[1]王金生,王君,吴俊江,等.基于GGE-biplot的大豆耐低磷资源筛选[J].大豆科学,2018,37(04):511-516.[doi:10.11861/j.issn.1000-9841.2018.04.0511]
　WANG Jin-sheng,WANG Jun,WU Jun-jiang,et al.Screening of Soybean Germplasm Tolerant to Low Phosphorus Based on GGE-biplot[J].Soybean Science,2018,37(04):511-516.[doi:10.11861/j.issn.1000-9841.2018.04.0511]
点击复制

基于GGE-biplot的大豆耐低磷资源筛选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年04期 页码: 511-516 栏目: 出版日期: 2018-07-20
Title:
Screening of Soybean Germplasm Tolerant to Low Phosphorus Based on GGE-biplot
作者:
王金生1; 王君2; 吴俊江1; 刘庆莉1; 王树林1; 张鑫1
（1.黑龙江省农业科学院 大豆研究所/农业部大豆栽培重点实验室，黑龙江 哈尔滨 150086； 2.黑龙江省德强生物股份有限公司，黑龙江 哈尔滨 150060）
Author(s):
WANG Jin-sheng1; WANG Jun2; WU Jun-jiang1; LIU Qing-li1;  WANG Shu-lin1; ZHANG Xin1
（1.Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences/Key Laboratory of Soybean Cultivation，Ministry of Agriculture, Harbin 150086, China； 2.Deqiang Biology Company Limited in Heilongjiang Province,Harbin 150060, China）
关键词:
大豆; 耐低磷; GGE双标图
Keywords:
Soybean;  Tolerent to low phosphorus;  GGE-Biplot
DOI:
10.11861/j.issn.1000-9841.2018.04.0511
文献标志码:
A
摘要:
为了准确评价大豆耐低磷基因型在不同环境中的稳定性和适应性，采用GGE双标图，通过4种评价指标数据计算耐性因子GGE双标图数学模型对前期鉴定、评价获得的7个大豆耐低磷种质资源分别进行不同环境下耐低磷能力分析评价。结果表明：耐低磷性强且多环境下稳定性较好的品种为丰收24。以地下部干重计算耐性因子双标图显示垦鉴27表现出多环境下稳定的耐低磷性，而以地上部干重为评价指标则显示其耐低磷性较好但并不稳定；同样，以单株磷含量为评价指标显示克交05-1397同样表现出多环境下较稳定的耐低磷性，而以根系活跃吸收表面积评价指标显示其耐低磷性较好但不稳定。因此在利用GGE-biplot筛选耐低磷大豆资源时应结合具体的环境条件。研究结果对适于黑龙江地区不同环境条件下耐低磷大豆的应用具有重要的指导意义。
Abstract:
To accurately evaluate the stability and adaptability of the soybean genotype with resistance to low phosphorus in different environments, the aquired 7 tolerant soybean species were used to evaluate the ability of tolerance to low phosphorus in different environments by GGE double plot mathematical model method, which patience factor calculated thought four evaluation index data. The results showed that Fengshou 24 was the variety that with high tolerance to low phosphorus and stability in multi-environment. Kenjian 27 showed stable tolerance to low phosphorus in multi-environment according to underground dry weight while showed high but not stable tolerance according to overground dry weight. In the same way, with the content of single plant phosphorus as evaluation index, Kejiao 05-1397 showed stable tolerance to low phosphorus in the multi-environment, while it showed high but not stable tolerance with root activity surface area as evaluation index. Thus, it must be considerate the envirenment when screening low phosphorus resistant soybean resource. The results are with great guiding significance to the application of low phosphorus tolerant soybean in different environment in Heilongjiang.

参考文献/References:

［1］王艳，李晓林，张福锁．不同基因型植物低磷胁迫适应机理的研究进展［J］．生态农业研究，2000， 8（4）：34-36． (Wang Y, Li X L, Zhang F S. Current research on suitable mecllanisms on different plant genotypes under phosphorous stress［J］ .Eco-agriculture Research,2000,8(4):34-36.)
［2］李生秀．植物营养与肥料学科的现状与展望［J］．植物营养与肥料学报，1999，5（3）： 193-205．(Li S X. Current situation and outlook of plant nutrition and fertilizer science［J］.Plant Nutrition and Fertilixer Science,1999, 5 (3): 193-205.)
［3］赵华，徐森，石磊．植物根系形态对低磷胁迫应答的研究进展［J］．植物学通报，2006, 23（4）：409-417．（Zhao H, Xu S, Shi L. Advances in plant root morphology adaptability to phosphorous deficiency stress［J］.Chinese Bulletin of Botany, 2006, 23(4):409-417.)
［4］吴平，印莉萍，张立平，等．植物营养分子生理学［M］．北京：科学出版社，2001:103 -105．( W u P, Yin L P, Zhang L P, et al. Plant nutrition and molecule physiology［M］. Beijing Science Press,2001:103-105.)
［5］郭再华，贺立源，徐才国．低磷胁迫时植物根系的形态学变化［J］．土壤通报，2005，36（5）：760-763．( Guo Z H, He L Y, Xu C G.Morphological variety of plants tolerance to low P stress［J］.Chinese Joumal of Soil Science,2005, 36(5):760-763.)
［6］曹敏建，佟占昌，韩明祺．磷高效利用的大豆遗传资源的筛选与评价［J］．作物杂志，2001（4）：22-24．(Cao M J ,Tong Z C, Han M Q. Evaluation and filter on phosphate high active exploit of soybean inheritance resources［J］ . Crop Magazine,2001 (4):22-24.)
［7］曹爱琴，严小龙．不同供磷条件下大豆根构型的适应性变化［J］．华南农业大学学报，2000，22（1）：21．(Cao A Q, Yan X L. Adaptation of soybean root architecture under different P conditions［J］ .Journal of South China Agricultural University,2000,22(1):21.)
［8］丁洪，李生秀，郭庆元．酸性磷酸酶活性与大豆耐低磷能力相关研究［J］．植物营养与肥料学报，1997,3（2）:123-128．(Ding H, Li S X, Guo Q Y. Study on correlation between acid phosphase activity and low phosphorus tolerance of soybean［J］ .Plant Nutrition and Fertilizer Science, 1997, 3(2):123-128.)
［9］Gerloff G C, Gabelman W H. Genetic basis of inorganic plant nutrition［M］//Lauchli A,Bieleski R I. Encyclopedia of P lint physiology.Springer verlay Berlin,1983:453-480.
［10］王应祥，寥红，严小龙．大豆适应低磷胁迫的机理初探［J］．大豆科学，2003,22（3）:208-212．(Wang Y X, Liao H ,Yan X L. Prelimiminary studies on the mechanisms of soybean in adaption to low P stress［J］ .Soybean Science, 2003, 22(3):208-212.)
［11］王毅．植物耐低磷胁迫遗传学研究策略［J］．热带农业科学, 2004, 24(2):34-41．(Wang Y. Strategy genetics research on plant tolerant to low phosphorus［J］.Chinese Joumal Tropical Agriculture, 2004 ,24 (2):34-41.)
［12］Gabriel K R. The Biplot graphic display of matrices with application to principal component analysis［J］. Biometrika, 1971,58: 453-467.
［13］张宪政．植物生理学实验指导［M］．北京：中国农业出版社，2000:5-96．(Zhang X Z. Guide of phytophysiological experiment［M］.Beijing: China Agriculture Press,2000:5-96.)
［14］张志良．植物生理学实验指导［M］．北京：高等教育出版社，1990：5-8．(Zhang Z L. A guide of phytophysiological experiment ［M］. Beijing: High Education Press,2000:5-8.)
［15］邹琦．植物生理学实验指导［M］．北京：高等教育出版社,2000:59-60, 173-174．(Zou Q. Guide of phyto physiological experiment ［M］. Beijing: High Education Press, 2000:59-60,173-174.)
［16］李继云，刘秀娣，周伟，等．有效利用土壤营养元素的作物育种新技术研究［J］．中国科学（B辑），1995，25（1）：41-48．(Li J Y, Liu X D, Zhou W, et al..Studies on new breeding technology of efficiently utilizing soil nutritions［J］ Science in China (Series B), 1995, 25(1):41-48.)
［17］丁洪，李生秀，郭庆元.酸性磷酸酶活性与大豆耐低磷能力的相关研究［J］.植物营养与肥料学报, 1997，3（2）：123-128.(Ding H, Li S X, Guo Q Y. Study on correlation between acid phosphatese activity and low phosphorus tolerance of soybean ［J］.Plant Nutrition and Fertilizer Science, 1997, 3 (2):123-128.)
［18］黄亚群，马文奇，刘社平，等.春小麦品种磷效率相关性状的遗传［J］.河北省科学院报, 1999，16（2）：1-7.(Huang Y Q, Ma W Q, Liu S P, et al. Study on varieties of spring wheat inheritance of characters related to the phosphorus efficiency［J］. Journal of the Hebei Academy of Sciences,1999,16(2):1-7.)
［19］Vincent J M. A manual for the practical study of root-nodule ［M］. Oxford: IBP Handback, 1970:164．

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]武兆云,郭娜,赵晋铭,等.大豆苗期耐低磷主成分及隶属函数分析[J].大豆科学,2012,31(01):42.[doi:10.3969/j.issn.1000-9841.2012.01.010]
　WU Zhao-yun,GUO Na,ZHAO Jin-ming,et al.Principal Components and Membership Function Analysis of Low Phosphate Tolerance at Seedling Stage in Soybean[J].Soybean Science,2012,31(04):42.[doi:10.3969/j.issn.1000-9841.2012.01.010]
[12]王英,李喜焕,张彩英.河北大豆地方品种耐低磷种质筛选[J].大豆科学,2009,28(04):588.[doi:10.11861/j.issn.1000-9841.2009.04.0588]
　WANG Ying,LI Xi-huan,ZHANG Cai-ying.Screening of Low-P Tolerant Soybean Landraces from Hebei Growing-areas[J].Soybean Science,2009,28(04):588.[doi:10.11861/j.issn.1000-9841.2009.04.0588]
[13]赵艳红,陈怀珠,杨守臻,等.大豆耐低磷鉴定指标研究[J].大豆科学,2009,28(01):175.[doi:10.11861/j.issn.1000-9841.2009.01.0175]
　ZHAO Yan-hong,CHEN Huai-zhu,YANG Shou-zhen,et al.Identification Indexes of the Low phosphorus Tolerance of Soybean[J].Soybean Science,2009,28(04):175.[doi:10.11861/j.issn.1000-9841.2009.01.0175]
[14]刘海旭,吴俊江,王金生,等.大豆耐低磷研究进展[J].大豆科学,2017,36(04):639.[doi:10.11861/j.issn.1000-9841.2017.04.0639]
　LIU Hai-xu,WU Jun-jiang,WANG Jin-sheng,et al.Progress of Research on Tolerance to Low-phosphorus Stress in Soybean[J].Soybean Science,2017,36(04):639.[doi:10.11861/j.issn.1000-9841.2017.04.0639]

备注/Memo

收稿日期：2018-03-22

基金项目：国家重点研发计划课题（2017YFD0101306）；哈尔滨市科技创新人才研究专项基金（2013RFXYJ043）；国家现代农业产业技术体系（nycytx-004）。
第一作者简介：王金生（1981-），男，硕士，助理研究员，主要从事大豆耕作与栽培研究。E-mail:jinshengwang1981@163.com。
通讯作者：吴俊江（1970-），男，博士，研究员，主要从事大豆耕作与栽培研究。E-mail:nkywujj@126.com。

更新日期/Last Update: 2018-07-31