[1]韩英鹏,赵雪,高赛男,等.大豆花叶病毒病N1 株系抗性基因定位分析[J].大豆科学,2016,35(03):407-410.[doi:10.11861/j.issn.1000-9841.2016.03.0408]
　HAN Ying-peng,ZHAO Xue,GAO Sai-nan,et al.Mapping of Gene with Ｒesistance to Soybean Mosaic Virus Strain N1[J].Soybean Science,2016,35(03):407-410.[doi:10.11861/j.issn.1000-9841.2016.03.0408]
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大豆花叶病毒病N1 株系抗性基因定位分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 407-410 栏目: 出版日期: 2016-05-20
Title:
Mapping of Gene with Ｒesistance to Soybean Mosaic Virus Strain N1
作者:
韩英鹏; 赵雪; 高赛男; 李海燕; 李文滨
东北农业大学农学院大豆生物学教育部重点实验室，农业部北方大豆生物学与遗传育种区域重点实验室，黑龙江哈尔滨150030
Author(s):
HAN Ying-peng; ZHAO Xue; GAO Sai-nan; LI Hai-yan; LI Wen-bin
Agronomy College，Northeast Agricultural University /Chinese Education Ministry’s Key Laboratory of Soybean Biology /Key Laboratory of NortheasternSoybean Biology and Breeding ( Genetics) of Chinese Agriculture Ministry，Harbin 150030，China
关键词:
大豆;  大豆花叶病毒病;  抗性基因定位;  分子辅助选择
Keywords:
Soybean;  Soybean mosaic virus;  Mapping of resistance gene;  Molecular assistant selection
分类号:
S511
DOI:
10.11861/j.issn.1000-9841.2016.03.0408
文献标志码:
A
摘要:
对大豆花叶病毒病N1 株系不同抗性材料东农93046( 抗) 与品1246( 感) 所衍生的F8∶9代群体进行成株抗性鉴定，同时利用分子标记对其抗性基因进行确认并获得用于分子辅助选择的分子标记。结果表明: F8∶9代抗病家系数与感病家系数基本符合1∶1 的比例，说明东农93-046 对N1 株系的成株抗性表现为质量性状，其抗性受一对等位基因控制。同时，根据前人研究结果利用76 个SSＲ 分子标记对父母本进行筛选，构建了一个包括13 个SSＲ 分子标记的F连锁群的遗传连锁图谱，并且单标记分析法和复合区间分析法的结果均表明东农93-046 抗性基因位点位于SSＲ 分子标记Satt114 附近，对后代群体中抗病和感病株系各60 份进行分子标记准确性评价，结果表明Satt114 选择准确率可达80. 00%，这为分子标记辅助选择奠定了良好的基础。
Abstract:
Soybean mosaic virus ( SMV) is one of the most widely distributed viral disease in soybean producing areas，whichseriously impact on soybean yield and quality. Molecular marker assisted breeding was one of the most effective methods to selecta resistance line. The aim of this study is to confirm the resistance gene of Dongnong 93-046 through a F8∶9 populationsfrom a cross between Dongnong 93-046 and Pin1246，to map the resistance gene，and to develop associated markers. The resultsshowed resistance line number∶ susceptible line number is 1∶1 in the F8∶9 generation，which showed that the resistance ofDonong 93-046 was controlled by a pair of alleles. A total of 76 SSＲ molecular markers were screened between parents. A totalof 13 SSＲ markers in linkage group F were used to construct a genetic map. The single marker analysis and composite intervalanalysis results show that a Dongnong 93046 resistance gene was located near the SSＲ marker Satt114. A total of 60susceptible lines and 60 resistance lines were used to evaluate accuracy of the marker，the accuracy rate of this marker was80%. It offered a good foundation for molecular marker assisted selection.

参考文献/References:

［1］ Cho E K，Goodman Ｒ M． Strain of soybean mosaic virus: Classificationbased on virulence in resistant soybean cultivars［J］. Phytopathology，1979，69: 467-470.

［2］ Buss G Ｒ，Ma G，Chen P，et al. Ｒegistration of V94-5152 soybeangermplasm resistance to soybean mosaic virus potyvirus［J］.Crop Science，1997，37: 1987-1988.
［3］ Kiihl Ｒ，Hartwig E． Heritance of reaction to soybean mosaic virusin soybean［J］. Crop Science，1979，19: 372-375.
［4］ 李文福，刘纯燕，高运来，等. 大豆种粒斑驳抗性的遗传分析及基因定位［J］. 作物学报，2008，34( 9) : 1544-1548. ( Li WF，Liu C Y，Gao Y L，et al. Genetic Analysis and mapping of resistancegene to seed coat mottle in soybean［J］. Crop Journal，2008，34( 9) : 1544-1548. )
［5］ Fu S X，Zhan Y，Zhi H J，et al. Mapping of SMV resistance geneＲsc-7 by SSＲ markers in soybean［J］. Genetica，2006，128:63-69.
［6］ Li H C，Zhi H J，Gai J Y. Inheritance and gene mapping of resistanceto soybean mosaic virus strain SC14 in soybean［J］. Journalof Integrative Plant Biology，2006，48( 12) : 1466-1472.
［7］ 郑翠明，常汝镇，邱丽娟. 大豆对SMV3 号株系的抗性遗传分析及抗病基因的ＲAPD 标记研究［J］. 中国农业科学，2001，34( 1) : 1-4. ( Zheng C M，Chang Ｒ Z，Qiu L J. ＲAPD analysis ofresistance gene to soybean mosaic virus［J］. Chinese AgriculturalScience，2001，34( 1) : 1-4. )
［8］ 卢双勇，滕卫丽，韩英鹏，等. 大豆抗花叶病毒及耐疫霉根腐病的SSＲ 分析［J］. 大豆科学，2008，27 ( 5) : 746-750. ( Lu SY，Teng W L，Han Y P，et al. SSＲ analysis of soybean mosaic virusand phytophthora root rot［J］. Soybean Science，2008，27( 5) : 746-750. )
［9］ Lander E S，Green P，Abrahamson J. MapMaker: An interactivecomputer package for constructing primary genetic linkage maps ofexperimental and natural population［J］. Genomics，1987，1:174-181.
［10］ 滕卫丽. 大豆抗花叶病遗传、细胞超微结构分析及基因定位［D］. 哈尔滨: 东北农业大学，2006. ( Teng W L. Inheritance ofresistance to SMV cellular ultastructure analysis and resistancegene mapping in soybean［D］. Harbin: Northeast Agricultural University，2006. )
［11］ Yu Y. GＲFLP and microsatellite mapping of a gene for soybeanmosaic virus resistance［J］. Phytopathology，1994，84 ( 1 ) :60-6479.
［12］ Gore M A，Hayes A J，Jeong S C，et al. Mapping tightly linkedgenes controlling potyvirus infection at the Ｒsv1 and Ｒpv1 region insoybean［J］. Genome，2002，45: 592-599.
［13］ Shi A，Chen P Y，Li X，et al. Genetic confirmation of 2 independentgenes for resistance to soybean mosaic virus in J05 soybeanusing SSＲ markers［J］. Journal of Heredity，2008，99( 6) :598-603

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备注/Memo

基金项目: 黑龙江省普通高等学校新世纪优秀人才培养计划( 1253-NCET-005) 。第一作者简介: 韩英鹏( 1978-) ，男，教授，博导，主要从事大豆遗传育种工作。E-mail: hyp234286@ aliyun. com。通讯作者: 李文滨( 1958-) ，男，教授，博导，主要从事大豆遗传育种研究和分子生物技术研究。E-mail: wenbinli@ yahoo. com。

更新日期/Last Update: 2016-06-28