[1]葛红莲,张福丽,李俐俐.复合光合菌剂对大豆幼苗生理生化特性和根际土壤酶活性的影响[J].大豆科学,2014,33(05):713-717.[doi:10.11861/j.issn.1000-9841.2014.05.0713]
　GE Hong-lian,ZHANG Fu-li,LI Li-li.Effects of Composite Photosynthetic Bacteria PS11 on Physiological-biochemical Characteristics and Rhizosphere Soil Enzyme Activities of Soybean Seedling[J].Soybean Science,2014,33(05):713-717.[doi:10.11861/j.issn.1000-9841.2014.05.0713]
点击复制

复合光合菌剂对大豆幼苗生理生化特性和根际土壤酶活性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年05期 页码: 713-717 栏目: 出版日期: 2014-10-25
Title:
Effects of Composite Photosynthetic Bacteria PS11 on Physiological-biochemical Characteristics and Rhizosphere Soil Enzyme Activities of Soybean Seedling
文章编号:
1000-9841.2014.05.0713
作者:
葛红莲; 张福丽; 李俐俐
周口师范学院 生命科学与农学学院，河南 周口 466001
Author(s):
GE Hong-lian; ZHANG Fu-li; LI Li-li
School of Life Science and Agronomy,Zhoukou Normal University,Zhoukou 466001,China
关键词:
复合光合菌剂; 大豆; 根系活力; 土壤酶; 生理生化特性
Keywords:
Composite photosynthetic bacteria; Soybean; Root activity; Soil enzyme; Physiological and biochemical characters
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0713
文献标志码:
A
摘要:
采用106，107，108，109，101CFU.mL-15种浓度复合光合菌剂PS11灌根处理大豆幼苗，研究不同浓度复合光合菌剂PS11对大豆生理生化指标和土壤酶活性的影响。结果表明：与对照相比，106~109CFU.mL-1浓度的PS11可显著提高大豆幼苗光合色素含量,增强叶片的光合能力,增加生物量及根系活力，激活大豆幼苗抗氧化酶和土壤酶活性；随着菌液浓度的升高，PS11对大豆的促生效果及对土壤酶活性的激活作用减弱，菌液浓度1010CFU.mL-1时大豆根系活力、类胡萝卜素的含量显著低于对照；复合光合菌剂PS11浓度为107~108CFU.mL-1时对大豆幼苗和根际土壤酶活性多项指标增效作用明显。
Abstract:
Abstract: Soybean seedling were treated with 106,l07,108,109?and 1010CFU.mL-1composite photosynthetic bacteria PS11 by root irrigation to study the effect on physiological and biochemical characters of soybean seedling and its rhizosphere soil enzyme activities.The results showed that compared to the control,root irrigation treatment with 106-109CFU.mL-1PS11 significantly increased photosynthetic pigment contents of soybean leaves,enhanced photosynthetic capacity of soybean leaves,and improved plant biomass and root activity.Meanwhile,root irrigation treatment with PS11 activated the activities of antioxidant enzyme of soybean seedlings.In addition,PS11 treatment significantly improved activities of rhizosphere soil enzyme.With PS11 concentration reached to 1010CFU.mL-1,the growth-promoting effect to soybean seedling and activation to soil enzyme were weakened,and the root activity and carotin content were much lower than the control.The promotion effect of 107-108CFU.mL-1-PS11 was the most significant compared to the control.In conclusion,PS11 concentration of 107-108CFU.mL-1was the best for soybean growth and improving the soil enzyme activities by considering comprehensively the influences of different concentrations.

参考文献/References:

［1］陈怀珠，杨守臻，孙祖东，等.春大豆新品种桂春豆104的选育及高产栽培技术[J].南方农业学报，2013,44（8)：1273-1276.（Chen H Z,Yang S Z,Sun Z D,et al.Breeding of spring soybean variety Guichundou 104 and its cultivation technique[J].Journal of Southern Agriculture,2013,44(8):1273-1276.)

［2］焦扬，葛慧玲，李周密，等.基于扩散指数的中国大豆需求量增长率预警分析[J].东北林业大学学报，2013,44（8)：138-142.（Jiao Y,Ge H L,Li Z M,et al.Early-warning of soybean demand growth rate in China based on diffusion index[J].Journal of Northeast Agricultural University,2013,44(8):138-142.)
［3］Sasaki K,Watanabe W,Noparatnaraporn N.Applications of photosynthetic bacteria for medical and environmental fields[J].Seibutsu-kogaku Kaishi,2002,80:234-236.
［4］Sasaki K,Watanabe M,Suda Y,et al.Applications of photosynthetic bacteria for medical fields[J].Journal of Bioscience and Bioengineering,2005,100(5):481-488.
［5］Katsuda T，Lababpour A，Shimahara K,et al.Astaxanthin production by Haematococcus pluvialis under illumination with LEDs[J].Enzyme and Microbial Technology，2004，35：81-86.
［6］KuoF S,Chien Y H,Chen C J.Effects of light sources on growth and carotenoid content of photosynthetic bacteria Rhodopseudomonas palustris[J].Bioresource Technology，2012，113：315-318.
［7］赵志平，聂鑫，丁杰，等.光合细菌研究进展[J].四川理工学院学报(自然科学版)，2012，25（5)：1-5.（Zhao Z P,Nie X,Ding J,et al.Research progresses on photosynthetic bacteria[J].Journal of Sichuan University of Science ＆ Engineering(Natural Science Editton),2012,25(5):1-5.)
［8］王馥迪，李征，黄秋生，等.光合细菌在植物上的应用现状及展望[J].广东蚕业，2012，46（2)：36-38.（Wang F D,Li Z,Huang Q S,et al.Application status and prospect of photosynthetic bacteria on plant[J].Guangdong Canye,2012,46(2):36-38.)
［9］王秋菊，焦峰，崔战利，等.光合细菌浸种对水稻种子萌发的影响[J].作物杂志，2012（3)：126-129.（Wang Q J,Jiao F,Cui Z L,et al.Influence of soaking seeds with PSB solution on germination of rice seed[J].Crops,2012(3):126-129.)
［10］王秋菊.光合细菌对苗期水稻抗氧化作用的影响[J].黑龙江农业科学，2009（6)：33-35.（Wang Q J.Effect of photosynthetic bacteria on rice seedling antioxidant function[J].Heilongjiang Agricultural Sciences,2009(6):33-35.)
［11］武丽娜.光合细菌对水培黄瓜苗期生长的影响[J].安徽农业科学，2012，40（11)：6409-6410.（Wu L N.Influence of PSB(photosynthetic bacteria)on the growth of hydroponic cucumber seedlings[J].Anhui Agricultural Sciences,2012,40(11):6409-6410.)
［12］韩云哲，朴雪梅，许震宇，等.光合细菌对水稻生育、产量及效益影响的研究[J].吉林农业科学,2012，37（5)：6-8.(Han Y Z,Piao X M,Xu Z Y,et al.Studies on effect of photosynthetic bacteria on growth,yield and benefit of rice[J].Jilin Agricultural Sciences,2012,37（5):6-8.)
［13］张景节，张素平，李亚芹，等.施用光合细菌对大棚作物生长性状和产量的影响[J].河北农业科学，2008，12（2)：30-32.(Zhang J J,Zhang S P,Li Y Q,et al.Effects of photosynthetic bacteria on growth properties and yield of plastic house crop[J].Hebei Agricultural Sciences,2008,12(2):30-32.)
［14］白红娟，肖根林，贾万利，等.光合细菌提高污灌土壤中酶活性的研究[J].工业安全与环保，2011,37（6)：13-15.(Bai H J,Xiao G L,Jia W L,et al.Study on the increase of contaminated soil enzyme activities using photosynthetic bacteria[J].Industrial Safety and Environmental Protection,2011,37(6):13-15.)
［15］张信娣，曹慧，徐冬青，等.光合细菌和有机肥对土壤主要微生物类群和土壤酶活性的影响[J].土壤，2008,40（3)：443-447.（Zhang X D,Cao H,Xu D Q,et al.Effect of photosynthetic bacteria and organic fertilizer on soil microorganisms and soil enzyme activities[J].Soil,2008,40(3):443-447.)
［16］曹恩珲，侯宪文，李光义，等.复合菌剂对盆栽番茄土壤理化性质及微生物活性的影响[J].生态环境学报，2011,20（5)：875-880.（Cao E H,Hou X W,Li G Y,et al.Effect of combination bacteria on soil physicochemical properties and soil microbial activity by pot tomato experiment[J].Ecology and Environmental Sciences,2011,20(5):875-880.)
［17］李秀平，李穆，年海，等.甘蔗／大豆间作对甘蔗和大豆产量与品质的影响[J].东北农业大学报，2012，43（7)：42-46.（Li X P,Li M,Nian H,et al.Effect of sugarcane／soybean intercropping on growth,yield and quality of sugarcane and soybean[J].Journal of Northeast Agricultural University,2012,43(7):42-46.)
［18］刘萍，李明军.植物生理学实验技术[M].北京：科学出版社，2007.（Liu P,Li M J.Plant physiology experimental techniques[M].Beijing:Science Press，2007:35-188.)
［19］李合生.植物生理生化实验原理和技术[M].北京：高等教育出版社，2000：98-90.（Li H S.Plant physiology and biochemistry experiments:Principle and Technology[M].Beijing:Higher Education Press,2000:261.)
［20］鲍士旦.土壤农化分析［M］.北京：中国农业出版社，2000.(Bao S D.Soil agrochemical analysis[M].Beijing:China Agriculture Press,2000.)
［21］邵双，崔心栋.光合细菌促进黄瓜幼苗生长作用的研究[J].沈阳化工大学学报，2010，24（3)：215-218.(Shao S,Cui X D.Effect of photosynthetic bacteria on the growth of cucumber seedlings[J].Journal of Shenyang Institute of Chemical Technology,2010,24(3):215-218.)
［22］彭智平，李文英，徐培智，等.光合菌剂对菜心产量和品质的效应评价[J].广东农业科学，2012,39（1):11-13.(Peng Z P,Li W Y,Xiu P Z,et al.Effect of photosynthetic bacteria agent on yield and quality of flowering Chinese cabbage[J].Guangdong Agricultural Sciences,2012,39（1):11-13.)
［23］王秋菊，崔战利，张少良，等.光合细菌在水稻上的施用方法及作用机理研究[J].中国农学通报，2006，22(1)：176-178.（Wang Q J,Cui Z L,Zhang S L,et al.Study of applying methods of photosynthetic bacteria on paddy rice[J].Chinese Agricultural Science Bulletin,2006,22(1):176-178.)
［24］崔战利，王秋菊，杨会青，等.光合细菌培养液对苗期白菜UV-C辐射的防护作用初探[J].热带农业科学，2009，29(3)：10-14.(Cui Z L,Wang Q J,Yang H Q,et al.Effect of culture solution of photosynthetic bacteria on Chinese cabbage seedlings treated with UV-C radiation[J].Chinese Journal of Tropical Agriculture,2009,29(3):10-14.)
［25］戴建平，程菊娥，刘勇，等.光合细菌PSB07-15对辣椒及土壤中甲氰菊酯残留的生物修复[J].生态环境学报，2010，19(10)：2441-2444.（Dai J P,Cheng J E,Liu Y,et al.Biodegradation of fenpropahtrin by photosynthetic bacteria strain PSB07-15 in chili(Capsicum annuum linn)and soil[J].Ecology and Environmental Sciences,2010,19(10):2441-2444.)
［26］王秋菊，崔战利.光合细菌在番茄上的应用研究[J].黑龙江八一农垦大学学报，2005，17（6)：13-17.(Wang Q J,Cui Z L.Study of applying of photosynthetic bacteria on tomato[J].Journal of Heilongjiang August First Land Reclamation University,2005,17(6):13-17.)
［27］宫占元，张国庆，于文莹，等.哈茨木霉对水稻幼苗根际土壤微生物和酶活性的影响[J].干旱地区农业研究，2013，31（4)：167-171.(Gong Z Y,Zhang G Q,Yu W Y,et al.Effect of Trichoderma harzianum on microorganism and enzymatic activities in rhizosphere soil during rice seedling stage[J].Agricultural Research in the Arid Areas,2013,31(4):167-171.)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(05):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(05):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(05):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(05):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(05):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(05):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：河南省科技计划（132300410032)；河南省教育厅科学技术重点资助项目（2006210010，13B180271)。

第一作者简介：葛红莲（1976-)，女，硕士,副教授，主要从事微生物生态及微生物菌肥的开发研究。E-mail：gehonglian2003@126.com。

更新日期/Last Update: 2014-12-27