[1]曾维英,梁江,陈渊,等.广西新收集野生大豆资源的遗传多样性分析[J].大豆科学,2011,30(03):379-383.[doi:10.11861/j.issn.1000-9841.2011.03.0379]
　ZENG Wei-ying,LIANG Jiang,CHEN Yuan,et al.Genetic Diversity Analysis of New Wild Soybean Collection in Guangxi[J].Soybean Science,2011,30(03):379-383.[doi:10.11861/j.issn.1000-9841.2011.03.0379]
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广西新收集野生大豆资源的遗传多样性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年03期 页码: 379-383 栏目: 出版日期: 2011-06-25
Title:
Genetic Diversity Analysis of New Wild Soybean Collection in Guangxi
文章编号:
1000-9841（2011）03-0379-05
作者:
曾维英1; 2; 梁江1; 陈渊1; 韦清源1; 汤复跃1; 钟开珍1; 陈文杰1
1.广西玉米研究所，广西 南宁 530227；
2.广西农业科学院 作物遗传改良生物技术重点实验室， 广西 南宁 530007
Author(s):
ZENG Wei-ying1; 2;  LIANG Jiang1;  CHEN Yuan1;  WEI Qing-yuan1;  TANG Fu-yue1;  ZHONG Kai-zhen1;  CHEN Wen-jie1
1.Guangxi Maize Research Institute, Nanning 530227；
2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
关键词:
广西; 野生大豆; 遗传多样性; SSR
Keywords:
Guangxi;  Wild soybean;  Genetic diversity;  SSR
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0379
文献标志码:
A
摘要:
利用23对SSR引物对广西新收集的200份野生大豆资源进行遗传多样性分析。结果表明：23个SSR位点共扩增出137个多态性带，每个位点等位变异数目范围为3~11个，平均为5.96个；Simpson指数分布范围为0.503~0.834，平均为0.689；Shannon-weaver指数分布范围为0.407~1.444，平均为0.683；遗传相似系数分布范围为0.266~0.969，平均为0.720，广西新收集野生大豆材料表现出丰富的遗传多样性。聚类分析结果显示，200份材料分为三大类，各地区材料之间存在着明显的遗传差异，但有些同一地区内的材料也表现出了明显的遗传分化。
Abstract:
Two hundred accessions of wild soybean, collected from Guangxi province were used as materials in this study. Twenty-three pairs SSR primers were used to analyze the genetic diversity of these materials. The result showed that one hundred and thirty-seven bands were amplified in twenty-three pairs SSR primers, with each locus has 5.96 bands. Genetic diversity index showed that distribution of Simpson index was 0.503-0.834, distribution of Shannon-weaver index was 0.407-1.444, and the dicesimilarity was 0.266-0.969， which suggestted that wild soybean collected from Guangxi had abundant genetic diversity. Clustering analysis showed that, two hundred accessions of wild soybean were divided into 3 categories. The genetic distance was very close among the materials in one population and also the materials of different populations because of their similar habitat. However, there were genetics differentiations in a same population.

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备注/Memo

基金项目：广西区青年科学基金资助项目(桂科青099171）；广西农业科学院基本业务费[200814(基)]；现代农业产业技术体系建设专项资金资助项目。

第一作者简介：曾维英（1981-），女，助理研究员，研究方向为大豆遗传育种与分子生物学。E-mail: zengweiying_1981@163.com。

更新日期/Last Update: 2014-09-11