LIU Bei,QIU Shuang,HE Jia-qi,et al.Bioinformatics Analysis and Expression of Eight Dof Transcription Factors in Soybean Under Drought Stress[J].Soybean Science,2020,39(03):377-383.[doi:10.11861/j.issn.1000-9841.2020.03.0377]
8个大豆Dof转录因子的生物信息学分析及干旱诱导表达
- Title:
- Bioinformatics Analysis and Expression of Eight Dof Transcription Factors in Soybean Under Drought Stress
- 文献标志码:
- A
- 摘要:
- 为探究大豆Dof转录因子的功能，本研究对大豆中8个Dof转录因子的基因序列进行生物信息学分析，通过实时荧光定量PCR检测编码基因在干旱胁迫下的表达，并分析主要应答基因的启动子序列中的顺式作用元件。研究结果显示：8个Dof转录因子编码基因分别位于大豆5、8、11、13、15和16号染色体上，编码蛋白序列的氨基酸残基为213~403个，等电点为6.61~9.36，主要定位于细胞核中。GmDof2与番茄SlDof5.4具有较近的亲缘关系，GmDof1、GmDof8、GmDof3和GmDof5之间具有较近的亲缘关系，GmDof7与黄瓜CsDof1.4具有较近的亲缘关系，而GmDof4和GmDof6具有较近的亲缘关系。GmDof1和GmDof3的表达量在干旱胁迫下上升幅度最明显，启动子序列中均含有多种逆境相关的顺式作用元件。研究结果证明大豆Dof转录因子具有在植物抗旱基因工程中的应用前景。
- Abstract:
- In order to explore the functions of soybean transcription factors Dof, the bioinformatics analysis was performed on eight Dof genes in soybean. The expression levels of them were detected through real-time fluorescence quantitative PCR under drought stress, and the cis-elements in promoter sequences of major responsive genes were also analyzed. The results showed that the eight genes encoding the Dof transcription factors located on chromosome 5, 8, 11, 13, 15 and 16 of soybean, respectively. The length of their proteins ranged from 213 to 403 amino acid residues, and the isoelectric points ranged from 6.61 to 9.36, mainly located in the nucleus. GmDof2 was closely related to tomato SlDof5.4, GmDof1, GmDof8, GmDof3 and GmDof5 were closely related to each other, GmDof7 was closely related to cucumber CsDof1.4, and GmDof4 was closely related to GmDof6. The expression levels of GmDof1 and GmDof3 increased obviously under drought stress, and their promoter sequences both contained several cis-elements related to stress. The results showed that soybean Dof transcription factors have potential application in genetic engineering of plant drought resistance.
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