[1]吴慧,高晓玲,陈琪,等.基于EST-SSR标记的黄淮海和南方大豆育成品种遗传多样性研究[J].大豆科学,2017,36(03):327-334.[doi:10.11861/j.issn.1000-9841.2017.03.0327]
　WU Hui,GAO Xiao-ling,CHEN Qi,et al.Genetic Diversity of Soybean Cultivars Released Based on EST-SSR Marker[J].Soybean Science,2017,36(03):327-334.[doi:10.11861/j.issn.1000-9841.2017.03.0327]
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基于EST-SSR标记的黄淮海和南方大豆育成品种遗传多样性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年03期 页码: 327-334 栏目: 出版日期: 2017-05-20
Title:
Genetic Diversity of Soybean Cultivars Released Based on EST-SSR Marker
作者:
吴慧; 高晓玲; 陈琪; 龚贵如; 熊冬金
（南昌大学 生命科学学院，江西 南昌 330031）
Author(s):
WU Hui;  GAO Xiao-ling;  CHEN Qi;  GONG Gui-ru;  XIONG Dong-jin
（College of Life Science Nanchang University, Nanchang 330031, China）
关键词:
大豆; 品种; EST-SSR 标记; 遗传多样性
Keywords:
Soybean;  Cultivar;  EST-SSR marker;  Genetic diversity
DOI:
10.11861/j.issn.1000-9841.2017.03.0327
文献标志码:
A
摘要:
以我国黄淮海和南方大豆产区的153份大豆育成品种为材料，选用26对EST-SSR分子标记通过Power Marker V 3-25等软件对其进行遗传多样性、相似性与特异性分析。结果表明：153份大豆共检测到238个等位变异，变幅3～25个，平均8.1个；多态信息量变幅0.15～0.87，平均0.61；遗传变异丰富。基于EST-SSR分子标记的聚类分析将153个材料聚为3大类13小类。特异性分析表明，黄淮海产区的育成品种的特有等位变异较南方产区的多，特缺等位变异要少于南方, 1991-2000年的特有等位变异最多；随着时间的推移，大量的外来育种材料应用于大豆育种，大豆育成品种的遗传基础有所拓宽。EST-SSR标记适用于大豆育成品种遗传多样性研究，研究结果可以为以后大豆种质资源保存与新品种的选育提供分子水平上的理论支持。
Abstract:
Study of the genetic diversity and the similarity among cultivars is important for crop genetic improvement.In this study, 26 EST-SSRs were investigated for the genetic diversity, similarity, and specificity of 153 soybean cultivars from Huang-Huai-Hai and Nan-Fang by Power Marker V 3.25.The results showed that there were wholly 238 alleles, averaged in 8.1 per locus, ranging from 3 to 25 with average polymorphism information content 0.61, ranging from 0.15 to 0.87 in the population.Based on EST-SSR marker data cluster analysis indicated that the 153 cultivars were clustered into 7 groups, 13 subgroups, with a tendency that the cultivars in the region inclined to be grouped into a same group.The genetic specificity analysis showed that Huang-Huai-Hai specifically existent alleles more rich alleles than the south, specifically deficient alleles less than the south.And specifically existent alleles the cultivars for the period of 1991-2000 were the most. Which result from some frequent germplasm exchange among the neighboring eco-regions and exotic breeding materials were applied in soybean breeding. The present results indicated that EST-SSRs markers were feasible and effective to analyze the genetic diversity of cultivated soybean and provide a significant reference for broadening the genetic base of soybean cultivars.

参考文献/References:

［1］熊冬金, 赵团结，盖钧镒. 中国大豆育成品种亲本分析［J］. 中国农业科学, 2008,41(9):2589-2598. (Xiong D J, Zhao T J, Gai J Y. Parental analysis of soybean cultivars released in China ［J］. Scientia Agricultura Sinica, 2008, 41(9): 2589-2598.)

［2］宋启建. 大豆SSR分子标记的创制及其应用［J］. 大豆科学, 1999, 18(3): 248-254.(Song Q J. A review of development and application of simple sequence repeat (SSR) in soybean［J］. Soybean Science, 1999, 18(3): 248-254.)
［3］王彪, 邱丽娟. 大豆SSR技术研究进展［J］. 植物学通报, 2002,19(1): 44-48.(Wang B, Qiu L J.Current advance of simple sequence repeats in soybean［J］. Chinese Bulletin of Botany, 2002, 19(1): 44-48.)
［4］Wang L X, Guan R X, Li Y H, et al. Genetic diversity of Chinese spring soybean germplasm revealed by SSR markers［J］. Plant Breeding, 2008,127: 56-61.
［5］徐立恒, 李向华.SSR标记对野生大豆种群遗传结构的研究［J］. 大豆科学, 2011, 30(1): 41-45. (Xu L H, Li X H. Analysis on genetic structure of wild soybeans populations by SSR markers［J］. Soybean Science, 2011,30(1): 41-45.)
［7］Qutob D, Gijzen M.Comparative analysis of expressed sequences in Phytophthora sojae.［J］. Plant Physiology, 2000, 123: 243-254.
［8］Song Q J, Marek L F, Shoemaker R C, et al. A new integrated genetic linkage map of the soybean［J］. Theoretical and Applied Genetics, 2004, 109(1): 122-128。
［9］Gai J, Chen L, Zhang Y, et al. Genome-wide genetic dissection of germplasm resources and implications for breeding by design in soybean［J］. Breeding Science, 2012, 61(5): 495-510.
［10］Varshney R K, Thiel T, Stein N, et al.In silico analysis on frequency and distribution of microsatellites in ESTs of some cereal species［J］. Cellular & Molecular Biology Letters, 2002, 7: 537-546.
［11］Thiel T, Michalek W, Varshney R, et al. Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley(Hordeum vulgare L)［J］. Theoretical and Applied Genetics, 2003, 106(3): 411-422.
［12］Gupta P K, Rustgis, Sharma S, etal.Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat［J］. Molecular Genetics and Genomics, 2003, 270: 315-323.
［13］Nicot N, Chiquet V, Gandon B, et al. Study of simple sequence repeat(SSR) markers from wheat expressed sequence tags(ESTs)［J］. Theoretical and Applied Genetics, 2004, 109: 800-805.
［14］杨新泉, 刘鹏, 韩宗福等. 普通小麦Genomic-SSR和EST-SSR分子标记遗传差异及其与系谱遗传距离的比较研究［J］. 遗传学报, 2005, 32(4): 406-416. (Yang X Q, Liu P, Han Z F, et al.Comparative analysis of genetic diversity revealed by genomic-SSR, EST-SSR and pedigree in wheat (Triticum asetivum L)］［J］. Acta Genetica Sinica, 2005, 32(4):406-416.)
［15］Hiroshi H, Shusei S, Sachiko I, et al. Characterization of the soybean genome using EST-derived microsatellite markers［J］. DNA Research, 2007, 14(6): 271-281.
［16］常玮, 赵雪, 李侠, 等. 大豆 EST-SSR标记开发及与Genomic-SSR的比较研究［J］. 中国油料作物学报, 2009, 31(2): 149-156. (Chang W, Zhao X, Li X, et al. Development of soybean EST-SSR marker and comparison with genomic-SSR marker［J］. Chinese Journal of Oil Crop Sciences, 2009, 31(2): 149-156)
［17］蔺宇, 徐静静, 王晓鸣, 等.适用于大豆疫霉菌遗传分析的新EST-SSR标记［J］. 中国农业科学, 2008, 41(8): 2294-2301.(Lin Y, Xu J J, Wang X M, et al.Novel EST-SSR markers for genetic analysis of Phytophthora sojae［J］. Scientia Agricultura Sinica, 2008, 41(8): 2294-2301.)
［18］Liu Y L, Li Y H, Zhou G A, et al. Development of soybean EST-SSR markers and their use to assess genetic diversity in the Subgenus Soja［J］. Agricultural Sciences in China, 2010, 9(10): 1423-1429.
［19］Zhang G, Xu S, Mao W, et al. Determination of the genetic diversity of vegetable soybean［Glycine max (L) Merr］ using EST-SSR markers［J］. Journal of Zheijang University, 2013, 14(4): 279-288.
［20］Saha M C, Mian M A R, Eujayl I, et al. Tall fescue EST-SSR markers with transferability across several grass species［J］. Theoretical and Applied Genetics, 2004, 109(4): 783-791.
［21］熊冬金, 王吴彬, 赵团结, 等. 中国大豆育成品种10个重要家族的遗传相似性和特异性［J］. 作物学报, 2014, 40(6): 951-964.(Xiong D J, Wang W B, Zhao T J, et al. Genetic similarity and specificity of ten important soybean cultivar families released in China［J］. Acta Agronomica Sinica, 2014, 40(6): 951-964).

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　HUANG Zhi-ping,ZHANG Lei,WANG An-dong,et al.BREEDING DEVELOPMENT STRATEGIES AND APPRAISAL OF SOYBEAN VARIETIES OF“WANDOU SERIES”[J].Soybean Science,2007,26(03):423.[doi:10.3969/j.issn.1000-9841.2007.03.028]
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　[J].Soybean Science,2004,23(03):289.[doi:10.11861/j.issn.1000-9841.2004.04.0289]

备注/Memo

基金项目：国家自然科学基金(31260332；30871550)。

第一作者简介：吴慧（1992-）,女，硕士，主要从事大豆种质资源研究。E-mail: 924749030@qq.com。
通讯作者：熊冬金（1962-），男，博士，副教授，主要从事大豆种质资源研究。E-mail: jxxdj@163.com。

更新日期/Last Update: 2017-06-24