[1]王文辉,邱丽娟常汝镇马凤鸣谢华林凡云.中国大豆种质抗SCN 基因rhg1 位点SSR 标记等位变异特点分析[J].大豆科学,2003,22(04):246-250.[doi:10.11861/j.issn.1000-9841.2003.04.0246]
　Wang WenhuiQiu LijuanChang RuzhenMa FengmingXie HuaLin Fanyun.CHARACTERISTICS OF ALLELES AT SATT 309 LOCUS ASSOCIATEDWITH rhg1 GENE RESISTANT TO SCN OF CHINESE SOYBEAN GERMPLASM[J].Soybean Science,2003,22(04):246-250.[doi:10.11861/j.issn.1000-9841.2003.04.0246]
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中国大豆种质抗SCN 基因rhg1 位点SSR 标记等位变异特点分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年04期 页码: 246-250 栏目: 出版日期: 2003-11-25
Title:
CHARACTERISTICS OF ALLELES AT SATT 309 LOCUS ASSOCIATEDWITH rhg1 GENE RESISTANT TO SCN OF CHINESE SOYBEAN GERMPLASM
文章编号:
1000 -9841(2003)04 -0246 -05
作者:
王文辉1;  2邱丽娟1常汝镇1马凤鸣2谢华1林凡云1;  3
1.中国农科院作物品种资源研究所, 农业部作物种质资源与生物技术重点开放实验室, 北京100081 ;
2.东北农业大学, 哈尔滨150030 ;3.西北农林科技大学, 杨凌712100
Author(s):
Wang Wenhui12Qiu Lijuan1Chang Ruzhen1Ma Fengming2Xie Hua1Lin Fanyun13
1.Key Lab of Crop Germplasm &Biotechnology , Ministry of Agriculture , Institute ofCrop Germplasm Resources , Chinese Academy of Agricultural Sciences , Beijing 100081 ;
2Northeast Agriculture University , Harbin , 150030 ;3Northwest Sci -Tech Universityof Agriculture and Forest , Yangling , 712100
关键词:
中国; 大豆(G .Max ); 胞囊线虫(SCN); rhg1 基因; 等位变异分布特点; Satt309
Keywords:
China ; Soybean (G .Max ); SCN ; rhg1 gene ; characteristic of alleles ; Satt309
分类号:
S 565.103.53
DOI:
10.11861/j.issn.1000-9841.2003.04.0246
文献标志码:
A
摘要:
利用与抗大豆胞囊线虫病主效基因rhg1 紧密连锁的SSR 标记Satt309 分析634 份中国大豆种质, 以21 份美国种质为对照, 目的是明确我国抗大豆胞囊线虫病种质资源的特点, 为大豆抗胞囊线虫病种质资源鉴定及抗线育种提供依据。149 份免疫或高抗种质和495 份感病种质共鉴定出6个等位变异, 其中介于134bp 和149bp 之间的Satt309 -5 为新等位变异, 具有该等位变异的陕西三股条黑豆和山西忻县小黑豆分别免疫和中抗1 号生理小种。对不同等位变异、不同抗性等级和抗不同生理小种种质的分析表明:92 .13 %的感病种质具有125bp 、131bp 或149bp 等位变异, 抗病种质在各等位变异都有分布, 但以128bp 和134bp 为主, 在具有这两个等位变异的种质中, 有72 .46 %表现为抗病, 且77 .27 %的免疫种质、87 .69 %的双抗种质和100 %的三抗种质都具有这两个等位变异。16 份具有128bp 等位变异的种质中有14 份来自山西省, 另有2 份是黑龙江省和河北省的育成品种, 推测山西省可能是Satt309 位点128bp 等位变异种质的起源地。本研究结果可为大豆抗胞囊线虫病育种的抗源选择提供一定的参考依据。
Abstract:
149 immune or high resistant accessions , 495 susceptible accessions and 21 accessions from U .S .A were analyzedusing SSR marker Satt309 which was closely linked to rhg1 , the most important gene resistant to SCN .A total of6 alleles were identified and the allele 5th between 134bp and 149bp was new , the two accessions with allele 5th wereimmune and medium resistant to Race1 separately .The results indicated that 92 .13 % susceptible accessions had one of125bp , 134bp and 149bp alleles ;while resistant accessions distributed at each allele , but most immune or multi -resistantaccessions were identified with 128bp or 134bp allele .14 of 16 accessions with 128bp allele came from ShanxiProvince , which suggested that Shanxi Province maybe was the original of accessions with 128bp allele .Results of thepaper can be references for the identification of resistant germplasm and breeding resistant to SCN .

参考文献/References:

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备注/Memo

基金项目:国家:“ 973” 项目(G1998010203)资助。　

作者简介:王文辉(1978-), 女, 硕士, 主要从事大豆抗胞囊线虫病种质研究。

更新日期/Last Update: 2016-12-31