LIU Si-tong,LIU Ya-hui,YIN Xue-zhe,et al.Comparation on the Effects of Soy Isoflavones and Vitamin E on Hepatic Antioxidant Capacity of Aging Mice[J].Soybean Science,2020,39(05):797-803.[doi:10.11861/j.issn.1000-9841.2020.05.0797]
大豆异黄酮和维生素E提高衰老小鼠肝脏抗氧化能力比较
- Title:
- Comparation on the Effects of Soy Isoflavones and Vitamin E on Hepatic Antioxidant Capacity of Aging Mice
- Keywords:
- Soybean; Isoflavones; Anti-aging; Antioxidant capacity; D-galactose
- 文献标志码:
- A
- 摘要:
- 为比较大豆异黄酮和维生素E对衰老小鼠肝脏抗氧化能力的作用，采用皮下注射D-半乳糖方法制备衰老模型小鼠，设置正常组、衰老模型组、维生素E组及大豆异黄酮组处理，采用ELISA法测定小鼠血清8-羟基脱氧鸟苷（8-OHdG）含量，比色法检测小鼠肝组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）、单胺氧化酶（MAO）、ATP酶的活性和丙二醛（MDA）含量，采用蛋白印迹法检测Nrf2和HO-1蛋白的表达，苏木素-伊红（HE）染色观察小鼠肝组织病理学改变。结果显示：衰老模型小鼠在大豆异黄酮和维生素E的作用下，血清8-OHdG含量均显著降低；肝组织中SOD、GSH-Px、CAT及线粒体ATP酶的活性均升高，MDA的含量及MAO的活性均显著降低；肝脏Nrf2和HO-1蛋白的表达水平均显著提高。表明大豆异黄酮和维生素E均可缓解衰老模型小鼠肝组织病变。除SOD、MAO活性及MDA含量，大豆异黄酮和维生素E组间其它指标均无显著差异，维生素E抗氧化能力强于大豆异黄酮，二者抗氧化机制可能与Nrf2和HO-1调控有关。
- Abstract:
- To compare the effects of soy isoflavones and vitamin E on hepatic antioxidant capacity of D-galactose-induced aging mice, we prepared the aging model mice by subcutaneous injection of D-galactose. The mice were randomly divided into 4 groups, including normal group, aging model group, vitamin E group and soy isoflavone group. Then the content of 8-hydroxy-2′-deoxyguanosine(8-OHdG) in serum was measured by ELISA. The activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT), monoamine oxidase(MAO), ATPase, and content of malondialdehyde (MDA) in liver tissues were detected by the colorimetric method. The protein expression of Nrf2 and HO-1 were detected with western blotting assay. The pathological changes of liver tissues were detected with hematoxylin-eosin(HE) staining. The results showed that soy isoflavones and vitamin E decreased 8-OHdG content in serum significantly. Soy isoflavones and vitamin E increased the activities of SOD, GSH-Px, CAT and mitochondrial ATPase significantly, decreased MAO activity and MDA content, and up-regulated the protein expression of Nrf2 and HO-1 in aging mice significantly. In conclusion, soy isoflavones and vitamin E alleviated structure damages of liver cells during aging. Except for SOD, MAO and MDA level, there was no significant difference in other indexes between soy isoflavone and vitamin E groups. Soy isoflavones and vitamin E could elevate the hepatic antioxidant capacity of aging mice induced by D-galactose, and the mechanism may be related to the regulation of Nrf2 and HO-1.
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