[1]王鼎慧,李永光,李真,等.转GmHAP3-17基因大豆的抗旱性分析[J].大豆科学,2015,34(02):249-254.[doi:10.11861/j.issn.1000-9841.2015.02.0249]
　WANG Ding-hui,LI Yong-guang,LI Zhen,et al.Aanalysis of Drought Tolerance in Transgenic Soybean with GmHAP3-17[J].Soybean Science,2015,34(02):249-254.[doi:10.11861/j.issn.1000-9841.2015.02.0249]
点击复制

转GmHAP3-17基因大豆的抗旱性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年02期 页码: 249-254 栏目: 出版日期: 2015-04-25
Title:
Aanalysis of Drought Tolerance in Transgenic Soybean with GmHAP3-17
作者:
王鼎慧1; 2; 李永光1; 李真1; 李文滨1
1辽宁师范大学 生命科学学院，辽宁 大连 116029；?
2东北农业大学大豆生物学教育部重点实验室/东北农业大学农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030
Author(s):
WANG Ding-hui1; 2;  LI Yong-guang1;  LI Zhen1;  LI Wen-bin1
1College of Life Sciences, Liaoning Normal University, Dalian 116029, China;?
2 Key Laboratory of Soybean Biology in Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry, Northeast Agricultural University, Harbin 150030,China
关键词:
GmHAP3-17; 大豆; 抗旱性; 基因功能分析
Keywords:
GmHAP3-17;  Soybean;  Drought tolerance;  Gene function analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0249
文献标志码:
A
摘要:
GmHAP3-17基因是一个与AtHAP3-1同源的转录因子，本研究利用超表达GmHAP3-17大豆，在模拟干旱条件下以及田间干旱试验下进行了转基因大豆的抗旱性分析。结果显示：在大棚盆栽模拟干旱胁迫条件下,转基因大豆H16、H18、H26比野生型大豆WT根系发达，主根长且侧根较多，植株生长状态比对照好，叶片枯萎发生较迟、程度较轻。转基因大豆与野生型相比，MDA含量低，叶片伤害度较轻，叶片含水量较高。进一步研究表明，在田间自然干旱胁迫下，转基因大豆同样表现出较好的生长状况，较低的叶片伤害度和较高的叶片含水量。在自然干旱条件下，与野生型大豆相比，转GmHAP3-17基因大豆表现为根系发达、扎根深、侧根多，增加了株高、主茎节数、有效分枝数。H26株系的有效荚数、单株粒数、单株粒重、百粒重均比野生型提高明显，表现出了显著的产量优势。上述结果表明：GmHAP3-17基因具有正调控干旱的功能，因而该基因将成为培育抗旱转基因作物的一个有效的基因资源。
Abstract:
The present research work was mainly aim at analysing the morphological and physiological response under drought stress in transgenic soybean with GmHAP3-17. The results showed that under water-limited conditions of greenhouse, comparing with wild-type, the H16, H18 and H26 lines of transgenic soybean appeared better growth status, wilting later and milder, longer taproot, more lateral roots, lower degree of leaf damage, lower concentration of MDA and higher content of leaf relative water. Future experiment proved that under natural drought conditions of dry regions, comparing with wild-type, the root system of transgenic soybeans developed well, grew rapidly, and showed longer taproot, more lateral roots. The plants of transgenic soybean were higher in plant height, more in the number of branch and main stem node, more in pods and kernels, larger in seed size and hundred-grain weight. These consequences indicated that the GmHAP3-17 gene functions were positive regulation after drought stress treatment and thus, it could be useful in molecular breeding as effective genetic sources of transgenic crops.

参考文献/References:

［1］Bruce W B, Edmeades G O, Barker T C.Molecular and physiological approaches to maize improvement for drought tolerance［J］Journal of Experimental Botany, 2002, 53:13-25

［2］刘强，赵南明，Yamaguchi Shinozaki K, 等.DREB转录因子在提高植物抗逆性中的作用［J］科学通报，2000, 45(1): 11-16(Li Q, Zhao N M, Yamaguchi-Shinozaki K, et al. Function of DREB transcription factors in improving the plant resistance［J］, Science Bulletin, 2000, 45(1): 11-16
［3］YamaguchiShinozaki K, Kasuga M, Liu Q. Biological mechanisms of drought stress response［J］JIRCAS Working Report, 2002, 9: 1-8
［4］Donald E N, Peter P R, Tom R A. Plant nuclear factor Y (NF.Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres［J］Proceeding of the National Academy of Science of the United States of America, 2007, 104(42):16450-16455
［5］Mantovani R.The molecular biology of the CCAAT.binding factor NF.Y［J］Gene, 1999, 239:15-27
［6］Siefers N, Dang K K, Kumimoto R W.Tissue-specific expression patterns of Arabidopsis NF Y transcription factors suggest potential for extensive combinatorial complexity［J］ Plant Physiology- 2009, 149(2):625-641
［7］Ni Z, Hu Z, Jiang Q, et al. GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress［J］ Plant Molecular Biology, 2013, 82(1-2):113-29
［8］Pennisi E. Plant genetics Getting to the root of drought responses［J］Science, 2008, 11:171-173
［9］Huang C Y, Kuchel H, Edwards J. A DNA.based method for studying root responses to drought in field-grown wheat genotypes［J］Scientific Reports, 2013, 3:3194
［10］LeRudulier D, Strom A R, Dandekar A M.Molecular biology of osmoregulation［J］ Science, 1989, 224:1064-1068
［11］Ni Z, Hu Z, Jiang Q, et al.GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress［J］Plant Molecular Biology, 2013,82(1-2):113-129
［12］Wang C, Zhang D W, Wang Y C.A glycine-rich RNA-binding protein can mediate physiological responses in transgenic plants under salt stress［J］Molecular Biology Reports, 2012, 39(2):1047-1053
［13］Gadjev I, Vanderauwera S, Gechev T S, et al.Transcriptomic footprints disclose specificity of reactive oxygen species signaling in Arabidopsis［J］Plant Physiology, 2006, 141(2):436-445

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：中国博士后科学基金(2014M551205)；国家自然科学基金(31201228)。

第一作者简介：王鼎慧（1986-），女，硕士，主要从事植物遗传与育种研究。E-mail:13125926318@163.com。
通讯作者：李文滨(1958-)，男，教授，博导，主要从事大豆遗传育种研究。E-mail:wenbinli@neauedu.cn。

更新日期/Last Update: 2015-06-07