[1]孙晓环,刘晓冬,赵洪锟,等.吉林省龙井原位保护区野生大豆遗传多样性分析[J].大豆科学,2012,31(03):358-363.[doi:10.3969/j.issn.1000-9841.2012.03.005]
　SUN Xiao-huan,LIU Xiao-dong,ZHAO Hong-kun,et al.Genetic Diversity of Wild Soybean(G.soja)from Longjing in-situ Conserved Region of Jilin Province[J].Soybean Science,2012,31(03):358-363.[doi:10.3969/j.issn.1000-9841.2012.03.005]
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吉林省龙井原位保护区野生大豆遗传多样性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年03期 页码: 358-363 栏目: 出版日期: 2012-03-25
Title:
Genetic Diversity of Wild Soybean(G.soja)from Longjing in-situ Conserved Region of Jilin Province
文章编号:
1000-9841（2012）03-0358-06
作者:
孙晓环1; 2; 刘晓冬1; 赵洪锟1; 沈 波1; 王玉民1; 董英山 1
1.吉林省农业科学院 生物技术研究中心，吉林 长春130033；
2.黑龙江省农业科学院 牡丹江分院，黑龙江 牡丹江 157041
Author(s):
SUN Xiao-huan 1; 2;  LIU Xiao-dong1; ZHAO Hong-kun1; SHEN Bo1; WANG Yu-min1; DONG Ying-shan1
1.Biotechnology Research Center,Jilin Academy of Agricultural Sciences,Changchun 130033,Jilin;
2.Mudanjiang Branch,Heilongjiang Academy of Agricultural Sciences,Mudanjiang 157041,Heilongjiang,China
关键词:
野生大豆; SSR标记; 遗传多样性; 小种群; 原位
Keywords:
Wild soybean; SSR marker; Genetic diversity; Sub-population; In-situ conservation.
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.03.005
文献标志码:
A
摘要:
利用GPS对吉林省龙井原位保护区野生大豆定点采种，种群L（取样距离设计为50 m）覆盖整个龙井原位保护区。按天然地理分布人为分成A、B、C 3个小种群；同时，在小种群B中取小种群B’（包括32份材料，取样距离设计为18m）。聚类结果表明，90份材料主要划分为两大类群，其它材料亲缘关系较远。龙井原位保护区的野生大豆呈斑块分布，而且小种群间的遗传多样性与地理位置有一定的相关性。25个SSR位点的Simpson指数分布范围是0.3072～0.8393，平均值为0.6788；Shannon-weaver指数分布范围是0.5856～1.9348，平均值为1.3663，表明龙井原位保护区的野生大豆具有较高的遗传多样性。
Abstract:
Wild soybean accessions were collected from Longjing situ conserved region in Jilin province by GPS positioning.Population L,in which 90 wild soybean samples were collected(collection interval was 50 m)covered the whole in-situ conserved region.According to the natural geographical distribution,population L were divided into 3 sub-population A,B and C,respectively;meanwhile,a small sub-population B’(including 32 wild soybean samples,collection interval was 18 m)was taken from sub-population B.Clustering analysis shows that the 90 materials are mainly divided into 2 groups,and the others have a distant relationship,the distribution of the wild soybeans in Longjing conserved region was spotted,a correlation between the genetic diversity and geographical location were found among sub-populations.Simpson index ranged from 0.3072 to 0.8393,with an average of 0.6788.Shannon-weaver index ranged from 0.5856 to 1.9348,with a mean of 1.3663.These results indicated that there existed a highly genetic diversity in Longjing in-situ conserved population of Jilin province,and the genetic diversity of the three sub-populations have the same trend：A

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备注/Memo

基金项目：公益性行业（农业）科研专项经费资助；农业部野生资源保护与利用项目；国家自然基金青年基金项目（31000141）；吉林省自然科学基金项目（201215198）。

第一作者简介：孙晓环(1983-)，女，硕士，研究方向为大豆遗传育种。E-mail:xaozm@yeah.net。
通讯作者：王玉民（1968-），男，研究员，主要从事野生大豆种质资源及其利用研究。E-mail:wangym@cjaas.com。
董英山（1963-），男，研究员，主要从事植物种质资源和生物技术研究。E-mail:ysdong@cjaas.com。

更新日期/Last Update: 2014-08-16