[1]莫金钢,马建,张丽辉,等.干旱胁迫对大豆种子萌发的影响[J].大豆科学,2014,33(05):701-704.[doi:10.11861/j.issn.1000-9841.2014.05.0701]
　MO Jin-gang,MA Jian,ZHANG Li-hui,et al.Effect of Drought Stress on Germination of Soybean[J].Soybean Science,2014,33(05):701-704.[doi:10.11861/j.issn.1000-9841.2014.05.0701]
点击复制

干旱胁迫对大豆种子萌发的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年05期 页码: 701-704 栏目: 出版日期: 2014-10-25
Title:
Effect of Drought Stress on Germination of Soybean
文章编号:
1000-9841.2014.05.0701
作者:
莫金钢1; 2; 马建1; 张丽辉2; 王丕武1
1.吉林农业大学 农学院，吉林 长春 130118；
2.长春师范大学 生命科学学院，吉林 长春 130032
Author(s):
MO Jin-gang1; 2; MA Jian1; ZHANG Li-hui2; WANG Pi-wu1
1.College of Agronomy,Jilin Agricultural University,Changchun 130118,China;
2.College of Life Science,Changchun Normal University,Changchun 130032,China
关键词:
大豆; 根系突变体; 干旱胁迫; 萌发; 耐旱性
Keywords:
Soybean; Root mutant; Drought stress; Germination; Drought resistance
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0701
文献标志码:
A
摘要:
为了考察干旱胁迫对大豆种子萌发的影响，以聚乙二醇（PEG-6000）作为渗透剂模拟干旱胁迫，研究了在6种不同浓度（0、5%、10%、15%、20%、25%）PEG渗透胁迫下大豆吉农18（JN18）及其根系突变体（M18）种子萌发的特征和幼苗生长的状况。结果表明：干旱胁迫对大豆种子萌发和幼苗生长均有较强的抑制作用，JN18和M18的发芽率、发芽势、发芽指数和活力指数均随着PEG处理浓度增加呈下降趋势，且在20%PEG处理下，M18的发芽率、发芽指数和活力指数显著高于JN18（P<0.05）；JN18和M18长势均与PEG浓度呈负相关，且二者在15%PEG处理下的根系性状存在极显著差异（P<0.01），同时，二者胚芽长差异显著（P<0.05）。初步认定M18较JN18具有更强的耐旱性。
Abstract:
To evaluate the effects of drought stress on seed germination and seedling growth of soybean,drought stress simulated by different concentrations of polyethylene glycol(PEG-6000),0,5%,10%,15%,20% and 25% of PEG-6000 were applied at soybean germination stage as drought stress conditions,and the germination rate,germination potential,germination index,vigor index,taproot length,number of lateral roots,total length of lateral roots and plumule length of JN18 and its root mutant M18 were analyzed,taking JN18,a wild-type line as control.The results showed that seed germination and seedling growth of soybean were significantly inhibited by drought stress in this experiment.With the strengthening of drought stress,seeds germination rate,germination index and vigor index were significantly reduced,taproot length,total length of lateral toots and plumule length of the seedlings were shortened,the number of lateral roots,root weight,lateral root weight and plumule weight were decreased.The results showed that M18 had higher seeds germination rate,germination index and vigor index under the same PEG treatment.M18 also showed a strong growth advantage under the same drought stress.Many indices of M18 were higher than JN18,including taproot length,number of lateral roots,plumule length etc..It was suggested that the ability of drought resistance of M18 was more superior than JN18.

参考文献/References:

［1］朱鹏，韩阳，阮亚男.不同大豆品种苗期抗旱性比较[J].大豆科学，2008，27（4）：711-714.(Zhu P,Han Y,Ruan Y N.Comparsion on drought resistance of diferent soybean varieties at seedling stage[J].Soybean Science,2008,27(4):711-714.)

[2]蒲伟凤，李桂兰，张敏，等.干旱胁迫对野生和栽培大豆根系特征及生理指标的影响[J].大豆科学，2010，29（4）：615-622.(Pu W F,Li G L,Zhang M,et al.Effects of drought stress on root characteristics and physiological indexes of Glycine soja and Glycine max[J].Soybean Science,2010,29(4):615-622.)
[3]孙景宽，张文辉，张洁明,等.种子萌发期4种植物对干旱胁迫的响应及其抗旱性评价研究[J].西北植物学报，2006（9）：1811-1818.(Sun J K,Zhang W H,Zhang J M，et al.Response to droughty stresses and drought-resistances evaluation of four species during seed germination[J].Acta Botanica Boreali-Occidentalia Sinica,2006(9):1811-1818.)
[4]Michael B E,Kaufmann M R.The osmotic potential of polyethy lent glycol 6000[J].Plant Physiology,1973,51:914-916.
[5]石德成，殷立娟.盐(NaCl)与碱 (Na2CO 3)对星星草胁迫作用的差异[J].植物学报，1993，35（2）：144-149.(Shi D C,Yin L J.Difference between salt(NaCl) and alkaline(Na2CO3) stresses on Puccinellia Tenuiflora Scribn.Etmerr.plants[J].Acta Botanica Sinica,1993,35(2):144-149.)
[6]Bouslama M,Schapaugh W T Jr.Stress tolerance in soybean.I.Evaluation of three screening techniques for heat and drought tolerance[J].Crop Science,1984,24(5):933-937.
[7]高小宽，刘国杰，白丽荣.聚乙二醇(PEG)模拟干旱胁迫对野生大豆与栽培大豆萌发的影响[J].大豆科学，2012，31（6）：1027-1029.(Gao X K,Liu G J,Bai L R.Effect of polyethylene glycol(PEG) simulated drought stress on seed germination of wild and cultivated soybeans[J].Soybean Science,2012,31(6):1027-1029.)
[8]Benjamin J G,Nielsen D C.Water deficit effects on root distribution of soybean,field pea and chickpea[J].Field Crops Research,2006,97(2):248-253
[9]Stolf-Moreira R,Medri M E,Neumaier N,et al.Soybean physiology and gene expression during drought[J].Genetics and Molecular Research,2010,9(4):1946-1956.
[10]武丽娜，赵泓，刘凡.蔬菜抗渗透胁迫基因工程研究进展[J].分子植物育种，2008，6（6）：1167-1174.(Wu L N,Zhao H,Liu F.Advances in genetic engineering of vegetables for osmotic stress resistance[J].Molecular Plant Breeding,2008,6(6):1167-1174.)
[11]任学良，李国柱，程文林，等.绿豆突变体的抗旱性研究[J].核农学报，2004，18（2）：100-103.(Ren X L,Li G Z,Chen W L,et al.Drought resistance characters of induced mung bean mutants[J].Acta Agrlculturae Nucleatae Sinica,2004,18(2):100-103.)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(05):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(05):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(05):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(05):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(05):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(05):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：吉林省科技厅重点科技支撑项目（20090203）。

第一作者简介：莫金钢（1979-），男，博士，实验师，主要从事生物技术在作物育种中的应用研究。E-mail:mojingang@163.com。
通讯作者：王丕武（1958-），男，博士，教授，主要从事生物技术与作物分子育种研究。E-mail:peiwuw@163.com。

更新日期/Last Update: 2014-12-26