大豆籽粒皂苷含量的全基因组关联分析
- Title:
- Genome-wide Association Analysis of Saponin Content in Soybean Seeds
- 摘要:
- 为解析大豆皂苷含量的遗传基础，本研究以包含264份大豆种质的自然群体为研究对象，利用高效液相色谱法（High Performance Liquid Chromatography，HPLC）检测3种大豆皂苷Aa（Soyasaponin Aa）、Ab（Soyasaponin Ab）和Bb（Soyasaponin Bb）的含量，再结合高密度大豆基因型数据进行全基因组关联分析。分析2020和2021年表型数据，结果显示大豆干籽粒中大豆皂苷Ab的平均含量最高，分别为0.311和 0.740 mg·g-1，大豆皂苷Aa和Bb的平均含量次之。相关性分析表明大豆皂苷Aa和大豆皂苷Ab显著负相关，大豆皂苷Ab与大豆皂苷Bb显著的正相关。全基因组关联分析发现，两年检测到的与大豆皂苷Aa和大豆皂苷Ab显著关联的SNP位点大多集中在7号染色体上，为主效QTL位点，并挖掘了调控大豆皂苷含量的4个候选基因；大豆皂苷Bb两年无共定位SNP位点，且显著关联的SNP较少，为4个。
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LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(03):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
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LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(03):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
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LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(03):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
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DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(03):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
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ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(03):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
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GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(03):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
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WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(03):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
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