[1]郑淑波,于盛竹,赵洪锟,等.利用ISSR标记评价黑龙江和吉林小粒大豆品种遗传多样性[J].大豆科学,2018,37(03):347-352.[doi:10.11861/j.issn.1000-9841.2018.03.0347]
　ZHENG Shu-bo,YU Sheng-zhu,ZHAO Hong-kun,et al.Genetic Diversity of Small Seeded Soybean Varieties from Heilongjiang and Jilin Assessed by ISSR Markers[J].Soybean Science,2018,37(03):347-352.[doi:10.11861/j.issn.1000-9841.2018.03.0347]
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利用ISSR标记评价黑龙江和吉林小粒大豆品种遗传多样性

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年03期 页码: 347-352 栏目: 出版日期: 2018-05-20
Title:
Genetic Diversity of Small Seeded Soybean Varieties from Heilongjiang and Jilin Assessed by ISSR Markers
作者:
郑淑波1; 于盛竹2; 赵洪锟1; 袁翠平1; 刘晓冬1; 齐广勋1; 董英山1; 王玉民1
（1.吉林省农业科学院，吉林 长春 130033； 2.东北农业大学 资源与环境学院，黑龙江 哈尔滨 150030）
Author(s):
ZHENG Shu-bo1;  YU Sheng-zhu2;  ZHAO Hong-kun1;  YUAN Cui-ping1;  LIU Xiao-dong1;  QI Guang-xun1;  DONG Ying-shan1;  WANG Yu-min1
(1.Jilin Academy of Agricultural Sciences, Changchun 130033, China; 2.College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China)
关键词:
小粒大豆; 遗传多样性; ISSR标记
Keywords:
Small seeded soybean;  Genetic diversity;  ISSR marker
DOI:
10.11861/j.issn.1000-9841.2018.03.0347
文献标志码:
A
摘要:
为评价黑龙江省和吉林省小粒大豆品种的遗传多样性，从100个ISSR引物中筛选出多态性好的引物10个，对来自吉林省与黑龙江省的24份小粒大豆品种进行遗传多样性分析。结果表明：10个ISSR引物多态性信息含量为0.211 2~0.310 3，平均为0.249 6。ISSR标记指数为1.093 8~4.954 9，平均为2.890 3。24份小粒大豆的DICE相似系数为0.666 7~0.926 2，整体平均相似系数为0.794 3。吉林省小粒大豆品种之间的遗传相似系数范围（0.690 7~0.926 0）大于黑龙江小粒大豆品种之间的遗传相似系数范围（0.744 2~0.925 0）。聚类分析将供试材料分为4个类群，类群Ⅰ和类群Ⅱ均为吉林省小粒大豆品种，类群Ⅲ可以分为2个亚群。主坐标分析与聚类分析的结果基本一致。本研究结果可为小粒大豆遗传改良提供参考。
Abstract:
24 different small seeded soybean varieties from Jilin and Heilongjiang provinces were analyzed by 10 ISSR markers which were selected from 100 UBC ISSR primers. The result showed that the polymorphic information content of 10 ISSR primers ranged from 0.211 2 to 0.310 3 with an average of 0.249 6. The ISSR marker indexes ranged from 1.093 8 to 4.954 9, with an average of 2.890 3. The genetic similarity coefficient between 24 small seeded soybean varieties varied from 0.666 7 to 0.926 2 with an average of 0.794 3. The genetic similarity coefficient range (0.690 7-0.926 2) of small seeded soybean varieties from Jilin province was greater than that (0.744 2-0.925 0) of small seeded soybean varieties from Heilongjiang. The average similarity coefficient of small seeded soybean varieties (0.798 2) from Jilin was slightly lower than that of small seeded soybean varieties (0.798 9) from Heilongjiang. This result indicated that the genetic variation of small seeded soybean varieties from Jilin province was more abundant. 4 clustering groups were suggested by clustering analysis. The small seeded soybean varieties in GroupⅠand GroupⅡ were all from Jilin province, Group Ⅲ could be divided into 2 subgroups. The result of principle coordinate analysis was consistent with that of cluster analysis. The results in this study could provide reference for genetic improvement of small seeded soybean.

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备注/Memo

收稿日期：2017-08-15

基金项目：国家重点研发计划（2016YFD0100201）；吉林省农业科技创新工程重大项目（CXGC2017ZD014）。
第一作者简介：郑淑波（1988-），女，硕士，助理研究员，主要从事作物分子育种研究。E-mail: zhengshubo2010@126.com。
通讯作者：董英山（1963-），男，博士，研究员，主要从事大豆种质资源和大豆遗传转化研究。E-mail：ysdong@263.net；
王玉民（1968-），男，博士，研究员，主要从事大豆种质资源研究。E-mail: wangym@cjaas.com。

更新日期/Last Update: 2018-06-06