[1]贾钰莹,蒋滢,赵强,等.农杆菌介导超高产大豆子叶节遗传转化研究[J].大豆科学,2014,33(05):634-637.[doi:10.11861/j.issn.1000-9841.2014.05.0634]
　JIA Yu-ying,JIANG Ying,ZHAO Qiang,et al.Study on Agrobacterium-mediated Transformation System of Super-high-yielding Soybean Cotyledon Node[J].Soybean Science,2014,33(05):634-637.[doi:10.11861/j.issn.1000-9841.2014.05.0634]
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农杆菌介导超高产大豆子叶节遗传转化研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年05期 页码: 634-637 栏目: 出版日期: 2014-10-25
Title:
Study on Agrobacterium-mediated Transformation System of Super-high-yielding Soybean Cotyledon Node
文章编号:
1000-9841.2014.05.0634
作者:
贾钰莹1; 蒋滢2; 赵强1; 谢甫绨1; 于翠梅1
１.沈阳农业大学 农学院，辽宁 沈阳 110866；
２.沈阳师范大学 生化与化学与生命科学学院,辽宁 沈阳 110034
Author(s):
JIA Yu-ying1; JIANG Ying2; ZHAO Qiang1; XIE Fu-ti1; YU Cui-mei1
1.College of Agronomy,Shenyang Agricultural University,Shenyang 110866,China;
2.College of Chemistry and Life Science,Shenyang Normal University,Shenyang 110034,China
关键词:
超高产大豆; 根癌农杆菌; 子叶节; 转化
Keywords:
Super-high-yielding soybeans; Agrobacterium tumefaciens; Cotyledonary node; Transformation
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0634
文献标志码:
A
摘要:
为建立一个大豆子叶节高效遗传转化体系用于大豆基因工程育种，以超高产大豆品种为材料，子叶节为外植体进行了遗传转化，研究了农杆菌侵染的菌液浓度、侵染时间、共培养基中乙酰丁香酮浓度及共培养时间等影响农杆菌转化的因素。结果表明：适宜转化的侵染和共培养条件为侵染菌液浓度OD600=0.5，侵染时间30 min，共培养基含有乙酰丁香酮浓度200 μmol.L-1，共培养时间为3～4 d。并以此条件对不同基因型超高产大豆品种进行农杆菌转化，结果显示大豆基因型对农杆菌的敏感性存在显著性差异，以沈农9号最敏感，沈农12次之，中黄35最差。
Abstract:
To establish a high frequency genetic transformation system of soybean cotyledon node for soybean genetic engineering,Agrobacterium concentration,infection time,co-cultivation time and acetosyringone concentration of co-culture medium during soybean cotyledonary node transformation were studied by using soybean cultivars with super-high-yielding.The results showed that the optimal conditions were Agrobacterium concentration OD600=0.5,infection time 30 min,co-cultivation time 3-4 days and acetosyringone concentration 200 μmol.L-1.Using the optimized transformation procedure,different genotypes of super-high-yielding soybeans were transformed and the sensitivity of soybean genotypes to Agrobacterium tumefaciens was significant differently.Shennong 9 was the most sensitive material,followed by Shennong 12,and Zhonghuang 35 was the least sensitive material.

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备注/Memo

基金项目：转基因生物新品种培育重大专项（2013ZX08004-004-003-004）。

第一作者简介：贾钰莹(1987-)，女，博士，主要从事大豆遗传转化研究。E-mail：jiayuyinggood@163.com。
通讯作者：于翠梅(1974-)，女，博士，副教授，主要从事大豆遗传转化与基因工程。E-mail：yucuimei@163.com。

更新日期/Last Update: 2014-12-24