗传与发育生物学研究所, 北京100101 ;
2 .西北农林科技大学生命科学学院, 杨凌712100
Author(s):
Chen Yu1;  2Zhu Baoge1Zhang Jing1Liang Zongsuo2
1 .Inst itute of Genet ics and Developmental Biology , Chinese Academy of Sciences ,Bej ing , 100101 ;
2 .College of Life Sciences , Northwestern Sci -Tech University ofAgriculture and Forest ry , Yangling , 712100
关键词:
大豆; 硝酸还原酶; 谷氨酰胺合成酶; 蛋白质含量
Keywords:
Soybean ; Nit rate reductase ; Glutamine synthetase ; Seed protein content
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2004.02.0143
文献标志码:
A
摘要:
利用硝态氮(NO3--N)、铵态氮(NH4+ -N)及混合态氮对4 个栽培大豆品种结荚期植株进行诱导处理, 研究不同氮源对叶片硝酸还原酶(N R)、谷氨酰胺合成酶(GS)活性以及子粒蛋白质含量的影响。结果表明, NO3- -N 增加4 个品种NR 活性效果最好, 其次是混合态氮, 而NH4+-N 的效果较差。从4 个品种叶片的GS 活性看, 混合态氮和NH4+-N 的处理效果好, 而NO3--N 的效果差;三种氮源处理4 个基因型的子粒蛋白质含量都有不同程度的增加, 增加的顺序与提高NR 活性的顺序相同。说明施用三种氮肥能增加大豆品种子粒的蛋白质含量, 而以施用硝酸钾的效果最佳。相关分析表明, 大豆叶片N R 活性与子粒蛋白质含量之间呈极显著正相关(r =0 .663＊＊), 可以把结荚期叶片的硝酸还原酶活性作为选择高蛋白大豆材料的参考指标之一。
Abstract:
Effect s of different nitrogens(NO3--N ,NH4+ -N , NO3--NH4+)on nit rate reductase (N R)andg lutamine sy nthetase(GS)activities , and seed protein contents of soybean cultivars during pod -bearing periodw ere investigated .The experimental result s indicated that NR activities of leaves in four soybean geno typestreated by NO3- -N were obviously enhanced .However , NR act ivities of these genotypes treated by NH4+-N were the low est in three kinds of nit rogens .GS act ivit ies induced by NO3--NH4+and NH4+ -N w ere remarkablyincreased .Seed protein content s of four genoty pes induced by three kinds of ni trogens were higherthan those done by H2O(CK).The correlation between leaf NR activity and seed protein content w as ex tremelysignif icant (r = 0 .633＊).Hence it is suggested that high N R activity in soybean leaves should be one of bio -chemical index fo r selecting soybean germplasms wi th seed hig h protein .

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备注/Memo

基金项目:本工作得到国家“ 863” 计划项目(2001AA241061 -4 , 2002AA207007 , 2002AA211051),“ 973” 项目(2002CB111301)和中国科学院知识创新项目(KSCX2 -1 -01 -10)的部分资助。

作者简介:陈煜(1977 -), 女, 硕士研究生, 主要从事大豆生理生化研究。

更新日期/Last Update: 2016-12-31